Molecular characterization of Enterobacteriaceae resistant to carbapenem antimicrobials

• Autor(a) principal: Marques,Jaciane B.
• Data de Publicação: 2015
• Outros Autores: Bonez,Pauline C., Agertt,Vanessa A., Flores,Vanessa C., Dalmolin,Tanise V., Rossi,Grazielle G., Dal Forno,Nara Lucia F., Bianchini,Bianca V., Mizdal,Caren R., Siqueira,Fallon S., Righi,Roselene A., Meneghetti,Bettina H., Trindade,Priscila A., Santos,Roberto C. V., Campos,Marli M. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442015000300162
• topic: Enterobacteriaceae; carbapenemase; phenotypic methods; molecular methods

ABSTRACT The present study aimed to genotypically and phenotypically characterize clinical isolates of carbapenem-resistant Enterobacteriaceae collected from inpatients at the University Hospital of Santa Maria, during seven months. Among the clinical isolates subjected to the modified Hodge test (MHT), 62.5% were positive, indicating possible production of carbapenemase. Polymerase chain reaction (PCR) demonstrated that blaKPC was the most frequently found gene (31%), followed by blaIMP (12.5%). Combined use of the methods is needed to identify carbapenem resistance in enterobacteria to prevent their spread and control the infections caused by these organisms.