N-acetylcysteine and fructose-1,6-bisphosphate: immunomodulatory effects on mononuclear cell culture

• Autor(a) principal: Mello,Ricardo Obalski de
• Data de Publicação: 2012
• Outros Autores: Lunardelli,Adroaldo, Caberlon,Eduardo, Moraes,Cristina Machado Bragança de, Santos,Roberto Christ Vianna, Costa,Vinícius Lorini da, da Silva,Gabriela Viegas, Scherer,Patrícia da Silva, Buaes,Luiz Eduardo Coimbra, Donadio,Márcio Vinícius Fagundes, Nunes,Fernanda Bordignon, Oliveira,Jarbas Rodrigues de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442012000200007
• topic: Fructose-1; 6-bisphosphate; N-acetylcysteine; T-lymphocytes; Interleukin-1β; MCP-1

INTRODUCTION: Sepsis is a complex syndrome caused by an uncontrolled systemic inflammatory response. Inflammatory cytokines play a pivotal role in septic shock pathogenesis. Therapeutic strategies have been tested in order to modulate the excessive generation or function of sepsis mediators. OBJECTIVE: The objective of the present study was to investigate the therapeutic effect of N-acetylcysteine (NAC) and its association with fructose-1,6-bisphosphate (FBP) on T-lymphocytes proliferation, interleukin-1β (IL-1β) and monocyte chemotactic protein-1 (MCP-1) levels. MATERIAL AND METHODS: Peripheral blood mononuclear cell samples were isolated from healthy individuals. T-lymphocytes were stimulated with phytohemagglutinin for 96 hours and submitted to different concentrations of NAC or NAC associated with FBP. RESULTS: NAC (10 and 15 mM) and NAC (15 mM) associated with FBP reduced T-lymphocytes proliferation. IL-1β levels rose in the presence of both NAC (15 mM) and NAC with FBP (1.25 mM). MCP-1 levels were reduced only by NAC (15 mM) associated with FBP (1.25 mM). CONCLUSION: The results suggest that both NAC itself and NAC associated with FBP inhibit cellular proliferation, acting as potent immunomodulatory agents, which corroborates its use in the treatment of inflammatory diseases.