Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100272 |
Resumo: | The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups: χ2: p < 0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification ofCandida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence ofCandida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candidaspp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque. |
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Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabeticsDiabetes Mellitus, Type 2Chronic PeriodontitisCandidaPeriodontal PocketThe purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups: χ2: p < 0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification ofCandida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence ofCandida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candidaspp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100272Brazilian Oral Research v.29 n.1 2015reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2015.vol29.0077info:eu-repo/semantics/openAccessMATIĆ PETROVIĆ,SanjaCIMBALJEVIĆ,MilenaRADUNOVIĆ,MilenaKUZMANOVIĆ PFIĆER,JovanaJOTIĆ,AleksandraPUCAR,Anaeng2018-09-17T00:00:00Zoai:scielo:S1806-83242015000100272Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-09-17T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
title |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
spellingShingle |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics MATIĆ PETROVIĆ,Sanja Diabetes Mellitus, Type 2 Chronic Periodontitis Candida Periodontal Pocket |
title_short |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
title_full |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
title_fullStr |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
title_full_unstemmed |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
title_sort |
Detection and sampling methods for isolation of Candidaspp. from oral cavities in diabetics and non-diabetics |
author |
MATIĆ PETROVIĆ,Sanja |
author_facet |
MATIĆ PETROVIĆ,Sanja CIMBALJEVIĆ,Milena RADUNOVIĆ,Milena KUZMANOVIĆ PFIĆER,Jovana JOTIĆ,Aleksandra PUCAR,Ana |
author_role |
author |
author2 |
CIMBALJEVIĆ,Milena RADUNOVIĆ,Milena KUZMANOVIĆ PFIĆER,Jovana JOTIĆ,Aleksandra PUCAR,Ana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
MATIĆ PETROVIĆ,Sanja CIMBALJEVIĆ,Milena RADUNOVIĆ,Milena KUZMANOVIĆ PFIĆER,Jovana JOTIĆ,Aleksandra PUCAR,Ana |
dc.subject.por.fl_str_mv |
Diabetes Mellitus, Type 2 Chronic Periodontitis Candida Periodontal Pocket |
topic |
Diabetes Mellitus, Type 2 Chronic Periodontitis Candida Periodontal Pocket |
description |
The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups: χ2: p < 0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification ofCandida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence ofCandida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candidaspp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100272 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100272 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107BOR-2015.vol29.0077 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.29 n.1 2015 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318324288323584 |