Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy

Detalhes bibliográficos
Autor(a) principal: TOLEDO,Gustavo Lopes
Data de Publicação: 2021
Outros Autores: SANGALETTE,Beatriz Sobrinho, PASSEROTTI,Larissa Camargo, NASCIMENTO,Juliana de Almeida, SHINOHARA,André Luis, OLIVEIRA,Alexandre Leite Rodrigues de, BUZALAF,Marília Afonso Rabelo, RODRIGUES,Antônio de Castro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307
Resumo: Abstract The loss of continuity of the nerve structure interrupts the transmission of nerve impulses and leads to the disorganization of functional activities. Many methods, as the use of neurogenic factors, aid in the process of neural regeneration by accelerating or improving peripheral nerves neoformation. The adipose tissue is abundant in the human body, and it has presented promising results in the regeneration of peripheral nerves. We carried out a randomized controlled study in 9 months, using 45 male Wistar rats, 80 days old, and the sciatic nerve was chosen for analysis. The control animals were divided into three groups – Initial group (IG), Final group (FG), and denervated group (DG) – with seven animals each. The experimental groups, with twelve animals each, were polyethylene tube filled with fat (EGF) and polyethylene tube without filling (EGwf). All groups, except IG, were submitted to 10 sessions of hyperbaric oxygen treatment of 1h 45 min in alternating days. Functional evaluation by walking-track was assessed using the Catwalk XT® software and tissues were harvested and stained with 1% toluidine blue for histological analysis. Quantitative data were first analyzed with the Kolmogorov Smirnov normality test. Comparison between the four groups was analyzed by ANOVA followed by Tukey Test. We concluded that hyperbaric oxygen therapy had positive results on morphometric and functional parameters. However, no significant differences were found regarding the use of autologous fat graft.
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spelling Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapyNerve RegenerationNeuronsNervous SystemNeuronal PlasticityPeripheral NervesAbstract The loss of continuity of the nerve structure interrupts the transmission of nerve impulses and leads to the disorganization of functional activities. Many methods, as the use of neurogenic factors, aid in the process of neural regeneration by accelerating or improving peripheral nerves neoformation. The adipose tissue is abundant in the human body, and it has presented promising results in the regeneration of peripheral nerves. We carried out a randomized controlled study in 9 months, using 45 male Wistar rats, 80 days old, and the sciatic nerve was chosen for analysis. The control animals were divided into three groups – Initial group (IG), Final group (FG), and denervated group (DG) – with seven animals each. The experimental groups, with twelve animals each, were polyethylene tube filled with fat (EGF) and polyethylene tube without filling (EGwf). All groups, except IG, were submitted to 10 sessions of hyperbaric oxygen treatment of 1h 45 min in alternating days. Functional evaluation by walking-track was assessed using the Catwalk XT® software and tissues were harvested and stained with 1% toluidine blue for histological analysis. Quantitative data were first analyzed with the Kolmogorov Smirnov normality test. Comparison between the four groups was analyzed by ANOVA followed by Tukey Test. We concluded that hyperbaric oxygen therapy had positive results on morphometric and functional parameters. However, no significant differences were found regarding the use of autologous fat graft.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307Brazilian Oral Research v.35 2021reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2021.vol35.0138info:eu-repo/semantics/openAccessTOLEDO,Gustavo LopesSANGALETTE,Beatriz SobrinhoPASSEROTTI,Larissa CamargoNASCIMENTO,Juliana de AlmeidaSHINOHARA,André LuisOLIVEIRA,Alexandre Leite Rodrigues deBUZALAF,Marília Afonso RabeloRODRIGUES,Antônio de Castroeng2021-12-15T00:00:00Zoai:scielo:S1806-83242021000100307Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2021-12-15T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
title Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
spellingShingle Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
TOLEDO,Gustavo Lopes
Nerve Regeneration
Neurons
Nervous System
Neuronal Plasticity
Peripheral Nerves
title_short Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
title_full Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
title_fullStr Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
title_full_unstemmed Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
title_sort Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
author TOLEDO,Gustavo Lopes
author_facet TOLEDO,Gustavo Lopes
SANGALETTE,Beatriz Sobrinho
PASSEROTTI,Larissa Camargo
NASCIMENTO,Juliana de Almeida
SHINOHARA,André Luis
OLIVEIRA,Alexandre Leite Rodrigues de
BUZALAF,Marília Afonso Rabelo
RODRIGUES,Antônio de Castro
author_role author
author2 SANGALETTE,Beatriz Sobrinho
PASSEROTTI,Larissa Camargo
NASCIMENTO,Juliana de Almeida
SHINOHARA,André Luis
OLIVEIRA,Alexandre Leite Rodrigues de
BUZALAF,Marília Afonso Rabelo
RODRIGUES,Antônio de Castro
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv TOLEDO,Gustavo Lopes
SANGALETTE,Beatriz Sobrinho
PASSEROTTI,Larissa Camargo
NASCIMENTO,Juliana de Almeida
SHINOHARA,André Luis
OLIVEIRA,Alexandre Leite Rodrigues de
BUZALAF,Marília Afonso Rabelo
RODRIGUES,Antônio de Castro
dc.subject.por.fl_str_mv Nerve Regeneration
Neurons
Nervous System
Neuronal Plasticity
Peripheral Nerves
topic Nerve Regeneration
Neurons
Nervous System
Neuronal Plasticity
Peripheral Nerves
description Abstract The loss of continuity of the nerve structure interrupts the transmission of nerve impulses and leads to the disorganization of functional activities. Many methods, as the use of neurogenic factors, aid in the process of neural regeneration by accelerating or improving peripheral nerves neoformation. The adipose tissue is abundant in the human body, and it has presented promising results in the regeneration of peripheral nerves. We carried out a randomized controlled study in 9 months, using 45 male Wistar rats, 80 days old, and the sciatic nerve was chosen for analysis. The control animals were divided into three groups – Initial group (IG), Final group (FG), and denervated group (DG) – with seven animals each. The experimental groups, with twelve animals each, were polyethylene tube filled with fat (EGF) and polyethylene tube without filling (EGwf). All groups, except IG, were submitted to 10 sessions of hyperbaric oxygen treatment of 1h 45 min in alternating days. Functional evaluation by walking-track was assessed using the Catwalk XT® software and tissues were harvested and stained with 1% toluidine blue for histological analysis. Quantitative data were first analyzed with the Kolmogorov Smirnov normality test. Comparison between the four groups was analyzed by ANOVA followed by Tukey Test. We concluded that hyperbaric oxygen therapy had positive results on morphometric and functional parameters. However, no significant differences were found regarding the use of autologous fat graft.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2021.vol35.0138
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.35 2021
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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