Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Iorrana Índira dos Anjos
Data de Publicação: 2023
Outros Autores: Barbosa Junior, Aryon de Almeida, Rossi, Alexandre Malta, Almeida, Renata dos Santos, Miguel, Fúlvio Borges, Rosa, Fabiana Paim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/41222
Resumo: The aim of this study was to analyze the biological behavior and osteogenic potential of nanostructured hydroxyapatite microspheres substituted with strontium (nHASr). Therefore, twenty adult male Wistar rats were randomly distributed into two groups: GnHASr – critical bone defect filled with nHASr microspheres; e CG (control group) – critical bone defect without implantation of biomaterial; evaluated at the biological points of 30 and 60 days. The collected specimens were processed and stained with hematoxylin-eosin (HE) and Masson-Goldner trichrome (TG) and examined by light microscopy. Posteriorly, they were analyzed histomorphometrically to measure the percentage of neoformed osteoid matrix (%OM). In both groups studied, at all biological points, deposition of reparative osteoid matrix (OM) was observed near the bone edges; discrete chronic inflammatory response; connective tissue formation and neovascularization in the residual area of the defect. In the GnHASr, in the two evaluated periods, the deposition of OM was also noticed, both around and inside the microspheres. At 60 days, an area of 7.54% of OM deposition in relation to the total defect area was evidenced in the GnHASr, while in the CG this value was 6.80%. It is concluded that the nHASr microspheres evaluated were biocompatible, biodegradable, bioresorbable, bioactive and osteoconductive. In both groups, the formation of neomineralized tissue occurred in a limited way, which indicates that the concentration of metal used in the replacement did not favor greater osteogenic potential for the biomaterial. The evaluated biomaterial it's adequate for use as a filling material.
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spelling Strontium-containing nanostructured hydroxyapatite microspheres for bone regenerationMicroesferas de hidroxiapatita nanoestructuradas sustituidas con estroncio para la regeneración óseaMicroesferas de hidroxiapatita nanoestruturada substituída por estrôncio para regeneração ósseaBiomaterialsBone regenerationCritical bone defectHydroxyapatiteStrontium.BiomaterialesRegeneración óseaDefecto óseo críticoHidroxiapatitaEstroncio.BiomateriaisRegeneração ÓsseaDefeito ósseo críticoHidroxiapatitaEstrôncio.The aim of this study was to analyze the biological behavior and osteogenic potential of nanostructured hydroxyapatite microspheres substituted with strontium (nHASr). Therefore, twenty adult male Wistar rats were randomly distributed into two groups: GnHASr – critical bone defect filled with nHASr microspheres; e CG (control group) – critical bone defect without implantation of biomaterial; evaluated at the biological points of 30 and 60 days. The collected specimens were processed and stained with hematoxylin-eosin (HE) and Masson-Goldner trichrome (TG) and examined by light microscopy. Posteriorly, they were analyzed histomorphometrically to measure the percentage of neoformed osteoid matrix (%OM). In both groups studied, at all biological points, deposition of reparative osteoid matrix (OM) was observed near the bone edges; discrete chronic inflammatory response; connective tissue formation and neovascularization in the residual area of the defect. In the GnHASr, in the two evaluated periods, the deposition of OM was also noticed, both around and inside the microspheres. At 60 days, an area of 7.54% of OM deposition in relation to the total defect area was evidenced in the GnHASr, while in the CG this value was 6.80%. It is concluded that the nHASr microspheres evaluated were biocompatible, biodegradable, bioresorbable, bioactive and osteoconductive. In both groups, the formation of neomineralized tissue occurred in a limited way, which indicates that the concentration of metal used in the replacement did not favor greater osteogenic potential for the biomaterial. The evaluated biomaterial it's adequate for use as a filling material.El objetivo de este estudio fue analizar el comportamiento biológico y el potencial osteogénico de microesferas de hidroxiapatita nanoestructuradas sustituidas con estrôncio (nHASr). Para tanto, veinte ratas wistar macho adultas se distribuyeron aleatoriamente en dos grupos: GnHASr – defecto óseo crítico relleno con microesferas