Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.

Detalhes bibliográficos
Autor(a) principal: Spolidório, Luis Carlos [UNESP]
Data de Publicação: 2005
Outros Autores: Spolidório, Denise Madalena Palomari [UNESP], Holzhausen, Marinella, Nassar, Patricia Oehlmeyer, Nassar, Carlos Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1806-83242005000200007
http://hdl.handle.net/11449/68185
Resumo: Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis.
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spelling Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.collagencyclosporinimmunosuppressive agenttransforming growth factor betaanimalcell countchemically induced disorderchemistrydrug effectenzyme linked immunosorbent assayfibroblastgingivagingiva hyperplasiamalemetabolismpathologyratsalivatimeWistar ratAnimalsCell CountCollagenCyclosporineEnzyme-Linked Immunosorbent AssayFibroblastsGingivaGingival HyperplasiaImmunosuppressive AgentsMaleRatsRats, WistarSalivaTime FactorsTransforming Growth Factor betaCyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis.Universidade Estadual Paulista (Unesp)Spolidório, Luis Carlos [UNESP]Spolidório, Denise Madalena Palomari [UNESP]Holzhausen, MarinellaNassar, Patricia OehlmeyerNassar, Carlos Augusto2014-05-27T11:21:18Z2014-05-27T11:21:18Z2005-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article112-118application/pdfhttp://dx.doi.org/10.1590/S1806-83242005000200007Pesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 2, p. 112-118, 2005.1806-8324http://hdl.handle.net/11449/6818510.1590/S1806-83242005000200007S1806-832420050002000072-s2.0-336458774132-s2.0-33645877413.pdf2640929291808415Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Odontológica Brasileira = Brazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-09-27T14:04:48Zoai:repositorio.unesp.br:11449/68185Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:04:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
title Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
spellingShingle Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
Spolidório, Luis Carlos [UNESP]
collagen
cyclosporin
immunosuppressive agent
transforming growth factor beta
animal
cell count
chemically induced disorder
chemistry
drug effect
enzyme linked immunosorbent assay
fibroblast
gingiva
gingiva hyperplasia
male
metabolism
pathology
rat
saliva
time
Wistar rat
Animals
Cell Count
Collagen
Cyclosporine
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Gingiva
Gingival Hyperplasia
Immunosuppressive Agents
Male
Rats
Rats, Wistar
Saliva
Time Factors
Transforming Growth Factor beta
title_short Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
title_full Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
title_fullStr Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
title_full_unstemmed Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
title_sort Effects of long-term cyclosporin therapy on gingiva of rats--analysis by stereological and biochemical estimation.
author Spolidório, Luis Carlos [UNESP]
author_facet Spolidório, Luis Carlos [UNESP]
Spolidório, Denise Madalena Palomari [UNESP]
Holzhausen, Marinella
Nassar, Patricia Oehlmeyer
Nassar, Carlos Augusto
author_role author
author2 Spolidório, Denise Madalena Palomari [UNESP]
Holzhausen, Marinella
Nassar, Patricia Oehlmeyer
Nassar, Carlos Augusto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Spolidório, Luis Carlos [UNESP]
Spolidório, Denise Madalena Palomari [UNESP]
Holzhausen, Marinella
Nassar, Patricia Oehlmeyer
Nassar, Carlos Augusto
dc.subject.por.fl_str_mv collagen
cyclosporin
immunosuppressive agent
transforming growth factor beta
animal
cell count
chemically induced disorder
chemistry
drug effect
enzyme linked immunosorbent assay
fibroblast
gingiva
gingiva hyperplasia
male
metabolism
pathology
rat
saliva
time
Wistar rat
Animals
Cell Count
Collagen
Cyclosporine
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Gingiva
Gingival Hyperplasia
Immunosuppressive Agents
Male
Rats
Rats, Wistar
Saliva
Time Factors
Transforming Growth Factor beta
topic collagen
cyclosporin
immunosuppressive agent
transforming growth factor beta
animal
cell count
chemically induced disorder
chemistry
drug effect
enzyme linked immunosorbent assay
fibroblast
gingiva
gingiva hyperplasia
male
metabolism
pathology
rat
saliva
time
Wistar rat
Animals
Cell Count
Collagen
Cyclosporine
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Gingiva
Gingival Hyperplasia
Immunosuppressive Agents
Male
Rats
Rats, Wistar
Saliva
Time Factors
Transforming Growth Factor beta
description Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth, which remains a significant problem. The risk factors appraised include the duration of treatment. However, there are no stereological and biochemical studies exploring the effects of long-term CsA therapy on gingival tissue. The purpose of the present study was to investigate the level of TGF-beta1 in saliva and describe the densities of fibroblasts and collagen fibers in the gingival tissue of rats treated with CsA for long periods. Rats were treated for 60, 120, 180 and 240 days with a daily subcutaneous injection of 10 mg/kg of body weight of CsA. At the end of the experimental periods, saliva was collected for the determination of TGF-beta1 levels. After histological processing, the oral epithelium and the connective tissue area were measured as well as the volume densities of fibroblasts (Vf) and collagen fibers (Vcf). After 60 and 120 days of CsA treatment, there was a significant increase in Vf and Vcf as well as a significant increase in TGF-beta1 levels. After 180 and 240 days, reduction in the gingival overgrowth associated with significant decreases in the level of TGF-beta1, and also decreased Vf and Vcf, were observed. The data presented here suggest that after long-term therapy, a decrease in TGF-beta1 levels occurs, which might contribute to an increase in the proteolytic activity of fibroblasts in the gingiva, favoring the normality of extracellular matrix synthesis.
publishDate 2005
dc.date.none.fl_str_mv 2005-04-01
2014-05-27T11:21:18Z
2014-05-27T11:21:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1806-83242005000200007
Pesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 2, p. 112-118, 2005.
1806-8324
http://hdl.handle.net/11449/68185
10.1590/S1806-83242005000200007
S1806-83242005000200007
2-s2.0-33645877413
2-s2.0-33645877413.pdf
2640929291808415
url http://dx.doi.org/10.1590/S1806-83242005000200007
http://hdl.handle.net/11449/68185
identifier_str_mv Pesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 2, p. 112-118, 2005.
1806-8324
10.1590/S1806-83242005000200007
S1806-83242005000200007
2-s2.0-33645877413
2-s2.0-33645877413.pdf
2640929291808415
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Odontológica Brasileira = Brazilian Oral Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 112-118
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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