Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100248 |
Resumo: | Abstract This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student’s t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials. |
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Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®EndodonticsMaterials TestingHypertensionAbstract This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student’s t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100248Brazilian Oral Research v.33 2019reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2019.vol33.0060info:eu-repo/semantics/openAccessCOSME-SILVA,LeopoldoDAL-FABBRO,RenanGONÇALVES,Luanna de OliveiraPRADO,Alana Sant’Ana doPLAZZA,Flávia AlfredoVIOLA,Naiana VianaCINTRA,Luciano Tavares AngeloGOMES FILHO,João Eduardoeng2019-07-24T00:00:00Zoai:scielo:S1806-83242019000100248Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2019-07-24T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
spellingShingle |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® COSME-SILVA,Leopoldo Endodontics Materials Testing Hypertension |
title_short |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_full |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_fullStr |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_full_unstemmed |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_sort |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
author |
COSME-SILVA,Leopoldo |
author_facet |
COSME-SILVA,Leopoldo DAL-FABBRO,Renan GONÇALVES,Luanna de Oliveira PRADO,Alana Sant’Ana do PLAZZA,Flávia Alfredo VIOLA,Naiana Viana CINTRA,Luciano Tavares Angelo GOMES FILHO,João Eduardo |
author_role |
author |
author2 |
DAL-FABBRO,Renan GONÇALVES,Luanna de Oliveira PRADO,Alana Sant’Ana do PLAZZA,Flávia Alfredo VIOLA,Naiana Viana CINTRA,Luciano Tavares Angelo GOMES FILHO,João Eduardo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
COSME-SILVA,Leopoldo DAL-FABBRO,Renan GONÇALVES,Luanna de Oliveira PRADO,Alana Sant’Ana do PLAZZA,Flávia Alfredo VIOLA,Naiana Viana CINTRA,Luciano Tavares Angelo GOMES FILHO,João Eduardo |
dc.subject.por.fl_str_mv |
Endodontics Materials Testing Hypertension |
topic |
Endodontics Materials Testing Hypertension |
description |
Abstract This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student’s t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100248 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100248 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2019.vol33.0060 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.33 2019 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318326574219264 |