Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/41222 |
Resumo: | The aim of this study was to analyze the biological behavior and osteogenic potential of nanostructured hydroxyapatite microspheres substituted with strontium (nHASr). Therefore, twenty adult male Wistar rats were randomly distributed into two groups: GnHASr – critical bone defect filled with nHASr microspheres; e CG (control group) – critical bone defect without implantation of biomaterial; evaluated at the biological points of 30 and 60 days. The collected specimens were processed and stained with hematoxylin-eosin (HE) and Masson-Goldner trichrome (TG) and examined by light microscopy. Posteriorly, they were analyzed histomorphometrically to measure the percentage of neoformed osteoid matrix (%OM). In both groups studied, at all biological points, deposition of reparative osteoid matrix (OM) was observed near the bone edges; discrete chronic inflammatory response; connective tissue formation and neovascularization in the residual area of the defect. In the GnHASr, in the two evaluated periods, the deposition of OM was also noticed, both around and inside the microspheres. At 60 days, an area of 7.54% of OM deposition in relation to the total defect area was evidenced in the GnHASr, while in the CG this value was 6.80%. It is concluded that the nHASr microspheres evaluated were biocompatible, biodegradable, bioresorbable, bioactive and osteoconductive. In both groups, the formation of neomineralized tissue occurred in a limited way, which indicates that the concentration of metal used in the replacement did not favor greater osteogenic potential for the biomaterial. The evaluated biomaterial it's adequate for use as a filling material. |
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Strontium-containing nanostructured hydroxyapatite microspheres for bone regenerationMicroesferas de hidroxiapatita nanoestructuradas sustituidas con estroncio para la regeneración óseaMicroesferas de hidroxiapatita nanoestruturada substituída por estrôncio para regeneração ósseaBiomaterialsBone regenerationCritical bone defectHydroxyapatiteStrontium.BiomaterialesRegeneración óseaDefecto óseo críticoHidroxiapatitaEstroncio.BiomateriaisRegeneração ÓsseaDefeito ósseo críticoHidroxiapatitaEstrôncio.The aim of this study was to analyze the biological behavior and osteogenic potential of nanostructured hydroxyapatite microspheres substituted with strontium (nHASr). Therefore, twenty adult male Wistar rats were randomly distributed into two groups: GnHASr – critical bone defect filled with nHASr microspheres; e CG (control group) – critical bone defect without implantation of biomaterial; evaluated at the biological points of 30 and 60 days. The collected specimens were processed and stained with hematoxylin-eosin (HE) and Masson-Goldner trichrome (TG) and examined by light microscopy. Posteriorly, they were analyzed histomorphometrically to measure the percentage of neoformed osteoid matrix (%OM). In both groups studied, at all biological points, deposition of reparative osteoid matrix (OM) was observed near the bone edges; discrete chronic inflammatory response; connective tissue formation and neovascularization in the residual area of the defect. In the GnHASr, in the two evaluated periods, the deposition of OM was also noticed, both around and inside the microspheres. At 60 days, an area of 7.54% of OM deposition in relation to the total defect area was evidenced in the GnHASr, while in the CG this value was 6.80%. It is concluded that the nHASr microspheres evaluated were biocompatible, biodegradable, bioresorbable, bioactive and osteoconductive. In both groups, the formation of neomineralized tissue occurred in a limited way, which indicates that the concentration of metal used in the replacement did not favor greater osteogenic potential for the biomaterial. The evaluated biomaterial it's adequate for use as a filling material.El objetivo de este estudio fue analizar el comportamiento biológico y el potencial osteogénico de microesferas de hidroxiapatita nanoestructuradas sustituidas con estrôncio (nHASr). Para tanto, veinte ratas wistar macho adultas se distribuyeron aleatoriamente en dos grupos: GnHASr – defecto óseo crítico relleno con microesferas nHASr; e GC (grupo de control) - defecto óseo crítico sin implantación de biomaterial; evaluado en los puntos biológicos de 30 y 60 días. Los especímenes recolectados fueron processados y teñidos con hematoxilina-eosina (HE) y tricrómico de Masson-Goldner (TG), y examinados por microscopía de luz común. Posteriormente, se analizaron histomorfométricamente para medir el porcentaje de matriz osteoide neoformada (%MO). En ambos grupos estudiados, en todos los puntos biológicos se observó depósito de matriz osteoide (MO) reparadora, cerca de los bordes óseos; respuesta inflamatoria crónica leve; formación de tejido conectivo y neovascularización en el área residual del defecto. En el GnHASr, en los dos periodos evaluados, también se notó la deposición de MO, tanto alrededor como dentro de las microesferas. A los 60 días se evidenció en el GnHASr un área de 7,54% de depósito de MO con relación al área total del defecto, mientras que en el GC este valor fue de 6,80%. Se concluye que las microesferas de nHASr evaluadas en este estudio fueron biocompatibles, biodegradables, biorreabsorbibles, bioactivas y osteoconductoras. En ambos grupos, la formación de tejido neomineralizado ocurrió de forma limitada, lo que indica que la concentración de metal utilizada en el reemplazo no favoreció un mayor potencial osteogénico para el biomaterial. El biomaterial evaluado es adecuado para su uso como material de relleno.O objetivo deste estudo foi analisar o comportamento biológico e potencial osteogênico de microesferas de hidroxiapatita nanoestruturadas substituídas com estrôncio (nHASr). Para tanto, utilizou-se vinte ratos wistar, adultos, machos, distribuídos, aleatoriamente, em dois grupos: GnHASr – defeito ósseo crítico preenchido com microesferas de nHASr; e GC (grupo controle) – defeito ósseo crítico sem implantação de biomaterial; avaliados nos pontos biológicos de 30 e 60 dias. Os espécimes foram processados e corados por hematoxilina-eosina (HE) e tricrômico de Masson-Goldner (TG), e examinados por microscopia de luz comum. Posteriormente, foram analisados histomorfometricamente, para mensuração do percentual de matriz osteoide neoformada (%MO). Nos dois grupos estudados, em todos os pontos biológicos, observou-se deposição de matriz osteoide (MO) reparativa, próxima às bordas ósseas; resposta inflamatória crônica discreta; formação de tecido conjuntivo e neovascularização na área residual do defeito. No GnHASr, nos dois períodos avaliados, a deposição de MO foi notada, também, tanto de forma circunjacente quanto no interior das microesferas. Aos 60 dias, evidenciou-se no GnHASr uma área de 7,54% de deposição de MO em relação a área total do defeito, enquanto no GC este valor foi de 6,80%. Conclui-se que as microesferas de nHASr avaliadas neste estudo foram biocompatíveis, biodegradáveis, biorreabsorvíveis, bioativas e osteocondutoras. Nos dois grupos, a formação de tecido neomineralizado ocorreu de forma limitada, isto indica que a concentração de metal utilizada na substituição não favoreceu maior potencial osteogênico ao biomaterial. O biomaterial avaliado é adequado para ser utilizado como material de preenchimento.Research, Society and Development2023-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4122210.33448/rsd-v12i4.41222Research, Society and Development; Vol. 12 No. 4; e22112441222Research, Society and Development; Vol. 12 Núm. 4; e22112441222Research, Society and Development; v. 12 n. 4; e221124412222525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/41222/33546Copyright (c) 2023 Iorrana Índira dos Anjos Ribeiro; Aryon de Almeida Barbosa Junior; Alexandre Malta Rossi; Renata dos Santos Almeida; Fúlvio Borges Miguel; Fabiana Paim Rosahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRibeiro, Iorrana Índira dos Anjos Barbosa Junior, Aryon de Almeida Rossi, Alexandre Malta Almeida, Renata dos SantosMiguel, Fúlvio BorgesRosa, Fabiana Paim2023-04-21T18:13:32Zoai:ojs.pkp.sfu.ca:article/41222Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-04-21T18:13:32Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration Microesferas de hidroxiapatita nanoestructuradas sustituidas con estroncio para la regeneración ósea Microesferas de hidroxiapatita nanoestruturada substituída por estrôncio para regeneração óssea |
