Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243 |
Resumo: | Abstract The aim of this study is to evaluate the biocompatibility and osteoconductivity in surgical defects of sheep tibias filled with 1% strontium-containing nanostructured hydroxyapatite microspheres (SrHA), stoichiometric hydroxyapatite without strontium microspheres (HA), or blood clots. Santa Ines sheep were subjected to three perforations on the medial side of the left tibia. The biomaterials were characterized by X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) before implantation and by X-Ray Microfluorescence (µFRX) and Scanning Electron Microscopy (SEM) after sheep tibias implantation. Surgical defects were filled with blood clots (control), SrHA (Group 1) or HA (Group 2). After 30 days, 5-µm bone blocks were obtained for histological evaluation, and the blocks obtained from 1 animal were embedded in methylmethacrylate for undecalcified sections. Mononuclear inflammatory infiltrate remained mild in all experimental groups. Giant cells were observed surrounding biomaterials particles of both groups and areas of bone formation were detected in close contact with biomaterials. All groups showed newly formed bone from the periphery to the center of the defects, which the control, HA and SrHA presented 36.4% (± 21.8), 31.2% (± 14.7) and 26.2% (± 12.9) of newly formed bone density, respectively, not presenting statistical differences. In addition, the connective tissue density did not show any significant between groups. The SrHA showing a higher volume density of biomaterial (51.2 ± 14.1) present in the defect compared to HA (32.6 ± 8.5) after 30 days (p = 0.03). Microspheres containing 1% SrHA or HA can be considered biocompatible, have osteoconductive properties and may be useful biomaterials for clinical applications. |
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Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheepBone RegenerationHistologyModelsAnimalAbstract The aim of this study is to evaluate the biocompatibility and osteoconductivity in surgical defects of sheep tibias filled with 1% strontium-containing nanostructured hydroxyapatite microspheres (SrHA), stoichiometric hydroxyapatite without strontium microspheres (HA), or blood clots. Santa Ines sheep were subjected to three perforations on the medial side of the left tibia. The biomaterials were characterized by X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) before implantation and by X-Ray Microfluorescence (µFRX) and Scanning Electron Microscopy (SEM) after sheep tibias implantation. Surgical defects were filled with blood clots (control), SrHA (Group 1) or HA (Group 2). After 30 days, 5-µm bone blocks were obtained for histological evaluation, and the blocks obtained from 1 animal were embedded in methylmethacrylate for undecalcified sections. Mononuclear inflammatory infiltrate remained mild in all experimental groups. Giant cells were observed surrounding biomaterials particles of both groups and areas of bone formation were detected in close contact with biomaterials. All groups showed newly formed bone from the periphery to the center of the defects, which the control, HA and SrHA presented 36.4% (± 21.8), 31.2% (± 14.7) and 26.2% (± 12.9) of newly formed bone density, respectively, not presenting statistical differences. In addition, the connective tissue density did not show any significant between groups. The SrHA showing a higher volume density of biomaterial (51.2 ± 14.1) present in the defect compared to HA (32.6 ± 8.5) after 30 days (p = 0.03). Microspheres containing 1% SrHA or HA can be considered biocompatible, have osteoconductive properties and may be useful biomaterials for clinical applications.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243Brazilian Oral Research v.30 n.1 2016reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2016.vol30.0045info:eu-repo/semantics/openAccessMACHADO,Callinca Paolla GomesSARTORETTO,Suelen CristinaALVES,Adriana Terezinha Neves NovellinoLIMA,Inayá Barbosa CorreaROSSI,Alexandre MaltaGRANJEIRO,José MauroCALASANS-MAIA,Mônica Diuanaeng2016-05-11T00:00:00Zoai:scielo:S1806-83242016000100243Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2016-05-11T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
title |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
spellingShingle |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep MACHADO,Callinca Paolla Gomes Bone Regeneration Histology Models Animal |
title_short |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
title_full |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
title_fullStr |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
title_full_unstemmed |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
title_sort |
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep |
author |
MACHADO,Callinca Paolla Gomes |
author_facet |
MACHADO,Callinca Paolla Gomes SARTORETTO,Suelen Cristina ALVES,Adriana Terezinha Neves Novellino LIMA,Inayá Barbosa Correa ROSSI,Alexandre Malta GRANJEIRO,José Mauro CALASANS-MAIA,Mônica Diuana |
author_role |
author |
author2 |
SARTORETTO,Suelen Cristina ALVES,Adriana Terezinha Neves Novellino LIMA,Inayá Barbosa Correa ROSSI,Alexandre Malta GRANJEIRO,José Mauro CALASANS-MAIA,Mônica Diuana |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
MACHADO,Callinca Paolla Gomes SARTORETTO,Suelen Cristina ALVES,Adriana Terezinha Neves Novellino LIMA,Inayá Barbosa Correa ROSSI,Alexandre Malta GRANJEIRO,José Mauro CALASANS-MAIA,Mônica Diuana |
dc.subject.por.fl_str_mv |
Bone Regeneration Histology Models Animal |
topic |
Bone Regeneration Histology Models Animal |
description |
Abstract The aim of this study is to evaluate the biocompatibility and osteoconductivity in surgical defects of sheep tibias filled with 1% strontium-containing nanostructured hydroxyapatite microspheres (SrHA), stoichiometric hydroxyapatite without strontium microspheres (HA), or blood clots. Santa Ines sheep were subjected to three perforations on the medial side of the left tibia. The biomaterials were characterized by X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) before implantation and by X-Ray Microfluorescence (µFRX) and Scanning Electron Microscopy (SEM) after sheep tibias implantation. Surgical defects were filled with blood clots (control), SrHA (Group 1) or HA (Group 2). After 30 days, 5-µm bone blocks were obtained for histological evaluation, and the blocks obtained from 1 animal were embedded in methylmethacrylate for undecalcified sections. Mononuclear inflammatory infiltrate remained mild in all experimental groups. Giant cells were observed surrounding biomaterials particles of both groups and areas of bone formation were detected in close contact with biomaterials. All groups showed newly formed bone from the periphery to the center of the defects, which the control, HA and SrHA presented 36.4% (± 21.8), 31.2% (± 14.7) and 26.2% (± 12.9) of newly formed bone density, respectively, not presenting statistical differences. In addition, the connective tissue density did not show any significant between groups. The SrHA showing a higher volume density of biomaterial (51.2 ± 14.1) present in the defect compared to HA (32.6 ± 8.5) after 30 days (p = 0.03). Microspheres containing 1% SrHA or HA can be considered biocompatible, have osteoconductive properties and may be useful biomaterials for clinical applications. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100243 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107BOR-2016.vol30.0045 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.30 n.1 2016 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318324727676928 |