nHASr; e GC (grupo de control) - defecto óseo crítico sin implantación de biomaterial; evaluado en los puntos biológicos de 30 y 60 días. Los especímenes recolectados fueron processados y teñidos con hematoxilina-eosina (HE) y tricrómico de Masson-Goldner (TG), y examinados por microscopía de luz común. Posteriormente, se analizaron histomorfométricamente para medir el porcentaje de matriz osteoide neoformada (%MO). En ambos grupos estudiados, en todos los puntos biológicos se observó depósito de matriz osteoide (MO) reparadora, cerca de los bordes óseos; respuesta inflamatoria crónica leve; formación de tejido conectivo y neovascularización en el área residual del defecto. En el GnHASr, en los dos periodos evaluados, también se notó la deposición de MO, tanto alrededor como dentro de las microesferas. A los 60 días se evidenció en el GnHASr un área de 7,54% de depósito de MO con relación al área total del defecto, mientras que en el GC este valor fue de 6,80%. Se concluye que las microesferas de nHASr evaluadas en este estudio fueron biocompatibles, biodegradables, biorreabsorbibles, bioactivas y osteoconductoras. En ambos grupos, la formación de tejido neomineralizado ocurrió de forma limitada, lo que indica que la concentración de metal utilizada en el reemplazo no favoreció un mayor potencial osteogénico para el biomaterial. El biomaterial evaluado es adecuado para su uso como material de relleno.O objetivo deste estudo foi analisar o comportamento biológico e potencial osteogênico de microesferas de hidroxiapatita nanoestruturadas substituídas com estrôncio (nHASr). Para tanto, utilizou-se vinte ratos wistar, adultos, machos, distribuídos, aleatoriamente, em dois grupos: GnHASr – defeito ósseo crítico preenchido com microesferas de nHASr; e GC (grupo controle) – defeito ósseo crítico sem implantação de biomaterial; avaliados nos pontos biológicos de 30 e 60 dias. Os espécimes foram processados e corados por hematoxilina-eosina (HE) e tricrômico de Masson-Goldner (TG), e examinados por microscopia de luz comum. Posteriormente, foram analisados histomorfometricamente, para mensuração do percentual de matriz osteoide neoformada (%MO). Nos dois grupos estudados, em todos os pontos biológicos, observou-se deposição de matriz osteoide (MO) reparativa, próxima às bordas ósseas; resposta inflamatória crônica discreta; formação de tecido conjuntivo e neovascularização na área residual do defeito. No GnHASr, nos dois períodos avaliados, a deposição de MO foi notada, também, tanto de forma circunjacente quanto no interior das microesferas. Aos 60 dias, evidenciou-se no GnHASr uma área de 7,54% de deposição de MO em relação a área total do defeito, enquanto no GC este valor foi de 6,80%. Conclui-se que as microesferas de nHASr avaliadas neste estudo foram biocompatíveis, biodegradáveis, biorreabsorvíveis, bioativas e osteocondutoras. Nos dois grupos, a formação de tecido neomineralizado ocorreu de forma limitada, isto indica que a concentração de metal utilizada na substituição não favoreceu maior potencial osteogênico ao biomaterial. O biomaterial avaliado é adequado para ser utilizado como material de preenchimento.Research, Society and Development2023-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4122210.33448/rsd-v12i4.41222Research, Society and Development; Vol. 12 No. 4; e22112441222Research, Society and Development; Vol. 12 Núm. 4; e22112441222Research, Society and Development; v. 12 n. 4; e221124412222525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/41222/33546Copyright (c) 2023 Iorrana Índira dos Anjos Ribeiro; Aryon de Almeida Barbosa Junior; Alexandre Malta Rossi; Renata dos Santos Almeida; Fúlvio Borges Miguel; Fabiana Paim Rosahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRibeiro, Iorrana Índira dos Anjos Barbosa Junior, Aryon de Almeida Rossi, Alexandre Malta Almeida, Renata dos SantosMiguel, Fúlvio BorgesRosa, Fabiana Paim2023-04-21T18:13:32Zoai:ojs.pkp.sfu.ca:article/41222Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-04-21T18:13:32Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
Microesferas de hidroxiapatita nanoestructuradas sustituidas con estroncio para la regeneración ósea
Microesferas de hidroxiapatita nanoestruturada substituída por estrôncio para regeneração óssea
title Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
spellingShingle Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
Ribeiro, Iorrana Índira dos Anjos
Biomaterials
Bone regeneration
Critical bone defect
Hydroxyapatite
Strontium.
