Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma

Detalhes bibliográficos
Autor(a) principal: Vechio,Aluana Maria da Costa Dal
Data de Publicação: 2011
Outros Autores: Giudice,Fernanda Salgueiredo, Sperandio,Felipe Fornias, Mantesso,Andrea, Pinto Junior,Décio dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008
Resumo: Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor.
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spelling Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinomaCarcinomaSquamous CellVimentinExtracellular MatrixVimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008Brazilian Oral Research v.25 n.3 2011reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242011000300008info:eu-repo/semantics/openAccessVechio,Aluana Maria da Costa DalGiudice,Fernanda SalgueiredoSperandio,Felipe ForniasMantesso,AndreaPinto Junior,Décio dos Santoseng2011-06-03T00:00:00Zoai:scielo:S1806-83242011000300008Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2011-06-03T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
title Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
spellingShingle Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
Vechio,Aluana Maria da Costa Dal
Carcinoma
Squamous Cell
Vimentin
Extracellular Matrix
title_short Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
title_full Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
title_fullStr Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
title_full_unstemmed Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
title_sort Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
author Vechio,Aluana Maria da Costa Dal
author_facet Vechio,Aluana Maria da Costa Dal
Giudice,Fernanda Salgueiredo
Sperandio,Felipe Fornias
Mantesso,Andrea
Pinto Junior,Décio dos Santos
author_role author
author2 Giudice,Fernanda Salgueiredo
Sperandio,Felipe Fornias
Mantesso,Andrea
Pinto Junior,Décio dos Santos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vechio,Aluana Maria da Costa Dal
Giudice,Fernanda Salgueiredo
Sperandio,Felipe Fornias
Mantesso,Andrea
Pinto Junior,Décio dos Santos
dc.subject.por.fl_str_mv Carcinoma
Squamous Cell
Vimentin
Extracellular Matrix
topic Carcinoma
Squamous Cell
Vimentin
Extracellular Matrix
description Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242011000300008
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/S1806-83242011000300008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.25 n.3 2011
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
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reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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