DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE

Detalhes bibliográficos
Autor(a) principal: Alves dos Santos, Maurício Reginaldo
Data de Publicação: 2010
Outros Autores: Ferreira, Maria das Graças Rodrigues, Sarubo, Vânia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Caatinga
Texto Completo: https://periodicos.ufersa.edu.br/caatinga/article/view/1751
Resumo: Callus culture has shown a great potential for large-scale multiplication of superior genotypes in a short period of time. The objective of this work was to establish a methodology to induce callus formation in leaf fragments of Coffea canephora Conilon cultivar, to determine the growth curve and to analyze the development of the calli. The growth curve allows to determine the suitable time to subculture the calli in a new medium. The explants were inoculated in 50% MS medium with 10 mg.L-1 thiamine, 1 mg.L-1 pyridoxine, 1 mg.L-1 nicotinic acid, 1 mg.L-1 glycine, inositol 100 mg.L-1, hydrolysed casein 100 mg.L-1 and 400 mg.L-1 malt extract, 20 g.L-1 sucrose, 8 g.L-1 agar, supplemented with IBA (10 µM), 2,4-D (20 µM) and 2iP (10 µM). To determine the growth curve, the calli were weighted up to the 60th day of culture. The callus growth curve presented sigmoidal shape, with five distinct phases. The subculture must be done on the 53th day of culture, aiming at the plant regeneration.
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spelling DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEECoffea canephora. Callogenesis. Growth regulatorsCallus culture has shown a great potential for large-scale multiplication of superior genotypes in a short period of time. The objective of this work was to establish a methodology to induce callus formation in leaf fragments of Coffea canephora Conilon cultivar, to determine the growth curve and to analyze the development of the calli. The growth curve allows to determine the suitable time to subculture the calli in a new medium. The explants were inoculated in 50% MS medium with 10 mg.L-1 thiamine, 1 mg.L-1 pyridoxine, 1 mg.L-1 nicotinic acid, 1 mg.L-1 glycine, inositol 100 mg.L-1, hydrolysed casein 100 mg.L-1 and 400 mg.L-1 malt extract, 20 g.L-1 sucrose, 8 g.L-1 agar, supplemented with IBA (10 µM), 2,4-D (20 µM) and 2iP (10 µM). To determine the growth curve, the calli were weighted up to the 60th day of culture. The callus growth curve presented sigmoidal shape, with five distinct phases. The subculture must be done on the 53th day of culture, aiming at the plant regeneration.Universidade Federal Rural do Semi-Árido2010-05-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1751REVISTA CAATINGA; Vol. 23 No. 1 (2010); 133-136Revista Caatinga; v. 23 n. 1 (2010); 133-1361983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAporhttps://periodicos.ufersa.edu.br/caatinga/article/view/1751/4536Alves dos Santos, Maurício ReginaldoFerreira, Maria das Graças RodriguesSarubo, Vâniainfo:eu-repo/semantics/openAccess2018-12-07T16:34:54Zoai:ojs.periodicos.ufersa.edu.br:article/1751Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:45:40.251402Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true
dc.title.none.fl_str_mv DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
title DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
spellingShingle DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
Alves dos Santos, Maurício Reginaldo
Coffea canephora. Callogenesis. Growth regulators
title_short DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
title_full DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
title_fullStr DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
title_full_unstemmed DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
title_sort DETERMINATION OF CALLUS GROWTH CURVE IN CONILON COFFEE
author Alves dos Santos, Maurício Reginaldo
author_facet Alves dos Santos, Maurício Reginaldo
Ferreira, Maria das Graças Rodrigues
Sarubo, Vânia
author_role author
author2 Ferreira, Maria das Graças Rodrigues
Sarubo, Vânia
author2_role author
author
dc.contributor.author.fl_str_mv Alves dos Santos, Maurício Reginaldo
Ferreira, Maria das Graças Rodrigues
Sarubo, Vânia
dc.subject.por.fl_str_mv Coffea canephora. Callogenesis. Growth regulators
topic Coffea canephora. Callogenesis. Growth regulators
description Callus culture has shown a great potential for large-scale multiplication of superior genotypes in a short period of time. The objective of this work was to establish a methodology to induce callus formation in leaf fragments of Coffea canephora Conilon cultivar, to determine the growth curve and to analyze the development of the calli. The growth curve allows to determine the suitable time to subculture the calli in a new medium. The explants were inoculated in 50% MS medium with 10 mg.L-1 thiamine, 1 mg.L-1 pyridoxine, 1 mg.L-1 nicotinic acid, 1 mg.L-1 glycine, inositol 100 mg.L-1, hydrolysed casein 100 mg.L-1 and 400 mg.L-1 malt extract, 20 g.L-1 sucrose, 8 g.L-1 agar, supplemented with IBA (10 µM), 2,4-D (20 µM) and 2iP (10 µM). To determine the growth curve, the calli were weighted up to the 60th day of culture. The callus growth curve presented sigmoidal shape, with five distinct phases. The subculture must be done on the 53th day of culture, aiming at the plant regeneration.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/1751
url https://periodicos.ufersa.edu.br/caatinga/article/view/1751
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/1751/4536
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
dc.source.none.fl_str_mv REVISTA CAATINGA; Vol. 23 No. 1 (2010); 133-136
Revista Caatinga; v. 23 n. 1 (2010); 133-136
1983-2125
0100-316X
reponame:Revista Caatinga
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Revista Caatinga
collection Revista Caatinga
repository.name.fl_str_mv Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv patricio@ufersa.edu.br|| caatinga@ufersa.edu.br
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