Biomateriales
Regeneración ósea
Defecto óseo crítico
Hidroxiapatita
Estroncio.
Biomateriais
Regeneração Óssea
Defeito ósseo crítico
Hidroxiapatita
Estrôncio.
title_short Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
title_full Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
title_fullStr Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
title_full_unstemmed Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
title_sort Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
author Ribeiro, Iorrana Índira dos Anjos
author_facet Ribeiro, Iorrana Índira dos Anjos
Barbosa Junior, Aryon de Almeida
Rossi, Alexandre Malta
Almeida, Renata dos Santos
Miguel, Fúlvio Borges
Rosa, Fabiana Paim
author_role author
author2 Barbosa Junior, Aryon de Almeida
Rossi, Alexandre Malta
Almeida, Renata dos Santos
Miguel, Fúlvio Borges
Rosa, Fabiana Paim
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Iorrana Índira dos Anjos
Barbosa Junior, Aryon de Almeida
Rossi, Alexandre Malta
Almeida, Renata dos Santos
Miguel, Fúlvio Borges
Rosa, Fabiana Paim
dc.subject.por.fl_str_mv Biomaterials
Bone regeneration
Critical bone defect
Hydroxyapatite
Strontium.
Biomateriales
Regeneración ósea
Defecto óseo crítico
Hidroxiapatita
Estroncio.
Biomateriais
Regeneração Óssea
Defeito ósseo crítico
Hidroxiapatita
Estrôncio.
topic Biomaterials
Bone regeneration
Critical bone defect
Hydroxyapatite
Strontium.
Biomateriales
Regeneración ósea
Defecto óseo crítico
Hidroxiapatita
Estroncio.
Biomateriais
Regeneração Óssea
Defeito ósseo crítico
Hidroxiapatita
Estrôncio.
description The aim of this study was to analyze the biological behavior and osteogenic potential of nanostructured hydroxyapatite microspheres substituted with strontium (nHASr). Therefore, twenty adult male Wistar rats were randomly distributed into two groups: GnHASr – critical bone defect filled with nHASr microspheres; e CG (control group) – critical bone defect without implantation of biomaterial; evaluated at the biological points of 30 and 60 days. The collected specimens were processed and stained with hematoxylin-eosin (HE) and Masson-Goldner trichrome (TG) and examined by light microscopy. Posteriorly, they were analyzed histomorphometrically to measure the percentage of neoformed osteoid matrix (%OM). In both groups studied, at all biological points, deposition of reparative osteoid matrix (OM) was observed near the bone edges; discrete chronic inflammatory response; connective tissue formation and neovascularization in the residual area of the defect. In the GnHASr, in the two evaluated periods, the deposition of OM was also noticed, both around and inside the microspheres. At 60 days, an area of 7.54% of OM deposition in relation to the total defect area was evidenced in the GnHASr, while in the CG this value was 6.80%. It is concluded that the nHASr microspheres evaluated were biocompatible, biodegradable, bioresorbable, bioactive and osteoconductive. In both groups, the formation of neomineralized tissue occurred in a limited way, which indicates that the concentration of metal used in the replacement did not favor greater osteogenic potential for the biomaterial. The evaluated biomaterial it's adequate for use as a filling material.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/41222
10.33448/rsd-v12i4.41222
url https://rsdjournal.org/index.php/rsd/article/view/41222
identifier_str_mv 10.33448/rsd-v12i4.41222
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/41222/33546
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 12 No. 4; e22112441222
Research, Society and Development; Vol. 12 Núm. 4; e22112441222
Research, Society and Development; v. 12 n. 4; e22112441222
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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