Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000200007 |
Resumo: | Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis. |
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Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimationCyclosporineStereological analysisBiochemical analysisGingival hyperplasiaCyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000200007Brazilian Oral Research v.19 n.2 2005reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242005000200007info:eu-repo/semantics/openAccessSpolidorio,Luis CarlosSpolidorio,Denise Madalena PalomariHolzhausen,MarinellaNassar,Patricia OehlmeyerNassar,Carlos Augustoeng2005-09-08T00:00:00Zoai:scielo:S1806-83242005000200007Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2005-09-08T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
title |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
spellingShingle |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation Spolidorio,Luis Carlos Cyclosporine Stereological analysis Biochemical analysis Gingival hyperplasia |
title_short |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
title_full |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
title_fullStr |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
title_full_unstemmed |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
title_sort |
Effects of long-term cyclosporin therapy on gingiva of rats: analysis by stereological and biochemical estimation |
author |
Spolidorio,Luis Carlos |
author_facet |
Spolidorio,Luis Carlos Spolidorio,Denise Madalena Palomari Holzhausen,Marinella Nassar,Patricia Oehlmeyer Nassar,Carlos Augusto |
author_role |
author |
author2 |
Spolidorio,Denise Madalena Palomari Holzhausen,Marinella Nassar,Patricia Oehlmeyer Nassar,Carlos Augusto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Spolidorio,Luis Carlos Spolidorio,Denise Madalena Palomari Holzhausen,Marinella Nassar,Patricia Oehlmeyer Nassar,Carlos Augusto |
dc.subject.por.fl_str_mv |
Cyclosporine Stereological analysis Biochemical analysis Gingival hyperplasia |
topic |
Cyclosporine Stereological analysis Biochemical analysis Gingival hyperplasia |
description |
Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000200007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242005000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242005000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.19 n.2 2005 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318320379232256 |