Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine

Detalhes bibliográficos
Autor(a) principal: Sumarta, Ni Putu Mira
Data de Publicação: 2021
Outros Autores: Kamadjaja, David Buntoro, Hendrijantini, Nike, Danudiningrat, Coen Pramono, Rantam, Fedik Abdul
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
Texto Completo: https://revista.uepb.edu.br/PBOCI/article/view/631
Resumo: Objective: To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA). Results: The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3. Conclusion: Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.
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spelling Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative MedicineMesenchymal Stem CellsCord Blood Stem Cell TransplantationCartilageObjective: To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA). Results: The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3. Conclusion: Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2021-11-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/631Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 21 (2021); e0034Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 21 (2021); e00341983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/631/336Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSumarta, Ni Putu Mira Kamadjaja, David Buntoro Hendrijantini, Nike Danudiningrat, Coen Pramono Rantam, Fedik Abdul 2021-11-11T19:39:41Zoai:ojs.pkp.sfu.ca:article/631Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2021-11-11T19:39:41Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false
dc.title.none.fl_str_mv Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
title Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
spellingShingle Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
Sumarta, Ni Putu Mira
Mesenchymal Stem Cells
Cord Blood Stem Cell Transplantation
Cartilage
title_short Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
title_full Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
title_fullStr Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
title_full_unstemmed Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
title_sort Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
author Sumarta, Ni Putu Mira
author_facet Sumarta, Ni Putu Mira
Kamadjaja, David Buntoro
Hendrijantini, Nike
Danudiningrat, Coen Pramono
Rantam, Fedik Abdul
author_role author
author2 Kamadjaja, David Buntoro
Hendrijantini, Nike
Danudiningrat, Coen Pramono
Rantam, Fedik Abdul
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Sumarta, Ni Putu Mira
Kamadjaja, David Buntoro
Hendrijantini, Nike
Danudiningrat, Coen Pramono
Rantam, Fedik Abdul
dc.subject.por.fl_str_mv Mesenchymal Stem Cells
Cord Blood Stem Cell Transplantation
Cartilage
topic Mesenchymal Stem Cells
Cord Blood Stem Cell Transplantation
Cartilage
description Objective: To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA). Results: The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3. Conclusion: Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/631
url https://revista.uepb.edu.br/PBOCI/article/view/631
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/631/336
dc.rights.driver.fl_str_mv Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
dc.source.none.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 21 (2021); e0034
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 21 (2021); e0034
1983-4632
1519-0501
reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
instname:Universidade Estadual da Paraíba (UEPB)
instacron:UEPB
instname_str Universidade Estadual da Paraíba (UEPB)
instacron_str UEPB
institution UEPB
reponame_str Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
collection Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
repository.name.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)
repository.mail.fl_str_mv pboci.editoria@gmail.com || alessandrouepb@gmail.com
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