title |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration |
spellingShingle |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration Ribeiro, Iorrana Índira dos Anjos Biomaterials Bone regeneration Critical bone defect Hydroxyapatite Strontium. Biomateriales Regeneración ósea Defecto óseo crítico Hidroxiapatita Estroncio. Biomateriais Regeneração Óssea Defeito ósseo crítico Hidroxiapatita Estrôncio. |
title_short |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration |
title_full |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration |
title_fullStr |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration |
title_full_unstemmed |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration |
title_sort |
Strontium-containing nanostructured hydroxyapatite microspheres for bone regeneration |
author |
Ribeiro, Iorrana Índira dos Anjos |
author_facet |
Ribeiro, Iorrana Índira dos Anjos Barbosa Junior, Aryon de Almeida Rossi, Alexandre Malta Almeida, Renata dos Santos Miguel, Fúlvio Borges Rosa, Fabiana Paim |
author_role |
author |
author2 |
Barbosa Junior, Aryon de Almeida Rossi, Alexandre Malta Almeida, Renata dos Santos Miguel, Fúlvio Borges Rosa, Fabiana Paim |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Iorrana Índira dos Anjos Barbosa Junior, Aryon de Almeida Rossi, Alexandre Malta Almeida, Renata dos Santos Miguel, Fúlvio Borges Rosa, Fabiana Paim |
dc.subject.por.fl_str_mv |
Biomaterials Bone regeneration Critical bone defect Hydroxyapatite Strontium. Biomateriales Regeneración ósea Defecto óseo crítico Hidroxiapatita Estroncio. Biomateriais Regeneração Óssea Defeito ósseo crítico Hidroxiapatita Estrôncio. |
topic |
Biomaterials Bone regeneration Critical bone defect Hydroxyapatite Strontium. Biomateriales Regeneración ósea Defecto óseo crítico Hidroxiapatita Estroncio. Biomateriais Regeneração Óssea Defeito ósseo crítico Hidroxiapatita Estrôncio. |
description |
The aim of this study was to analyze the biological behavior and osteogenic potential of nanostructured hydroxyapatite microspheres substituted with strontium (nHASr). Therefore, twenty adult male Wistar rats were randomly distributed into two groups: GnHASr – critical bone defect filled with nHASr microspheres; e CG (control group) – critical bone defect without implantation of biomaterial; evaluated at the biological points of 30 and 60 days. The collected specimens were processed and stained with hematoxylin-eosin (HE) and Masson-Goldner trichrome (TG) and examined by light microscopy. Posteriorly, they were analyzed histomorphometrically to measure the percentage of neoformed osteoid matrix (%OM). In both groups studied, at all biological points, deposition of reparative osteoid matrix (OM) was observed near the bone edges; discrete chronic inflammatory response; connective tissue formation and neovascularization in the residual area of the defect. In the GnHASr, in the two evaluated periods, the deposition of OM was also noticed, both around and inside the microspheres. At 60 days, an area of 7.54% of OM deposition in relation to the total defect area was evidenced in the GnHASr, while in the CG this value was 6.80%. It is concluded that the nHASr microspheres evaluated were biocompatible, biodegradable, bioresorbable, bioactive and osteoconductive. In both groups, the formation of neomineralized tissue occurred in a limited way, which indicates that the concentration of metal used in the replacement did not favor greater osteogenic potential for the biomaterial. The evaluated biomaterial it's adequate for use as a filling material. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/41222 10.33448/rsd-v12i4.41222 |
url |
https://rsdjournal.org/index.php/rsd/article/view/41222 |
identifier_str_mv |
10.33448/rsd-v12i4.41222 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/41222/33546 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 12 No. 4; e22112441222 Research, Society and Development; Vol. 12 Núm. 4; e22112441222 Research, Society and Development; v. 12 n. 4; e22112441222 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052622703689728 |