Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100225 |
Resumo: | Abstract: Despite the crucial role of osteoclasts in the physiological process of bone repair, most bone tissue engineering strategies have focused on osteoblast-biomaterial interactions. Although Biosilicate® with two crystalline phases (BioS-2P) exhibits osteogenic properties and significant bone formation, its effects on osteoclasts are unknown. This study aimed to investigate the in vitro and in vivo effects of BioS-2P on osteoclast differentiation and activity. RAW 264.7 cells were cultured in osteoclastogenic medium (OCM) or OCM conditioned with BioS-2P (OCM-BioS-2P), and the cell morphology, viability, and osteoclast differentiation were evaluated. BioS-2P scaffolds were implanted into rat calvarial defects, and the bone tissue was evaluated using tartrate-resistant acid phosphatase (TRAP) staining and RT-polymerase chain reaction (PCR) after 2 and 4 weeks to determine the gene expressions of osteoclast markers and compare them with those of the bone grown in empty defects (Control). OCM-BioS-2P favored osteoclast viability and activity, as evidenced by an increase in the TRAP-positive cells and matrix resorption. The bone tissue grown on BioS-2P scaffolds exhibited higher expression of the osteoclast marker genes (Ctsk, Mmp 9, Rank) after 2 and 4 weeks and the RankL/Opg ratio after 2 weeks. Trap gene expression was lower at 2 weeks, and a higher number of TRAP-stained areas were observed in the newly formed bone on BioS-2P scaffolds at both 2 and 4 weeks compared to the Controls. These results enhanced our understanding of the role of bioactive glass-ceramics in bone repair, and highlighted their role in the modulation of osteoclastic activities and promotion of interactions between bone tissues and biomaterials. |
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Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclastsBiocompatible MaterialsOsteoclastsBone and BonesTissue EngineeringAbstract: Despite the crucial role of osteoclasts in the physiological process of bone repair, most bone tissue engineering strategies have focused on osteoblast-biomaterial interactions. Although Biosilicate® with two crystalline phases (BioS-2P) exhibits osteogenic properties and significant bone formation, its effects on osteoclasts are unknown. This study aimed to investigate the in vitro and in vivo effects of BioS-2P on osteoclast differentiation and activity. RAW 264.7 cells were cultured in osteoclastogenic medium (OCM) or OCM conditioned with BioS-2P (OCM-BioS-2P), and the cell morphology, viability, and osteoclast differentiation were evaluated. BioS-2P scaffolds were implanted into rat calvarial defects, and the bone tissue was evaluated using tartrate-resistant acid phosphatase (TRAP) staining and RT-polymerase chain reaction (PCR) after 2 and 4 weeks to determine the gene expressions of osteoclast markers and compare them with those of the bone grown in empty defects (Control). OCM-BioS-2P favored osteoclast viability and activity, as evidenced by an increase in the TRAP-positive cells and matrix resorption. The bone tissue grown on BioS-2P scaffolds exhibited higher expression of the osteoclast marker genes (Ctsk, Mmp 9, Rank) after 2 and 4 weeks and the RankL/Opg ratio after 2 weeks. Trap gene expression was lower at 2 weeks, and a higher number of TRAP-stained areas were observed in the newly formed bone on BioS-2P scaffolds at both 2 and 4 weeks compared to the Controls. These results enhanced our understanding of the role of bioactive glass-ceramics in bone repair, and highlighted their role in the modulation of osteoclastic activities and promotion of interactions between bone tissues and biomaterials.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100225Brazilian Oral Research v.36 2022reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2022.vol36.0022info:eu-repo/semantics/openAccessBighetti-Trevisan,Rayana LongoSouza,Alann Thaffarell PortilhoTosin,Ingrid WezelBueno,Natália PierettiCrovace,Murilo CamuriBeloti,Marcio MateusRosa,Adalberto LuizFerraz,Emanuela Pradoeng2022-03-10T00:00:00Zoai:scielo:S1806-83242022000100225Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2022-03-10T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
title |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
spellingShingle |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts Bighetti-Trevisan,Rayana Longo Biocompatible Materials Osteoclasts Bone and Bones Tissue Engineering |
title_short |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
title_full |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
title_fullStr |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
title_full_unstemmed |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
title_sort |
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts |
author |
Bighetti-Trevisan,Rayana Longo |
author_facet |
Bighetti-Trevisan,Rayana Longo Souza,Alann Thaffarell Portilho Tosin,Ingrid Wezel Bueno,Natália Pieretti Crovace,Murilo Camuri Beloti,Marcio Mateus Rosa,Adalberto Luiz Ferraz,Emanuela Prado |
author_role |
author |
author2 |
Souza,Alann Thaffarell Portilho Tosin,Ingrid Wezel Bueno,Natália Pieretti Crovace,Murilo Camuri Beloti,Marcio Mateus Rosa,Adalberto Luiz Ferraz,Emanuela Prado |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Bighetti-Trevisan,Rayana Longo Souza,Alann Thaffarell Portilho Tosin,Ingrid Wezel Bueno,Natália Pieretti Crovace,Murilo Camuri Beloti,Marcio Mateus Rosa,Adalberto Luiz Ferraz,Emanuela Prado |
dc.subject.por.fl_str_mv |
Biocompatible Materials Osteoclasts Bone and Bones Tissue Engineering |
topic |
Biocompatible Materials Osteoclasts Bone and Bones Tissue Engineering |
description |
Abstract: Despite the crucial role of osteoclasts in the physiological process of bone repair, most bone tissue engineering strategies have focused on osteoblast-biomaterial interactions. Although Biosilicate® with two crystalline phases (BioS-2P) exhibits osteogenic properties and significant bone formation, its effects on osteoclasts are unknown. This study aimed to investigate the in vitro and in vivo effects of BioS-2P on osteoclast differentiation and activity. RAW 264.7 cells were cultured in osteoclastogenic medium (OCM) or OCM conditioned with BioS-2P (OCM-BioS-2P), and the cell morphology, viability, and osteoclast differentiation were evaluated. BioS-2P scaffolds were implanted into rat calvarial defects, and the bone tissue was evaluated using tartrate-resistant acid phosphatase (TRAP) staining and RT-polymerase chain reaction (PCR) after 2 and 4 weeks to determine the gene expressions of osteoclast markers and compare them with those of the bone grown in empty defects (Control). OCM-BioS-2P favored osteoclast viability and activity, as evidenced by an increase in the TRAP-positive cells and matrix resorption. The bone tissue grown on BioS-2P scaffolds exhibited higher expression of the osteoclast marker genes (Ctsk, Mmp 9, Rank) after 2 and 4 weeks and the RankL/Opg ratio after 2 weeks. Trap gene expression was lower at 2 weeks, and a higher number of TRAP-stained areas were observed in the newly formed bone on BioS-2P scaffolds at both 2 and 4 weeks compared to the Controls. These results enhanced our understanding of the role of bioactive glass-ceramics in bone repair, and highlighted their role in the modulation of osteoclastic activities and promotion of interactions between bone tissues and biomaterials. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100225 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100225 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2022.vol36.0022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.36 2022 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318328141840384 |