Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma

Detalhes bibliográficos
Autor(a) principal: COSTA,Liana Cristina Melo Carneiro
Data de Publicação: 2015
Outros Autores: LEITE,Camila Ferreira, CARDOSO,Sérgio Vitorino, LOYOLA,Adriano Mota, FARIA,Paulo Rogério de, SOUZA,Paulo Eduardo Alencar, HORTA,Martinho Campolina Rebello
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000200010
Resumo: Oral squamous cell carcinoma (OSCC) is one of the most common malignances. In epithelial-mesenchymal transition (EMT), epithelial cells switch to mesenchymal-like cells exhibiting high mobility. This migratory phenotype is significant during tumor invasion and metastasis.Objective: The aim of this study is to evaluate the expression of the EMT markers E-cadherin, N-cadherin and vimentin in OSCC.Material and Methods : Immunohistochemical detection of E-cadherin, N-cadherin and vimentin was performed on 20 OSCC samples. Differences in the expression of each protein at the invasive front (IF) and in the central/superficial areas (CSA) of the tumor were assessed. Differences in the expression of each protein at the IF of both histologically high- and low-invasive OSCCs were evaluated. Associations among expression of proteins at the IF were assessed. Correlations between the expression levels of each protein at the IF and the tumor stage and clinical nodal status were also evaluated.Results : Reduced expression of E-cadherin was detected in 15 samples (75%). E-cadherin expression was reduced at the IF when compared to the CSA and in high-invasive tumors when compared to low-invasive tumors. All samples were negative for N-cadherin, even though one sample showed an inconspicuous expression. Positive expression of vimentin was observed in 6 samples (30%). Nevertheless, there was no difference in vimentin expression between the IF and the CSA regions or between the low- and high-invasive tumors. Furthermore, no association was observed among protein expression levels at the IF. Finally, no correlations were observed between each protein’s expression levels and tumor stage or clinical nodal status.Conclusions : Reduced E-cadherin expression at the IF and its association with histological invasiveness suggest that this protein is a noteworthy EMT marker in OSCC. Although vimentin was also detected as an EMT marker, its expression was neither limited to the IF nor was it related to histological invasiveness.
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spelling Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinomaOral cancerSquamous cell carcinomaCadherinsVimentinOral squamous cell carcinoma (OSCC) is one of the most common malignances. In epithelial-mesenchymal transition (EMT), epithelial cells switch to mesenchymal-like cells exhibiting high mobility. This migratory phenotype is significant during tumor invasion and metastasis.Objective: The aim of this study is to evaluate the expression of the EMT markers E-cadherin, N-cadherin and vimentin in OSCC.Material and Methods : Immunohistochemical detection of E-cadherin, N-cadherin and vimentin was performed on 20 OSCC samples. Differences in the expression of each protein at the invasive front (IF) and in the central/superficial areas (CSA) of the tumor were assessed. Differences in the expression of each protein at the IF of both histologically high- and low-invasive OSCCs were evaluated. Associations among expression of proteins at the IF were assessed. Correlations between the expression levels of each protein at the IF and the tumor stage and clinical nodal status were also evaluated.Results : Reduced expression of E-cadherin was detected in 15 samples (75%). E-cadherin expression was reduced at the IF when compared to the CSA and in high-invasive tumors when compared to low-invasive tumors. All samples were negative for N-cadherin, even though one sample showed an inconspicuous expression. Positive expression of vimentin was observed in 6 samples (30%). Nevertheless, there was no difference in vimentin expression between the IF and the CSA regions or between the low- and high-invasive tumors. Furthermore, no association was observed among protein expression levels at the IF. Finally, no correlations were observed between each protein’s expression levels and tumor stage or clinical nodal status.Conclusions : Reduced E-cadherin expression at the IF and its association with histological invasiveness suggest that this protein is a noteworthy EMT marker in OSCC. Although vimentin was also detected as an EMT marker, its expression was neither limited to the IF nor was it related to histological invasiveness.Faculdade De Odontologia De Bauru - USP2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000200010Journal of Applied Oral Science v.23 n.2 2015reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720140187info:eu-repo/semantics/openAccessCOSTA,Liana Cristina Melo CarneiroLEITE,Camila FerreiraCARDOSO,Sérgio VitorinoLOYOLA,Adriano MotaFARIA,Paulo Rogério deSOUZA,Paulo Eduardo AlencarHORTA,Martinho Campolina Rebelloeng2015-10-14T00:00:00Zoai:scielo:S1678-77572015000200010Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2015-10-14T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
title Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
spellingShingle Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
COSTA,Liana Cristina Melo Carneiro
Oral cancer
Squamous cell carcinoma
Cadherins
Vimentin
title_short Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
title_full Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
title_fullStr Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
title_full_unstemmed Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
title_sort Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
author COSTA,Liana Cristina Melo Carneiro
author_facet COSTA,Liana Cristina Melo Carneiro
LEITE,Camila Ferreira
CARDOSO,Sérgio Vitorino
LOYOLA,Adriano Mota
FARIA,Paulo Rogério de
SOUZA,Paulo Eduardo Alencar
HORTA,Martinho Campolina Rebello
author_role author
author2 LEITE,Camila Ferreira
CARDOSO,Sérgio Vitorino
LOYOLA,Adriano Mota
FARIA,Paulo Rogério de
SOUZA,Paulo Eduardo Alencar
HORTA,Martinho Campolina Rebello
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv COSTA,Liana Cristina Melo Carneiro
LEITE,Camila Ferreira
CARDOSO,Sérgio Vitorino
LOYOLA,Adriano Mota
FARIA,Paulo Rogério de
SOUZA,Paulo Eduardo Alencar
HORTA,Martinho Campolina Rebello
dc.subject.por.fl_str_mv Oral cancer
Squamous cell carcinoma
Cadherins
Vimentin
topic Oral cancer
Squamous cell carcinoma
Cadherins
Vimentin
description Oral squamous cell carcinoma (OSCC) is one of the most common malignances. In epithelial-mesenchymal transition (EMT), epithelial cells switch to mesenchymal-like cells exhibiting high mobility. This migratory phenotype is significant during tumor invasion and metastasis.Objective: The aim of this study is to evaluate the expression of the EMT markers E-cadherin, N-cadherin and vimentin in OSCC.Material and Methods : Immunohistochemical detection of E-cadherin, N-cadherin and vimentin was performed on 20 OSCC samples. Differences in the expression of each protein at the invasive front (IF) and in the central/superficial areas (CSA) of the tumor were assessed. Differences in the expression of each protein at the IF of both histologically high- and low-invasive OSCCs were evaluated. Associations among expression of proteins at the IF were assessed. Correlations between the expression levels of each protein at the IF and the tumor stage and clinical nodal status were also evaluated.Results : Reduced expression of E-cadherin was detected in 15 samples (75%). E-cadherin expression was reduced at the IF when compared to the CSA and in high-invasive tumors when compared to low-invasive tumors. All samples were negative for N-cadherin, even though one sample showed an inconspicuous expression. Positive expression of vimentin was observed in 6 samples (30%). Nevertheless, there was no difference in vimentin expression between the IF and the CSA regions or between the low- and high-invasive tumors. Furthermore, no association was observed among protein expression levels at the IF. Finally, no correlations were observed between each protein’s expression levels and tumor stage or clinical nodal status.Conclusions : Reduced E-cadherin expression at the IF and its association with histological invasiveness suggest that this protein is a noteworthy EMT marker in OSCC. Although vimentin was also detected as an EMT marker, its expression was neither limited to the IF nor was it related to histological invasiveness.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000200010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000200010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-775720140187
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.23 n.2 2015
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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