Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes

Detalhes bibliográficos
Autor(a) principal: GONÇALVES,Fernanda Castanheira
Data de Publicação: 2020
Outros Autores: OLIVEIRA,Guilherme José Pimentel Lopes de, SCARDUELI,Cassio Rocha, SPIN-NETO,Rubens, STAVROPOULOS,Andreas, MARCANTONIO,Rosemary Adriana Chiérici
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242020000100205
Resumo: Abstract The aim of this study was to assess the influence of cyclosporine administration on the repair of critical-sized calvaria defects (CSDs) in rat calvaria filled with diverse biomaterials. Sixty animals were divided into two groups: the control (CTR) group (saline solution) and the cyclosporine (CCP) group (cyclosporine, 10 mg/kg/day). These medications were administered daily by gavage, beginning 15 days before the surgical procedure and lasting until the day the animals were euthanized. A CSD (5 mm Ø) was made in the calvaria of each animal, which was allocated to one of 3 subgroups, according to the biomaterial used to fill the defect: coagulum (COA), deproteinized bovine bone (DBB), or biphasic calcium phosphate ceramics of hydroxyapatite and β-phosphate tricalcium (HA/TCP). Euthanasia of the animals was performed 15 and 60 days after the surgical procedure (n = 5 animals/period/subgroup). Bone repair (formation) assessment was performed through microtomography and histometry, while the analyses of the expression of the BMP2, Osteocalcin, and TGFβ1 proteins were performed using immunohistochemistry. The CSDs not filled with biomaterials demonstrated lower bone formation in the CCP group. At 15 days, less bone formation was observed in the CSDs filled with DBB, a smaller volume of mineralized tissue was observed in the CSDs filled with HA/TCP, and the expression levels of BMP2 and osteocalcin were lower in the CCP group compared to the CTR group. The use of cyclosporine impaired bone repair in CSD, and this effect can be partially explained by the suppression of BMP2 and osteocalcin expression.
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spelling Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutesBone RegenerationBone SubstitutesImmunosuppressionAbstract The aim of this study was to assess the influence of cyclosporine administration on the repair of critical-sized calvaria defects (CSDs) in rat calvaria filled with diverse biomaterials. Sixty animals were divided into two groups: the control (CTR) group (saline solution) and the cyclosporine (CCP) group (cyclosporine, 10 mg/kg/day). These medications were administered daily by gavage, beginning 15 days before the surgical procedure and lasting until the day the animals were euthanized. A CSD (5 mm Ø) was made in the calvaria of each animal, which was allocated to one of 3 subgroups, according to the biomaterial used to fill the defect: coagulum (COA), deproteinized bovine bone (DBB), or biphasic calcium phosphate ceramics of hydroxyapatite and β-phosphate tricalcium (HA/TCP). Euthanasia of the animals was performed 15 and 60 days after the surgical procedure (n = 5 animals/period/subgroup). Bone repair (formation) assessment was performed through microtomography and histometry, while the analyses of the expression of the BMP2, Osteocalcin, and TGFβ1 proteins were performed using immunohistochemistry. The CSDs not filled with biomaterials demonstrated lower bone formation in the CCP group. At 15 days, less bone formation was observed in the CSDs filled with DBB, a smaller volume of mineralized tissue was observed in the CSDs filled with HA/TCP, and the expression levels of BMP2 and osteocalcin were lower in the CCP group compared to the CTR group. The use of cyclosporine impaired bone repair in CSD, and this effect can be partially explained by the suppression of BMP2 and osteocalcin expression.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242020000100205Brazilian Oral Research v.34 2020reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2020.vol34.0007info:eu-repo/semantics/openAccessGONÇALVES,Fernanda CastanheiraOLIVEIRA,Guilherme José Pimentel Lopes deSCARDUELI,Cassio RochaSPIN-NETO,RubensSTAVROPOULOS,AndreasMARCANTONIO,Rosemary Adriana Chiéricieng2020-02-03T00:00:00Zoai:scielo:S1806-83242020000100205Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2020-02-03T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
title Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
spellingShingle Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
GONÇALVES,Fernanda Castanheira
Bone Regeneration
Bone Substitutes
Immunosuppression
title_short Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
title_full Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
title_fullStr Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
title_full_unstemmed Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
title_sort Cyclosporine A impairs bone repair in critical defects filled with different osteoconductive bone substitutes
author GONÇALVES,Fernanda Castanheira
author_facet GONÇALVES,Fernanda Castanheira
OLIVEIRA,Guilherme José Pimentel Lopes de
SCARDUELI,Cassio Rocha
SPIN-NETO,Rubens
STAVROPOULOS,Andreas
MARCANTONIO,Rosemary Adriana Chiérici
author_role author
author2 OLIVEIRA,Guilherme José Pimentel Lopes de
SCARDUELI,Cassio Rocha
SPIN-NETO,Rubens
STAVROPOULOS,Andreas
MARCANTONIO,Rosemary Adriana Chiérici
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv GONÇALVES,Fernanda Castanheira
OLIVEIRA,Guilherme José Pimentel Lopes de
SCARDUELI,Cassio Rocha
SPIN-NETO,Rubens
STAVROPOULOS,Andreas
MARCANTONIO,Rosemary Adriana Chiérici
dc.subject.por.fl_str_mv Bone Regeneration
Bone Substitutes
Immunosuppression
topic Bone Regeneration
Bone Substitutes
Immunosuppression
description Abstract The aim of this study was to assess the influence of cyclosporine administration on the repair of critical-sized calvaria defects (CSDs) in rat calvaria filled with diverse biomaterials. Sixty animals were divided into two groups: the control (CTR) group (saline solution) and the cyclosporine (CCP) group (cyclosporine, 10 mg/kg/day). These medications were administered daily by gavage, beginning 15 days before the surgical procedure and lasting until the day the animals were euthanized. A CSD (5 mm Ø) was made in the calvaria of each animal, which was allocated to one of 3 subgroups, according to the biomaterial used to fill the defect: coagulum (COA), deproteinized bovine bone (DBB), or biphasic calcium phosphate ceramics of hydroxyapatite and β-phosphate tricalcium (HA/TCP). Euthanasia of the animals was performed 15 and 60 days after the surgical procedure (n = 5 animals/period/subgroup). Bone repair (formation) assessment was performed through microtomography and histometry, while the analyses of the expression of the BMP2, Osteocalcin, and TGFβ1 proteins were performed using immunohistochemistry. The CSDs not filled with biomaterials demonstrated lower bone formation in the CCP group. At 15 days, less bone formation was observed in the CSDs filled with DBB, a smaller volume of mineralized tissue was observed in the CSDs filled with HA/TCP, and the expression levels of BMP2 and osteocalcin were lower in the CCP group compared to the CTR group. The use of cyclosporine impaired bone repair in CSD, and this effect can be partially explained by the suppression of BMP2 and osteocalcin expression.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242020000100205
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2020.vol34.0007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.34 2020
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
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reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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