Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells

Detalhes bibliográficos
Autor(a) principal: Kaleka,Camila Cohen
Data de Publicação: 2018
Outros Autores: Zucconi,Eder, Vieira,Tierri da Silva, Secco,Mariane, Ferretti,Mário, Cohen,Moisés
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Ortopedia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000500557
Resumo: ABSTRACT Objective: The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs). Methods: AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific). Results: The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group. Conclusion: These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.
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spelling Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cellsCartilage diseasesKneeArthroscopyArticular cartilageMesenchymal stem cellsMesenchymal stem cell transplantationABSTRACT Objective: The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs). Methods: AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific). Results: The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group. Conclusion: These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.Sociedade Brasileira de Ortopedia e Traumatologia2018-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000500557Revista Brasileira de Ortopedia v.53 n.5 2018reponame:Revista Brasileira de Ortopedia (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1016/j.rboe.2018.07.009info:eu-repo/semantics/openAccessKaleka,Camila CohenZucconi,EderVieira,Tierri da SilvaSecco,MarianeFerretti,MárioCohen,Moiséseng2018-10-16T00:00:00Zoai:scielo:S0102-36162018000500557Revistahttp://www.rbo.org.br/https://old.scielo.br/oai/scielo-oai.php||rbo@sbot.org.br1982-43780102-3616opendoar:2018-10-16T00:00Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
title Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
spellingShingle Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
Kaleka,Camila Cohen
Cartilage diseases
Knee
Arthroscopy
Articular cartilage
Mesenchymal stem cells
Mesenchymal stem cell transplantation
title_short Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
title_full Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
title_fullStr Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
title_full_unstemmed Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
title_sort Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
author Kaleka,Camila Cohen
author_facet Kaleka,Camila Cohen
Zucconi,Eder
Vieira,Tierri da Silva
Secco,Mariane
Ferretti,Mário
Cohen,Moisés
author_role author
author2 Zucconi,Eder
Vieira,Tierri da Silva
Secco,Mariane
Ferretti,Mário
Cohen,Moisés
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kaleka,Camila Cohen
Zucconi,Eder
Vieira,Tierri da Silva
Secco,Mariane
Ferretti,Mário
Cohen,Moisés
dc.subject.por.fl_str_mv Cartilage diseases
Knee
Arthroscopy
Articular cartilage
Mesenchymal stem cells
Mesenchymal stem cell transplantation
topic Cartilage diseases
Knee
Arthroscopy
Articular cartilage
Mesenchymal stem cells
Mesenchymal stem cell transplantation
description ABSTRACT Objective: The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs). Methods: AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific). Results: The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group. Conclusion: These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000500557
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000500557
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.rboe.2018.07.009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
dc.source.none.fl_str_mv Revista Brasileira de Ortopedia v.53 n.5 2018
reponame:Revista Brasileira de Ortopedia (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Revista Brasileira de Ortopedia (Online)
collection Revista Brasileira de Ortopedia (Online)
repository.name.fl_str_mv Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv ||rbo@sbot.org.br
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