Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates

Detalhes bibliográficos
Autor(a) principal: Lima,Janaína S.
Data de Publicação: 2018
Outros Autores: Braga,Kaoane Regina G. S., Vieira,Camila A., Souza,Wérika Weryanne R., Chávez-Pavoni,Juliana Helena, Araújo,Claudinéia de, Goulart,Letícia S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000100028
Resumo: ABSTRACT Introduction: Candida albicans is the most common etiologic agent of fungal vaginitis. These yeasts produce secreted aspartyl proteinases encoded by a family of 10 genes (SAP1-10). Objective: The purpose of this study was to analyze the presence of genes SAP1-7 in vulvovaginal C. albicans. Materials and method: The study included 26 C. albicans vaginal isolates. Detection of aspartyl proteases genes (SAP1-7) was performed by polymerase chain reaction (PCR). Results: The most frequent gene in C. albicans isolated from colonization was SAP6 (93.33%), and from infection, SAP7 (100%). We observed a statistical difference (p = 0.049) in SAP1 gene frequency between isolates from vulvovaginal colonization and infection. Conclusion: High frequency of SAP genes was observed in vulvovaginal C. albicans. The results suggest SAP1 participation in vulvovaginal candidiasis infection.
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spelling Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolatesvulvovaginal candidiasisCandida albicansvirulence factorsABSTRACT Introduction: Candida albicans is the most common etiologic agent of fungal vaginitis. These yeasts produce secreted aspartyl proteinases encoded by a family of 10 genes (SAP1-10). Objective: The purpose of this study was to analyze the presence of genes SAP1-7 in vulvovaginal C. albicans. Materials and method: The study included 26 C. albicans vaginal isolates. Detection of aspartyl proteases genes (SAP1-7) was performed by polymerase chain reaction (PCR). Results: The most frequent gene in C. albicans isolated from colonization was SAP6 (93.33%), and from infection, SAP7 (100%). We observed a statistical difference (p = 0.049) in SAP1 gene frequency between isolates from vulvovaginal colonization and infection. Conclusion: High frequency of SAP genes was observed in vulvovaginal C. albicans. The results suggest SAP1 participation in vulvovaginal candidiasis infection.Sociedade Brasileira de Patologia Clínica2018-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000100028Jornal Brasileiro de Patologia e Medicina Laboratorial v.54 n.1 2018reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20180006info:eu-repo/semantics/openAccessLima,Janaína S.Braga,Kaoane Regina G. S.Vieira,Camila A.Souza,Wérika Weryanne R.Chávez-Pavoni,Juliana HelenaAraújo,Claudinéia deGoulart,Letícia S.eng2018-04-11T00:00:00Zoai:scielo:S1676-24442018000100028Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2018-04-11T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false
dc.title.none.fl_str_mv Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
title Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
spellingShingle Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
Lima,Janaína S.
vulvovaginal candidiasis
Candida albicans
virulence factors
title_short Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
title_full Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
title_fullStr Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
title_full_unstemmed Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
title_sort Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates
author Lima,Janaína S.
author_facet Lima,Janaína S.
Braga,Kaoane Regina G. S.
Vieira,Camila A.
Souza,Wérika Weryanne R.
Chávez-Pavoni,Juliana Helena
Araújo,Claudinéia de
Goulart,Letícia S.
author_role author
author2 Braga,Kaoane Regina G. S.
Vieira,Camila A.
Souza,Wérika Weryanne R.
Chávez-Pavoni,Juliana Helena
Araújo,Claudinéia de
Goulart,Letícia S.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima,Janaína S.
Braga,Kaoane Regina G. S.
Vieira,Camila A.
Souza,Wérika Weryanne R.
Chávez-Pavoni,Juliana Helena
Araújo,Claudinéia de
Goulart,Letícia S.
dc.subject.por.fl_str_mv vulvovaginal candidiasis
Candida albicans
virulence factors
topic vulvovaginal candidiasis
Candida albicans
virulence factors
description ABSTRACT Introduction: Candida albicans is the most common etiologic agent of fungal vaginitis. These yeasts produce secreted aspartyl proteinases encoded by a family of 10 genes (SAP1-10). Objective: The purpose of this study was to analyze the presence of genes SAP1-7 in vulvovaginal C. albicans. Materials and method: The study included 26 C. albicans vaginal isolates. Detection of aspartyl proteases genes (SAP1-7) was performed by polymerase chain reaction (PCR). Results: The most frequent gene in C. albicans isolated from colonization was SAP6 (93.33%), and from infection, SAP7 (100%). We observed a statistical difference (p = 0.049) in SAP1 gene frequency between isolates from vulvovaginal colonization and infection. Conclusion: High frequency of SAP genes was observed in vulvovaginal C. albicans. The results suggest SAP1 participation in vulvovaginal candidiasis infection.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000100028
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000100028
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/1676-2444.20180006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
dc.source.none.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial v.54 n.1 2018
reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
instname:Sociedade Brasileira de Patologia (SBP)
instacron:SBP
instname_str Sociedade Brasileira de Patologia (SBP)
instacron_str SBP
institution SBP
reponame_str Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
collection Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
repository.name.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)
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