Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories

Detalhes bibliográficos
Autor(a) principal: Silva,Glaucielen G.
Data de Publicação: 2017
Outros Autores: Gomes,Clayson M., Dellavance,Alessandra, Francescantonio,Paulo Luiz C., Andrade,Luís Eduardo C., Cruvinel,Wilson M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000600368
Resumo: ABSTRACT Introduction: The detection of autoantibodies in HEp-2 cells represents a relevant tool for the diagnosis of autoimmune diseases, especially rheumatic autoimmune diseases. As a result of the methodological advances, the technique gradually increased the sensitivity, as well as the need for standardization. Objective: To evaluate the implementation of the Brazilian Consensus recommendations for autoantibody determination in HEp-2 cells. Methods: A structured form in a virtual platform was filled in by experts in clinical laboratories that carry out the methodology across the country. The questionnaire addressed the adoption of the Brazilian Consensus guidelines, detailing the technical aspects, quality control, the strategy for reading slides and the release of reports. Results: The study included 53 laboratories responsible for more than 300,000 antinuclear antibody (ANA) tests/month; more than half (58.5%) reported fully adopting the recommendations of the Brazilian Consensus. The majority (83.1%) used the 1:80 for screening dilution, and 75.5% of laboratories, perform education and quality control programs. Only 39.6% reported using more than one kit brand to perform the test, and 32.1% did not report observing all phases of the cell cycle during slide reading. The study also detected some heterogeneity among participants in the identification of patterns. Conclusion: The results confirm the adoption of the Brazilian Consensus recommendations by most of participating laboratories, although with variable extent. There is need for improvement in some aspects, especially those related to the quality control.
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spelling Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratoriesindirect fluorescent-antibody techniqueautoantibodiesABSTRACT Introduction: The detection of autoantibodies in HEp-2 cells represents a relevant tool for the diagnosis of autoimmune diseases, especially rheumatic autoimmune diseases. As a result of the methodological advances, the technique gradually increased the sensitivity, as well as the need for standardization. Objective: To evaluate the implementation of the Brazilian Consensus recommendations for autoantibody determination in HEp-2 cells. Methods: A structured form in a virtual platform was filled in by experts in clinical laboratories that carry out the methodology across the country. The questionnaire addressed the adoption of the Brazilian Consensus guidelines, detailing the technical aspects, quality control, the strategy for reading slides and the release of reports. Results: The study included 53 laboratories responsible for more than 300,000 antinuclear antibody (ANA) tests/month; more than half (58.5%) reported fully adopting the recommendations of the Brazilian Consensus. The majority (83.1%) used the 1:80 for screening dilution, and 75.5% of laboratories, perform education and quality control programs. Only 39.6% reported using more than one kit brand to perform the test, and 32.1% did not report observing all phases of the cell cycle during slide reading. The study also detected some heterogeneity among participants in the identification of patterns. Conclusion: The results confirm the adoption of the Brazilian Consensus recommendations by most of participating laboratories, although with variable extent. There is need for improvement in some aspects, especially those related to the quality control.Sociedade Brasileira de Patologia Clínica2017-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000600368Jornal Brasileiro de Patologia e Medicina Laboratorial v.53 n.6 2017reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20170059info:eu-repo/semantics/openAccessSilva,Glaucielen G.Gomes,Clayson M.Dellavance,AlessandraFrancescantonio,Paulo Luiz C.Andrade,Luís Eduardo C.Cruvinel,Wilson M.eng2018-02-05T00:00:00Zoai:scielo:S1676-24442017000600368Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2018-02-05T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false
dc.title.none.fl_str_mv Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
title Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
spellingShingle Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
Silva,Glaucielen G.
indirect fluorescent-antibody technique
autoantibodies
title_short Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
title_full Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
title_fullStr Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
title_full_unstemmed Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
title_sort Implementation of the ANA HEp-2 consensus guidelines in Brazilian clinical laboratories
author Silva,Glaucielen G.
author_facet Silva,Glaucielen G.
Gomes,Clayson M.
Dellavance,Alessandra
Francescantonio,Paulo Luiz C.
Andrade,Luís Eduardo C.
Cruvinel,Wilson M.
author_role author
author2 Gomes,Clayson M.
Dellavance,Alessandra
Francescantonio,Paulo Luiz C.
Andrade,Luís Eduardo C.
Cruvinel,Wilson M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Glaucielen G.
Gomes,Clayson M.
Dellavance,Alessandra
Francescantonio,Paulo Luiz C.
Andrade,Luís Eduardo C.
Cruvinel,Wilson M.
dc.subject.por.fl_str_mv indirect fluorescent-antibody technique
autoantibodies
topic indirect fluorescent-antibody technique
autoantibodies
description ABSTRACT Introduction: The detection of autoantibodies in HEp-2 cells represents a relevant tool for the diagnosis of autoimmune diseases, especially rheumatic autoimmune diseases. As a result of the methodological advances, the technique gradually increased the sensitivity, as well as the need for standardization. Objective: To evaluate the implementation of the Brazilian Consensus recommendations for autoantibody determination in HEp-2 cells. Methods: A structured form in a virtual platform was filled in by experts in clinical laboratories that carry out the methodology across the country. The questionnaire addressed the adoption of the Brazilian Consensus guidelines, detailing the technical aspects, quality control, the strategy for reading slides and the release of reports. Results: The study included 53 laboratories responsible for more than 300,000 antinuclear antibody (ANA) tests/month; more than half (58.5%) reported fully adopting the recommendations of the Brazilian Consensus. The majority (83.1%) used the 1:80 for screening dilution, and 75.5% of laboratories, perform education and quality control programs. Only 39.6% reported using more than one kit brand to perform the test, and 32.1% did not report observing all phases of the cell cycle during slide reading. The study also detected some heterogeneity among participants in the identification of patterns. Conclusion: The results confirm the adoption of the Brazilian Consensus recommendations by most of participating laboratories, although with variable extent. There is need for improvement in some aspects, especially those related to the quality control.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-01
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000600368
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dc.publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
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Sociedade Brasileira de Patologia Clínica
dc.source.none.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial v.53 n.6 2017
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