Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307 |
Resumo: | Abstract The loss of continuity of the nerve structure interrupts the transmission of nerve impulses and leads to the disorganization of functional activities. Many methods, as the use of neurogenic factors, aid in the process of neural regeneration by accelerating or improving peripheral nerves neoformation. The adipose tissue is abundant in the human body, and it has presented promising results in the regeneration of peripheral nerves. We carried out a randomized controlled study in 9 months, using 45 male Wistar rats, 80 days old, and the sciatic nerve was chosen for analysis. The control animals were divided into three groups – Initial group (IG), Final group (FG), and denervated group (DG) – with seven animals each. The experimental groups, with twelve animals each, were polyethylene tube filled with fat (EGF) and polyethylene tube without filling (EGwf). All groups, except IG, were submitted to 10 sessions of hyperbaric oxygen treatment of 1h 45 min in alternating days. Functional evaluation by walking-track was assessed using the Catwalk XT® software and tissues were harvested and stained with 1% toluidine blue for histological analysis. Quantitative data were first analyzed with the Kolmogorov Smirnov normality test. Comparison between the four groups was analyzed by ANOVA followed by Tukey Test. We concluded that hyperbaric oxygen therapy had positive results on morphometric and functional parameters. However, no significant differences were found regarding the use of autologous fat graft. |
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Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapyNerve RegenerationNeuronsNervous SystemNeuronal PlasticityPeripheral NervesAbstract The loss of continuity of the nerve structure interrupts the transmission of nerve impulses and leads to the disorganization of functional activities. Many methods, as the use of neurogenic factors, aid in the process of neural regeneration by accelerating or improving peripheral nerves neoformation. The adipose tissue is abundant in the human body, and it has presented promising results in the regeneration of peripheral nerves. We carried out a randomized controlled study in 9 months, using 45 male Wistar rats, 80 days old, and the sciatic nerve was chosen for analysis. The control animals were divided into three groups – Initial group (IG), Final group (FG), and denervated group (DG) – with seven animals each. The experimental groups, with twelve animals each, were polyethylene tube filled with fat (EGF) and polyethylene tube without filling (EGwf). All groups, except IG, were submitted to 10 sessions of hyperbaric oxygen treatment of 1h 45 min in alternating days. Functional evaluation by walking-track was assessed using the Catwalk XT® software and tissues were harvested and stained with 1% toluidine blue for histological analysis. Quantitative data were first analyzed with the Kolmogorov Smirnov normality test. Comparison between the four groups was analyzed by ANOVA followed by Tukey Test. We concluded that hyperbaric oxygen therapy had positive results on morphometric and functional parameters. However, no significant differences were found regarding the use of autologous fat graft.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307Brazilian Oral Research v.35 2021reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2021.vol35.0138info:eu-repo/semantics/openAccessTOLEDO,Gustavo LopesSANGALETTE,Beatriz SobrinhoPASSEROTTI,Larissa CamargoNASCIMENTO,Juliana de AlmeidaSHINOHARA,André LuisOLIVEIRA,Alexandre Leite Rodrigues deBUZALAF,Marília Afonso RabeloRODRIGUES,Antônio de Castroeng2021-12-15T00:00:00Zoai:scielo:S1806-83242021000100307Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2021-12-15T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
title |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
spellingShingle |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy TOLEDO,Gustavo Lopes Nerve Regeneration Neurons Nervous System Neuronal Plasticity Peripheral Nerves |
title_short |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
title_full |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
title_fullStr |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
title_full_unstemmed |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
title_sort |
Guided neural regeneration with autologous fat grafting and oxygen hyperbaric therapy |
author |
TOLEDO,Gustavo Lopes |
author_facet |
TOLEDO,Gustavo Lopes SANGALETTE,Beatriz Sobrinho PASSEROTTI,Larissa Camargo NASCIMENTO,Juliana de Almeida SHINOHARA,André Luis OLIVEIRA,Alexandre Leite Rodrigues de BUZALAF,Marília Afonso Rabelo RODRIGUES,Antônio de Castro |
author_role |
author |
author2 |
SANGALETTE,Beatriz Sobrinho PASSEROTTI,Larissa Camargo NASCIMENTO,Juliana de Almeida SHINOHARA,André Luis OLIVEIRA,Alexandre Leite Rodrigues de BUZALAF,Marília Afonso Rabelo RODRIGUES,Antônio de Castro |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
TOLEDO,Gustavo Lopes SANGALETTE,Beatriz Sobrinho PASSEROTTI,Larissa Camargo NASCIMENTO,Juliana de Almeida SHINOHARA,André Luis OLIVEIRA,Alexandre Leite Rodrigues de BUZALAF,Marília Afonso Rabelo RODRIGUES,Antônio de Castro |
dc.subject.por.fl_str_mv |
Nerve Regeneration Neurons Nervous System Neuronal Plasticity Peripheral Nerves |
topic |
Nerve Regeneration Neurons Nervous System Neuronal Plasticity Peripheral Nerves |
description |
Abstract The loss of continuity of the nerve structure interrupts the transmission of nerve impulses and leads to the disorganization of functional activities. Many methods, as the use of neurogenic factors, aid in the process of neural regeneration by accelerating or improving peripheral nerves neoformation. The adipose tissue is abundant in the human body, and it has presented promising results in the regeneration of peripheral nerves. We carried out a randomized controlled study in 9 months, using 45 male Wistar rats, 80 days old, and the sciatic nerve was chosen for analysis. The control animals were divided into three groups – Initial group (IG), Final group (FG), and denervated group (DG) – with seven animals each. The experimental groups, with twelve animals each, were polyethylene tube filled with fat (EGF) and polyethylene tube without filling (EGwf). All groups, except IG, were submitted to 10 sessions of hyperbaric oxygen treatment of 1h 45 min in alternating days. Functional evaluation by walking-track was assessed using the Catwalk XT® software and tissues were harvested and stained with 1% toluidine blue for histological analysis. Quantitative data were first analyzed with the Kolmogorov Smirnov normality test. Comparison between the four groups was analyzed by ANOVA followed by Tukey Test. We concluded that hyperbaric oxygen therapy had positive results on morphometric and functional parameters. However, no significant differences were found regarding the use of autologous fat graft. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100307 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2021.vol35.0138 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.35 2021 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318328011816960 |