Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008 |
Resumo: | Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor. |
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Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinomaCarcinomaSquamous CellVimentinExtracellular MatrixVimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008Brazilian Oral Research v.25 n.3 2011reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242011000300008info:eu-repo/semantics/openAccessVechio,Aluana Maria da Costa DalGiudice,Fernanda SalgueiredoSperandio,Felipe ForniasMantesso,AndreaPinto Junior,Décio dos Santoseng2011-06-03T00:00:00Zoai:scielo:S1806-83242011000300008Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2011-06-03T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
title |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
spellingShingle |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma Vechio,Aluana Maria da Costa Dal Carcinoma Squamous Cell Vimentin Extracellular Matrix |
title_short |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
title_full |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
title_fullStr |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
title_full_unstemmed |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
title_sort |
Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma |
author |
Vechio,Aluana Maria da Costa Dal |
author_facet |
Vechio,Aluana Maria da Costa Dal Giudice,Fernanda Salgueiredo Sperandio,Felipe Fornias Mantesso,Andrea Pinto Junior,Décio dos Santos |
author_role |
author |
author2 |
Giudice,Fernanda Salgueiredo Sperandio,Felipe Fornias Mantesso,Andrea Pinto Junior,Décio dos Santos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Vechio,Aluana Maria da Costa Dal Giudice,Fernanda Salgueiredo Sperandio,Felipe Fornias Mantesso,Andrea Pinto Junior,Décio dos Santos |
dc.subject.por.fl_str_mv |
Carcinoma Squamous Cell Vimentin Extracellular Matrix |
topic |
Carcinoma Squamous Cell Vimentin Extracellular Matrix |
description |
Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242011000300008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.25 n.3 2011 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318322177540096 |