Cell block technique as an additional tool in the diagnosis of ameloblastoma

• Autor(a) principal: Belatto, Maria Fernanda da Silva
• Data de Publicação: 2014
• Outros Autores: Ramos, Grasieli de Oliveira, Martins, Manoela Domingues, Grando, Liliane Janete, Rivero, Helena Riet Correa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/106722
• topic: Ameloblastoma; Tumores : Odontogenicos; Biópsia por agulha fina; Biopsy; Fine-needle; Cell biology; Odontogenic tumors; Ameloblastoma

The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/ AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.