Cell block technique as an additional tool in the diagnosis of ameloblastoma

Detalhes bibliográficos
Autor(a) principal: BELATTO,Maria Fernanda da Silva
Data de Publicação: 2014
Outros Autores: RAMOS,Grasieli de Oliveira, MARTINS,Manoela Domingues, GRANDO,Liliane Janete, RIVERO,Elena Riet Correa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100253
Resumo: The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.
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spelling Cell block technique as an additional tool in the diagnosis of ameloblastomaBiopsyFine-NeedleCell BiologyOdontogenic TumorsAmeloblastomaThe objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100253Brazilian Oral Research v.28 n.1 2014reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2014.vol28.0044info:eu-repo/semantics/openAccessBELATTO,Maria Fernanda da SilvaRAMOS,Grasieli de OliveiraMARTINS,Manoela DominguesGRANDO,Liliane JaneteRIVERO,Elena Riet Correaeng2018-09-17T00:00:00Zoai:scielo:S1806-83242014000100253Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-09-17T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Cell block technique as an additional tool in the diagnosis of ameloblastoma
title Cell block technique as an additional tool in the diagnosis of ameloblastoma
spellingShingle Cell block technique as an additional tool in the diagnosis of ameloblastoma
BELATTO,Maria Fernanda da Silva
Biopsy
Fine-Needle
Cell Biology
Odontogenic Tumors
Ameloblastoma
title_short Cell block technique as an additional tool in the diagnosis of ameloblastoma
title_full Cell block technique as an additional tool in the diagnosis of ameloblastoma
title_fullStr Cell block technique as an additional tool in the diagnosis of ameloblastoma
title_full_unstemmed Cell block technique as an additional tool in the diagnosis of ameloblastoma
title_sort Cell block technique as an additional tool in the diagnosis of ameloblastoma
author BELATTO,Maria Fernanda da Silva
author_facet BELATTO,Maria Fernanda da Silva
RAMOS,Grasieli de Oliveira
MARTINS,Manoela Domingues
GRANDO,Liliane Janete
RIVERO,Elena Riet Correa
author_role author
author2 RAMOS,Grasieli de Oliveira
MARTINS,Manoela Domingues
GRANDO,Liliane Janete
RIVERO,Elena Riet Correa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv BELATTO,Maria Fernanda da Silva
RAMOS,Grasieli de Oliveira
MARTINS,Manoela Domingues
GRANDO,Liliane Janete
RIVERO,Elena Riet Correa
dc.subject.por.fl_str_mv Biopsy
Fine-Needle
Cell Biology
Odontogenic Tumors
Ameloblastoma
topic Biopsy
Fine-Needle
Cell Biology
Odontogenic Tumors
Ameloblastoma
description The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100253
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107BOR-2014.vol28.0044
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.28 n.1 2014
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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