Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye

Detalhes bibliográficos
Autor(a) principal: Ambrósio Junior, Renato [UNIFESP]
Data de Publicação: 2011
Outros Autores: Ahmad, Habeeb, Caldas, Diogo, Canedo, Ana Laura Caiado, Valbon, Bruno, Guerra, Frederico Procopio, Lima Filho, Acácio Alves de Souza [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0034-72802011000600014
http://repositorio.unifesp.br/handle/11600/6698
Resumo: Different applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.
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spelling Novel use of trypan blue in ocular surface staining: redefining implications for this vital dyeNova aplicação do azul de tripano para superfície ocular: redefinindo corantes vitaisEye diseasesOphthalmologic surgical proceduresTrypan blueStaining and labelingCase reportsOftalmopatiasProcedimentos cirúrgicos oftalmológicosAzul tripanoColoração e rotulagemRelatos de casosDifferent applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.Diferentes aplicações do azul de tripano (AT) foram descritas para cirurgia intraocular, com elevados patamares de eficácia e segurança. Neste relato, é descrito a aplicação do AT como corante vital para superfíce ocular, de modo a estudar a integridade das células da superfície corneana e conjuntival na superfície ocular. Tal abordagem permite um diagnóstico mais sensível de desordens da superfície ocular, destacando-se disfunção lacrimal ou síndrome do olho seco. O AT tem propriedades distintas da fluoresceína, com a qual se mantém estável em solução permitindo a técnica de coloração dupla que é introduzida.Instituto de Olhos Renato AmbrósioCorneal Tomography and Biomechanics Study GroupDoheny Eye InstitutePrice Vision GroupUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPM, São PauloSciELOSociedade Brasileira de OftalmologiaInstituto de Olhos Renato AmbrósioCorneal Tomography and Biomechanics Study GroupDoheny Eye InstitutePrice Vision GroupUniversidade Federal de São Paulo (UNIFESP)Ambrósio Junior, Renato [UNIFESP]Ahmad, HabeebCaldas, DiogoCanedo, Ana Laura CaiadoValbon, BrunoGuerra, Frederico ProcopioLima Filho, Acácio Alves de Souza [UNIFESP]2015-06-14T13:43:21Z2015-06-14T13:43:21Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion408-410application/pdfhttp://dx.doi.org/10.1590/S0034-72802011000600014Revista Brasileira de Oftalmologia. Sociedade Brasileira de Oftalmologia, v. 70, n. 6, p. 408-410, 2011.10.1590/S0034-72802011000600014S0034-72802011000600014.pdf0034-7280S0034-72802011000600014http://repositorio.unifesp.br/handle/11600/6698engRevista Brasileira de Oftalmologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T13:28:36Zoai:repositorio.unifesp.br/:11600/6698Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T13:28:36Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
Nova aplicação do azul de tripano para superfície ocular: redefinindo corantes vitais
title Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
spellingShingle Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
Ambrósio Junior, Renato [UNIFESP]
Eye diseases
Ophthalmologic surgical procedures
Trypan blue
Staining and labeling
Case reports
Oftalmopatias
Procedimentos cirúrgicos oftalmológicos
Azul tripano
Coloração e rotulagem
Relatos de casos
title_short Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_full Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_fullStr Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_full_unstemmed Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
title_sort Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye
author Ambrósio Junior, Renato [UNIFESP]
author_facet Ambrósio Junior, Renato [UNIFESP]
Ahmad, Habeeb
Caldas, Diogo
Canedo, Ana Laura Caiado
Valbon, Bruno
Guerra, Frederico Procopio
Lima Filho, Acácio Alves de Souza [UNIFESP]
author_role author
author2 Ahmad, Habeeb
Caldas, Diogo
Canedo, Ana Laura Caiado
Valbon, Bruno
Guerra, Frederico Procopio
Lima Filho, Acácio Alves de Souza [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Olhos Renato Ambrósio
Corneal Tomography and Biomechanics Study Group
Doheny Eye Institute
Price Vision Group
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Ambrósio Junior, Renato [UNIFESP]
Ahmad, Habeeb
Caldas, Diogo
Canedo, Ana Laura Caiado
Valbon, Bruno
Guerra, Frederico Procopio
Lima Filho, Acácio Alves de Souza [UNIFESP]
dc.subject.por.fl_str_mv Eye diseases
Ophthalmologic surgical procedures
Trypan blue
Staining and labeling
Case reports
Oftalmopatias
Procedimentos cirúrgicos oftalmológicos
Azul tripano
Coloração e rotulagem
Relatos de casos
topic Eye diseases
Ophthalmologic surgical procedures
Trypan blue
Staining and labeling
Case reports
Oftalmopatias
Procedimentos cirúrgicos oftalmológicos
Azul tripano
Coloração e rotulagem
Relatos de casos
description Different applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2015-06-14T13:43:21Z
2015-06-14T13:43:21Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0034-72802011000600014
Revista Brasileira de Oftalmologia. Sociedade Brasileira de Oftalmologia, v. 70, n. 6, p. 408-410, 2011.
10.1590/S0034-72802011000600014
S0034-72802011000600014.pdf
0034-7280
S0034-72802011000600014
http://repositorio.unifesp.br/handle/11600/6698
url http://dx.doi.org/10.1590/S0034-72802011000600014
http://repositorio.unifesp.br/handle/11600/6698
identifier_str_mv Revista Brasileira de Oftalmologia. Sociedade Brasileira de Oftalmologia, v. 70, n. 6, p. 408-410, 2011.
10.1590/S0034-72802011000600014
S0034-72802011000600014.pdf
0034-7280
S0034-72802011000600014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Oftalmologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 408-410
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Oftalmologia
publisher.none.fl_str_mv Sociedade Brasileira de Oftalmologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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