Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep

Detalhes bibliográficos
Autor(a) principal: MACHADO,Callinca Paolla Gomes
Data de Publicação: 2016
Outros Autores: SARTORETTO,Suelen Cristina, ALVES,Adriana Terezinha Neves Novellino, LIMA,Inayá Barbosa Correa, ROSSI,Alexandre Malta, GRANJEIRO,José Mauro, CALASANS-MAIA,Mônica Diuana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243
Resumo: Abstract The aim of this study is to evaluate the biocompatibility and osteoconductivity in surgical defects of sheep tibias filled with 1% strontium-containing nanostructured hydroxyapatite microspheres (SrHA), stoichiometric hydroxyapatite without strontium microspheres (HA), or blood clots. Santa Ines sheep were subjected to three perforations on the medial side of the left tibia. The biomaterials were characterized by X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) before implantation and by X-Ray Microfluorescence (µFRX) and Scanning Electron Microscopy (SEM) after sheep tibias implantation. Surgical defects were filled with blood clots (control), SrHA (Group 1) or HA (Group 2). After 30 days, 5-µm bone blocks were obtained for histological evaluation, and the blocks obtained from 1 animal were embedded in methylmethacrylate for undecalcified sections. Mononuclear inflammatory infiltrate remained mild in all experimental groups. Giant cells were observed surrounding biomaterials particles of both groups and areas of bone formation were detected in close contact with biomaterials. All groups showed newly formed bone from the periphery to the center of the defects, which the control, HA and SrHA presented 36.4% (± 21.8), 31.2% (± 14.7) and 26.2% (± 12.9) of newly formed bone density, respectively, not presenting statistical differences. In addition, the connective tissue density did not show any significant between groups. The SrHA showing a higher volume density of biomaterial (51.2 ± 14.1) present in the defect compared to HA (32.6 ± 8.5) after 30 days (p = 0.03). Microspheres containing 1% SrHA or HA can be considered biocompatible, have osteoconductive properties and may be useful biomaterials for clinical applications.
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spelling Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheepBone RegenerationHistologyModelsAnimalAbstract The aim of this study is to evaluate the biocompatibility and osteoconductivity in surgical defects of sheep tibias filled with 1% strontium-containing nanostructured hydroxyapatite microspheres (SrHA), stoichiometric hydroxyapatite without strontium microspheres (HA), or blood clots. Santa Ines sheep were subjected to three perforations on the medial side of the left tibia. The biomaterials were characterized by X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) before implantation and by X-Ray Microfluorescence (µFRX) and Scanning Electron Microscopy (SEM) after sheep tibias implantation. Surgical defects were filled with blood clots (control), SrHA (Group 1) or HA (Group 2). After 30 days, 5-µm bone blocks were obtained for histological evaluation, and the blocks obtained from 1 animal were embedded in methylmethacrylate for undecalcified sections. Mononuclear inflammatory infiltrate remained mild in all experimental groups. Giant cells were observed surrounding biomaterials particles of both groups and areas of bone formation were detected in close contact with biomaterials. All groups showed newly formed bone from the periphery to the center of the defects, which the control, HA and SrHA presented 36.4% (± 21.8), 31.2% (± 14.7) and 26.2% (± 12.9) of newly formed bone density, respectively, not presenting statistical differences. In addition, the connective tissue density did not show any significant between groups. The SrHA showing a higher volume density of biomaterial (51.2 ± 14.1) present in the defect compared to HA (32.6 ± 8.5) after 30 days (p = 0.03). Microspheres containing 1% SrHA or HA can be considered biocompatible, have osteoconductive properties and may be useful biomaterials for clinical applications.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243Brazilian Oral Research v.30 n.1 2016reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2016.vol30.0045info:eu-repo/semantics/openAccessMACHADO,Callinca Paolla GomesSARTORETTO,Suelen CristinaALVES,Adriana Terezinha Neves NovellinoLIMA,Inayá Barbosa CorreaROSSI,Alexandre MaltaGRANJEIRO,José MauroCALASANS-MAIA,Mônica Diuanaeng2016-05-11T00:00:00Zoai:scielo:S1806-83242016000100243Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2016-05-11T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
title Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
spellingShingle Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
MACHADO,Callinca Paolla Gomes
Bone Regeneration
Histology
Models
Animal
title_short Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
title_full Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
title_fullStr Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
title_full_unstemmed Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
title_sort Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
author MACHADO,Callinca Paolla Gomes
author_facet MACHADO,Callinca Paolla Gomes
SARTORETTO,Suelen Cristina
ALVES,Adriana Terezinha Neves Novellino
LIMA,Inayá Barbosa Correa
ROSSI,Alexandre Malta
GRANJEIRO,José Mauro
CALASANS-MAIA,Mônica Diuana
author_role author
author2 SARTORETTO,Suelen Cristina
ALVES,Adriana Terezinha Neves Novellino
LIMA,Inayá Barbosa Correa
ROSSI,Alexandre Malta
GRANJEIRO,José Mauro
CALASANS-MAIA,Mônica Diuana
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv MACHADO,Callinca Paolla Gomes
SARTORETTO,Suelen Cristina
ALVES,Adriana Terezinha Neves Novellino
LIMA,Inayá Barbosa Correa
ROSSI,Alexandre Malta
GRANJEIRO,José Mauro
CALASANS-MAIA,Mônica Diuana
dc.subject.por.fl_str_mv Bone Regeneration
Histology
Models
Animal
topic Bone Regeneration
Histology
Models
Animal
description Abstract The aim of this study is to evaluate the biocompatibility and osteoconductivity in surgical defects of sheep tibias filled with 1% strontium-containing nanostructured hydroxyapatite microspheres (SrHA), stoichiometric hydroxyapatite without strontium microspheres (HA), or blood clots. Santa Ines sheep were subjected to three perforations on the medial side of the left tibia. The biomaterials were characterized by X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) before implantation and by X-Ray Microfluorescence (µFRX) and Scanning Electron Microscopy (SEM) after sheep tibias implantation. Surgical defects were filled with blood clots (control), SrHA (Group 1) or HA (Group 2). After 30 days, 5-µm bone blocks were obtained for histological evaluation, and the blocks obtained from 1 animal were embedded in methylmethacrylate for undecalcified sections. Mononuclear inflammatory infiltrate remained mild in all experimental groups. Giant cells were observed surrounding biomaterials particles of both groups and areas of bone formation were detected in close contact with biomaterials. All groups showed newly formed bone from the periphery to the center of the defects, which the control, HA and SrHA presented 36.4% (± 21.8), 31.2% (± 14.7) and 26.2% (± 12.9) of newly formed bone density, respectively, not presenting statistical differences. In addition, the connective tissue density did not show any significant between groups. The SrHA showing a higher volume density of biomaterial (51.2 ± 14.1) present in the defect compared to HA (32.6 ± 8.5) after 30 days (p = 0.03). Microspheres containing 1% SrHA or HA can be considered biocompatible, have osteoconductive properties and may be useful biomaterials for clinical applications.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107BOR-2016.vol30.0045
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.30 n.1 2016
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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