DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/1751 |
Resumo: | Callus culture has shown a great potential for large-scale multiplication of superior genotypes in a short period of time. The objective of this work was to establish a methodology to induce callus formation in leaf fragments of Coffea canephora Conilon cultivar, to determine the growth curve and to analyze the development of the calli. The growth curve allows to determine the suitable time to subculture the calli in a new medium. The explants were inoculated in 50% MS medium with 10 mg.L-1 thiamine, 1 mg.L-1 pyridoxine, 1 mg.L-1 nicotinic acid, 1 mg.L-1 glycine, inositol 100 mg.L-1, hydrolysed casein 100 mg.L-1 and 400 mg.L-1 malt extract, 20 g.L-1 sucrose, 8 g.L-1 agar, supplemented with IBA (10 µM), 2,4-D (20 µM) and 2iP (10 µM). To determine the growth curve, the calli were weighted up to the 60th day of culture. The callus growth curve presented sigmoidal shape, with five distinct phases. The subculture must be done on the 53th day of culture, aiming at the plant regeneration. |
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DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEECoffea canephora. Callogenesis. Growth regulatorsCallus culture has shown a great potential for large-scale multiplication of superior genotypes in a short period of time. The objective of this work was to establish a methodology to induce callus formation in leaf fragments of Coffea canephora Conilon cultivar, to determine the growth curve and to analyze the development of the calli. The growth curve allows to determine the suitable time to subculture the calli in a new medium. The explants were inoculated in 50% MS medium with 10 mg.L-1 thiamine, 1 mg.L-1 pyridoxine, 1 mg.L-1 nicotinic acid, 1 mg.L-1 glycine, inositol 100 mg.L-1, hydrolysed casein 100 mg.L-1 and 400 mg.L-1 malt extract, 20 g.L-1 sucrose, 8 g.L-1 agar, supplemented with IBA (10 µM), 2,4-D (20 µM) and 2iP (10 µM). To determine the growth curve, the calli were weighted up to the 60th day of culture. The callus growth curve presented sigmoidal shape, with five distinct phases. The subculture must be done on the 53th day of culture, aiming at the plant regeneration.Universidade Federal Rural do Semi-Árido2010-05-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1751REVISTA CAATINGA; Vol. 23 No. 1 (2010); 133-136Revista Caatinga; v. 23 n. 1 (2010); 133-1361983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAporhttps://periodicos.ufersa.edu.br/caatinga/article/view/1751/4536Alves dos Santos, Maurício ReginaldoFerreira, Maria das Graças RodriguesSarubo, Vâniainfo:eu-repo/semantics/openAccess2018-12-07T16:34:54Zoai:ojs.periodicos.ufersa.edu.br:article/1751Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:45:40.251402Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
title |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
spellingShingle |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE Alves dos Santos, Maurício Reginaldo Coffea canephora. Callogenesis. Growth regulators |
title_short |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
title_full |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
title_fullStr |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
title_full_unstemmed |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
title_sort |
DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE |
author |
Alves dos Santos, Maurício Reginaldo |
author_facet |
Alves dos Santos, Maurício Reginaldo Ferreira, Maria das Graças Rodrigues Sarubo, Vânia |
author_role |
author |
author2 |
Ferreira, Maria das Graças Rodrigues Sarubo, Vânia |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Alves dos Santos, Maurício Reginaldo Ferreira, Maria das Graças Rodrigues Sarubo, Vânia |
dc.subject.por.fl_str_mv |
Coffea canephora. Callogenesis. Growth regulators |
topic |
Coffea canephora. Callogenesis. Growth regulators |
description |
Callus culture has shown a great potential for large-scale multiplication of superior genotypes in a short period of time. The objective of this work was to establish a methodology to induce callus formation in leaf fragments of Coffea canephora Conilon cultivar, to determine the growth curve and to analyze the development of the calli. The growth curve allows to determine the suitable time to subculture the calli in a new medium. The explants were inoculated in 50% MS medium with 10 mg.L-1 thiamine, 1 mg.L-1 pyridoxine, 1 mg.L-1 nicotinic acid, 1 mg.L-1 glycine, inositol 100 mg.L-1, hydrolysed casein 100 mg.L-1 and 400 mg.L-1 malt extract, 20 g.L-1 sucrose, 8 g.L-1 agar, supplemented with IBA (10 µM), 2,4-D (20 µM) and 2iP (10 µM). To determine the growth curve, the calli were weighted up to the 60th day of culture. The callus growth curve presented sigmoidal shape, with five distinct phases. The subculture must be done on the 53th day of culture, aiming at the plant regeneration. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/1751 |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/1751 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/1751/4536 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 23 No. 1 (2010); 133-136 Revista Caatinga; v. 23 n. 1 (2010); 133-136 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674022117310464 |