Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242005000200007 http://hdl.handle.net/11449/68185 |
Resumo: | Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis. |
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Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.collagencyclosporinimmunosuppressive agenttransforming growth factor betaanimalcell countchemically induced disorderchemistrydrug effectenzyme linked immunosorbent assayfibroblastgingivagingiva hyperplasiamalemetabolismpathologyratsalivatimeWistar ratAnimalsCell CountCollagenCyclosporineEnzyme-Linked Immunosorbent AssayFibroblastsGingivaGingival HyperplasiaImmunosuppressive AgentsMaleRatsRats, WistarSalivaTime FactorsTransforming Growth Factor betaCyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis.Universidade Estadual Paulista (Unesp)Spolidório, Luis Carlos [UNESP]Spolidório, Denise Madalena Palomari [UNESP]Holzhausen, MarinellaNassar, Patricia OehlmeyerNassar, Carlos Augusto2014-05-27T11:21:18Z2014-05-27T11:21:18Z2005-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article112-118application/pdfhttp://dx.doi.org/10.1590/S1806-83242005000200007Pesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 2, p. 112-118, 2005.1806-8324http://hdl.handle.net/11449/6818510.1590/S1806-83242005000200007S1806-832420050002000072-s2.0-336458774132-s2.0-33645877413.pdf2640929291808415Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Odontológica Brasileira = Brazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-09-27T14:04:48Zoai:repositorio.unesp.br:11449/68185Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:04:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
title |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
spellingShingle |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. Spolidório, Luis Carlos [UNESP] collagen cyclosporin immunosuppressive agent transforming growth factor beta animal cell count chemically induced disorder chemistry drug effect enzyme linked immunosorbent assay fibroblast gingiva gingiva hyperplasia male metabolism pathology rat saliva time Wistar rat Animals Cell Count Collagen Cyclosporine Enzyme-Linked Immunosorbent Assay Fibroblasts Gingiva Gingival Hyperplasia Immunosuppressive Agents Male Rats Rats, Wistar Saliva Time Factors Transforming Growth Factor beta |
title_short |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
title_full |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
title_fullStr |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
title_full_unstemmed |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
title_sort |
Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation. |
author |
Spolidório, Luis Carlos [UNESP] |
author_facet |
Spolidório, Luis Carlos [UNESP] Spolidório, Denise Madalena Palomari [UNESP] Holzhausen, Marinella Nassar, Patricia Oehlmeyer Nassar, Carlos Augusto |
author_role |
author |
author2 |
Spolidório, Denise Madalena Palomari [UNESP] Holzhausen, Marinella Nassar, Patricia Oehlmeyer Nassar, Carlos Augusto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Spolidório, Luis Carlos [UNESP] Spolidório, Denise Madalena Palomari [UNESP] Holzhausen, Marinella Nassar, Patricia Oehlmeyer Nassar, Carlos Augusto |
dc.subject.por.fl_str_mv |
collagen cyclosporin immunosuppressive agent transforming growth factor beta animal cell count chemically induced disorder chemistry drug effect enzyme linked immunosorbent assay fibroblast gingiva gingiva hyperplasia male metabolism pathology rat saliva time Wistar rat Animals Cell Count Collagen Cyclosporine Enzyme-Linked Immunosorbent Assay Fibroblasts Gingiva Gingival Hyperplasia Immunosuppressive Agents Male Rats Rats, Wistar Saliva Time Factors Transforming Growth Factor beta |
topic |
collagen cyclosporin immunosuppressive agent transforming growth factor beta animal cell count chemically induced disorder chemistry drug effect enzyme linked immunosorbent assay fibroblast gingiva gingiva hyperplasia male metabolism pathology rat saliva time Wistar rat Animals Cell Count Collagen Cyclosporine Enzyme-Linked Immunosorbent Assay Fibroblasts Gingiva Gingival Hyperplasia Immunosuppressive Agents Male Rats Rats, Wistar Saliva Time Factors Transforming Growth Factor beta |
description |
Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-04-01 2014-05-27T11:21:18Z 2014-05-27T11:21:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242005000200007 Pesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 2, p. 112-118, 2005. 1806-8324 http://hdl.handle.net/11449/68185 10.1590/S1806-83242005000200007 S1806-83242005000200007 2-s2.0-33645877413 2-s2.0-33645877413.pdf 2640929291808415 |
url |
http://dx.doi.org/10.1590/S1806-83242005000200007 http://hdl.handle.net/11449/68185 |
identifier_str_mv |
Pesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 2, p. 112-118, 2005. 1806-8324 10.1590/S1806-83242005000200007 S1806-83242005000200007 2-s2.0-33645877413 2-s2.0-33645877413.pdf 2640929291808415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Odontológica Brasileira = Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
112-118 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546407613693952 |