Influence of mononuclear cell therapy on disc degeneration in rabbits

Detalhes bibliográficos
Autor(a) principal: Fávaro,Rodrigo Caldonazzo
Data de Publicação: 2016
Outros Autores: Arruda,André de Oliveira, Vialle,Luiz Roberto Gomes, Vialle,Emiliano Neves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Ortopedia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000600707
Resumo: ABSTRACT OBJECTIVE: The objective of this research was to evaluate the influence of autologous mononuclear stem cells injections on histological changes of collagen in the fibrous annulus of the intervertebral disk after experimental injury. METHODS: 32 New Zealand rabbits were submitted to intervertebral disk puncture, followed by intradiscal injection of mononuclear cells from the iliac crest versus saline injection in the following time periods: two months after the injury (SC2M and SS2M), two weeks (SC2W and SS2W) immediately after injury (SCCP and SSCP), and without inducing degeneration (SCSP and SSSP). Two months after cell therapy, the animals were euthanized and collagen changes in the intervertebral discs were histologically evaluated. RESULTS: There were significant differences in ELAF between SS2W and SS2S groups (p = 0.018). This difference was due to an increase in type I collagen in SS2W group (56.7%) compared to SC2S (13.28%). CONCLUSION: Treatment with mononuclear mesenchymal stem cells reduced changes in the type I and III collagen distribution in rabbits AF degenerated discs up to two weeks after the induction of degeneration.
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spelling Influence of mononuclear cell therapy on disc degeneration in rabbitsCollagenIntervertebral diskCell and tissue-based therapyHistologyRabbitABSTRACT OBJECTIVE: The objective of this research was to evaluate the influence of autologous mononuclear stem cells injections on histological changes of collagen in the fibrous annulus of the intervertebral disk after experimental injury. METHODS: 32 New Zealand rabbits were submitted to intervertebral disk puncture, followed by intradiscal injection of mononuclear cells from the iliac crest versus saline injection in the following time periods: two months after the injury (SC2M and SS2M), two weeks (SC2W and SS2W) immediately after injury (SCCP and SSCP), and without inducing degeneration (SCSP and SSSP). Two months after cell therapy, the animals were euthanized and collagen changes in the intervertebral discs were histologically evaluated. RESULTS: There were significant differences in ELAF between SS2W and SS2S groups (p = 0.018). This difference was due to an increase in type I collagen in SS2W group (56.7%) compared to SC2S (13.28%). CONCLUSION: Treatment with mononuclear mesenchymal stem cells reduced changes in the type I and III collagen distribution in rabbits AF degenerated discs up to two weeks after the induction of degeneration.Sociedade Brasileira de Ortopedia e Traumatologia2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000600707Revista Brasileira de Ortopedia v.51 n.6 2016reponame:Revista Brasileira de Ortopedia (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1016/j.rboe.2016.10.003info:eu-repo/semantics/openAccessFávaro,Rodrigo CaldonazzoArruda,André de OliveiraVialle,Luiz Roberto GomesVialle,Emiliano Neveseng2016-12-19T00:00:00Zoai:scielo:S0102-36162016000600707Revistahttp://www.rbo.org.br/https://old.scielo.br/oai/scielo-oai.php||rbo@sbot.org.br1982-43780102-3616opendoar:2016-12-19T00:00Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv Influence of mononuclear cell therapy on disc degeneration in rabbits
title Influence of mononuclear cell therapy on disc degeneration in rabbits
spellingShingle Influence of mononuclear cell therapy on disc degeneration in rabbits
Fávaro,Rodrigo Caldonazzo
Collagen
Intervertebral disk
Cell and tissue-based therapy
Histology
Rabbit
title_short Influence of mononuclear cell therapy on disc degeneration in rabbits
title_full Influence of mononuclear cell therapy on disc degeneration in rabbits
title_fullStr Influence of mononuclear cell therapy on disc degeneration in rabbits
title_full_unstemmed Influence of mononuclear cell therapy on disc degeneration in rabbits
title_sort Influence of mononuclear cell therapy on disc degeneration in rabbits
author Fávaro,Rodrigo Caldonazzo
author_facet Fávaro,Rodrigo Caldonazzo
Arruda,André de Oliveira
Vialle,Luiz Roberto Gomes
Vialle,Emiliano Neves
author_role author
author2 Arruda,André de Oliveira
Vialle,Luiz Roberto Gomes
Vialle,Emiliano Neves
author2_role author
author
author
dc.contributor.author.fl_str_mv Fávaro,Rodrigo Caldonazzo
Arruda,André de Oliveira
Vialle,Luiz Roberto Gomes
Vialle,Emiliano Neves
dc.subject.por.fl_str_mv Collagen
Intervertebral disk
Cell and tissue-based therapy
Histology
Rabbit
topic Collagen
Intervertebral disk
Cell and tissue-based therapy
Histology
Rabbit
description ABSTRACT OBJECTIVE: The objective of this research was to evaluate the influence of autologous mononuclear stem cells injections on histological changes of collagen in the fibrous annulus of the intervertebral disk after experimental injury. METHODS: 32 New Zealand rabbits were submitted to intervertebral disk puncture, followed by intradiscal injection of mononuclear cells from the iliac crest versus saline injection in the following time periods: two months after the injury (SC2M and SS2M), two weeks (SC2W and SS2W) immediately after injury (SCCP and SSCP), and without inducing degeneration (SCSP and SSSP). Two months after cell therapy, the animals were euthanized and collagen changes in the intervertebral discs were histologically evaluated. RESULTS: There were significant differences in ELAF between SS2W and SS2S groups (p = 0.018). This difference was due to an increase in type I collagen in SS2W group (56.7%) compared to SC2S (13.28%). CONCLUSION: Treatment with mononuclear mesenchymal stem cells reduced changes in the type I and III collagen distribution in rabbits AF degenerated discs up to two weeks after the induction of degeneration.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000600707
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000600707
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.rboe.2016.10.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
dc.source.none.fl_str_mv Revista Brasileira de Ortopedia v.51 n.6 2016
reponame:Revista Brasileira de Ortopedia (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Revista Brasileira de Ortopedia (Online)
collection Revista Brasileira de Ortopedia (Online)
repository.name.fl_str_mv Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv ||rbo@sbot.org.br
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