Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye

Detalhes bibliográficos
Autor(a) principal: Ambrósio Jr.,Renato
Data de Publicação: 2011
Outros Autores: Ahmad,Habeeb, Caldas,Diogo, Canedo,Ana Laura Caiado, Valbon,Bruno, Guerra,Frederico Procopio, Lima,Acácio Alves de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Oftalmologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802011000600014
Resumo: Different applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.
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spelling Novel use of trypan blue in ocular surface staining: redefining implications for this vital dyeEye diseases/surgeryOphthalmologic surgical proceduresTrypan blue/diagnostic useStaining and labeling/methodsCase reportsDifferent applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.Sociedade Brasileira de Oftalmologia2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802011000600014Revista Brasileira de Oftalmologia v.70 n.6 2011reponame:Revista Brasileira de Oftalmologia (Online)instname:Sociedade Brasileira de Oftalmologia (SBO)instacron:SBO10.1590/S0034-72802011000600014info:eu-repo/semantics/openAccessAmbrósio Jr.,RenatoAhmad,HabeebCaldas,DiogoCanedo,Ana Laura CaiadoValbon,BrunoGuerra,Frederico ProcopioLima,Acácio Alves de Souzaeng2012-02-01T00:00:00Zoai:scielo:S0034-72802011000600014Revistahttps://rbo.emnuvens.com.br/rbo/indexhttps://old.scielo.br/oai/scielo-oai.phpsob@sboportal.org.br||rbo@sboportal.org.br1982-85510034-7280opendoar:2012-02-01T00:00Revista Brasileira de Oftalmologia (Online) - Sociedade Brasileira de Oftalmologia (SBO)false
dc.title.none.fl_str_mv Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
spellingShingle Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
Ambrósio Jr.,Renato
Eye diseases/surgery
Ophthalmologic surgical procedures
Trypan blue/diagnostic use
Staining and labeling/methods
Case reports
title_short Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_full Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_fullStr Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_full_unstemmed Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_sort Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
author Ambrósio Jr.,Renato
author_facet Ambrósio Jr.,Renato
Ahmad,Habeeb
Caldas,Diogo
Canedo,Ana Laura Caiado
Valbon,Bruno
Guerra,Frederico Procopio
Lima,Acácio Alves de Souza
author_role author
author2 Ahmad,Habeeb
Caldas,Diogo
Canedo,Ana Laura Caiado
Valbon,Bruno
Guerra,Frederico Procopio
Lima,Acácio Alves de Souza
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ambrósio Jr.,Renato
Ahmad,Habeeb
Caldas,Diogo
Canedo,Ana Laura Caiado
Valbon,Bruno
Guerra,Frederico Procopio
Lima,Acácio Alves de Souza
dc.subject.por.fl_str_mv Eye diseases/surgery
Ophthalmologic surgical procedures
Trypan blue/diagnostic use
Staining and labeling/methods
Case reports
topic Eye diseases/surgery
Ophthalmologic surgical procedures
Trypan blue/diagnostic use
Staining and labeling/methods
Case reports
description Different applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802011000600014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802011000600014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0034-72802011000600014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Oftalmologia
publisher.none.fl_str_mv Sociedade Brasileira de Oftalmologia
dc.source.none.fl_str_mv Revista Brasileira de Oftalmologia v.70 n.6 2011
reponame:Revista Brasileira de Oftalmologia (Online)
instname:Sociedade Brasileira de Oftalmologia (SBO)
instacron:SBO
instname_str Sociedade Brasileira de Oftalmologia (SBO)
instacron_str SBO
institution SBO
reponame_str Revista Brasileira de Oftalmologia (Online)
collection Revista Brasileira de Oftalmologia (Online)
repository.name.fl_str_mv Revista Brasileira de Oftalmologia (Online) - Sociedade Brasileira de Oftalmologia (SBO)
repository.mail.fl_str_mv sob@sboportal.org.br||rbo@sboportal.org.br
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