Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442013000300002 |
Resumo: | INTRODUCTION: Cryptococcus neoformans and Cryptococcus gattii are encapsulated basidiomycetous yeasts with worldwide distribution. They cause cryptococcosis with features of systemic infection, affecting the central nervous system, lungs and skin in humans and animals. These fungi present numerous virulence factors that allow them to invade the host and multiply, among which extracellular enzyme capacity and microbial adaptation to different temperatures are worth mentioning. OBJECTIVE: To evaluate the production of protease and investigate possible differences in thermotolerance and urease activity in clinical and environmental yeast isolates. MATERIAL AND METHODS: Culture methods and Pz analysis were applied to assess urease and protease, whereas the optical density method was used to analyze biological activity in thermotolerance. RESULTS: There was no significant results as to microbial growth at the tested temperatures (25º, 37º and 42ºC). It was observed that clinical specimens grew better than environmental ones at elevated temperatures. As to C. neoformans, the moderate production of urease enzyme prevailed in both clinical and environmental isolates within 24h or 48h. Moreover, there was significant production on the seventh day of reading. The best reading time for viewing protease production in both isolates and species was the seventh day: 96% clinical samples and 94% environmental isolates. CONCLUSION: Further studies are required in order to investigate the virulence factors of C. neoformans and C. gattii cerebrospinal isolates from patients with meningoencephalitis and environmental samples from Sergipe. Furthermore, a higher technical accuracy and statistical precision are indispensable. |
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Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolatesCryptococcus neoformansfungal proteinsmycologyyeastCryptococcus gattiiINTRODUCTION: Cryptococcus neoformans and Cryptococcus gattii are encapsulated basidiomycetous yeasts with worldwide distribution. They cause cryptococcosis with features of systemic infection, affecting the central nervous system, lungs and skin in humans and animals. These fungi present numerous virulence factors that allow them to invade the host and multiply, among which extracellular enzyme capacity and microbial adaptation to different temperatures are worth mentioning. OBJECTIVE: To evaluate the production of protease and investigate possible differences in thermotolerance and urease activity in clinical and environmental yeast isolates. MATERIAL AND METHODS: Culture methods and Pz analysis were applied to assess urease and protease, whereas the optical density method was used to analyze biological activity in thermotolerance. RESULTS: There was no significant results as to microbial growth at the tested temperatures (25º, 37º and 42ºC). It was observed that clinical specimens grew better than environmental ones at elevated temperatures. As to C. neoformans, the moderate production of urease enzyme prevailed in both clinical and environmental isolates within 24h or 48h. Moreover, there was significant production on the seventh day of reading. The best reading time for viewing protease production in both isolates and species was the seventh day: 96% clinical samples and 94% environmental isolates. CONCLUSION: Further studies are required in order to investigate the virulence factors of C. neoformans and C. gattii cerebrospinal isolates from patients with meningoencephalitis and environmental samples from Sergipe. Furthermore, a higher technical accuracy and statistical precision are indispensable.Sociedade Brasileira de Patologia Clínica2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442013000300002Jornal Brasileiro de Patologia e Medicina Laboratorial v.49 n.3 2013reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442013000300002info:eu-repo/semantics/openAccessBarbosa Junior,Antonio MarcioSantos,Bruno Fernandes de OliveiraCarvalho,Erick de OliveiraMélo,Dângelly Lins Figuerôa Martins deTrindade,Rita de CássiaStoianoff,Maria Aparecida de Resendeeng2013-08-29T00:00:00Zoai:scielo:S1676-24442013000300002Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2013-08-29T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
title |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
spellingShingle |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates Barbosa Junior,Antonio Marcio Cryptococcus neoformans fungal proteins mycology yeast Cryptococcus gattii |
title_short |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
title_full |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
title_fullStr |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
title_full_unstemmed |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
title_sort |
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates |
author |
Barbosa Junior,Antonio Marcio |
author_facet |
Barbosa Junior,Antonio Marcio Santos,Bruno Fernandes de Oliveira Carvalho,Erick de Oliveira Mélo,Dângelly Lins Figuerôa Martins de Trindade,Rita de Cássia Stoianoff,Maria Aparecida de Resende |
author_role |
author |
author2 |
Santos,Bruno Fernandes de Oliveira Carvalho,Erick de Oliveira Mélo,Dângelly Lins Figuerôa Martins de Trindade,Rita de Cássia Stoianoff,Maria Aparecida de Resende |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barbosa Junior,Antonio Marcio Santos,Bruno Fernandes de Oliveira Carvalho,Erick de Oliveira Mélo,Dângelly Lins Figuerôa Martins de Trindade,Rita de Cássia Stoianoff,Maria Aparecida de Resende |
dc.subject.por.fl_str_mv |
Cryptococcus neoformans fungal proteins mycology yeast Cryptococcus gattii |
topic |
Cryptococcus neoformans fungal proteins mycology yeast Cryptococcus gattii |
description |
INTRODUCTION: Cryptococcus neoformans and Cryptococcus gattii are encapsulated basidiomycetous yeasts with worldwide distribution. They cause cryptococcosis with features of systemic infection, affecting the central nervous system, lungs and skin in humans and animals. These fungi present numerous virulence factors that allow them to invade the host and multiply, among which extracellular enzyme capacity and microbial adaptation to different temperatures are worth mentioning. OBJECTIVE: To evaluate the production of protease and investigate possible differences in thermotolerance and urease activity in clinical and environmental yeast isolates. MATERIAL AND METHODS: Culture methods and Pz analysis were applied to assess urease and protease, whereas the optical density method was used to analyze biological activity in thermotolerance. RESULTS: There was no significant results as to microbial growth at the tested temperatures (25º, 37º and 42ºC). It was observed that clinical specimens grew better than environmental ones at elevated temperatures. As to C. neoformans, the moderate production of urease enzyme prevailed in both clinical and environmental isolates within 24h or 48h. Moreover, there was significant production on the seventh day of reading. The best reading time for viewing protease production in both isolates and species was the seventh day: 96% clinical samples and 94% environmental isolates. CONCLUSION: Further studies are required in order to investigate the virulence factors of C. neoformans and C. gattii cerebrospinal isolates from patients with meningoencephalitis and environmental samples from Sergipe. Furthermore, a higher technical accuracy and statistical precision are indispensable. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442013000300002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442013000300002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-24442013000300002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.49 n.3 2013 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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