Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Brazilian Oral Research |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242007000200006 |
Resumo: | A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh. |
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Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridizationOdontogenesisTooth germWnt proteinsBone morphogenetic proteinsA comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242007000200006Brazilian Oral Research v.21 n.2 2007reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242007000200006info:eu-repo/semantics/openAccessNunes,Fábio DaumasValenzuela,Maria da Graça SilvaRodini,Camila OliveiraMassironi,Silvia Maria GomesKo,Gui Mieng2009-10-19T00:00:00Zoai:scielo:S1806-83242007000200006Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2009-10-19T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
| dc.title.none.fl_str_mv |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| title |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| spellingShingle |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization Nunes,Fábio Daumas Odontogenesis Tooth germ Wnt proteins Bone morphogenetic proteins |
| title_short |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| title_full |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| title_fullStr |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| title_full_unstemmed |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| title_sort |
Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization |
| author |
Nunes,Fábio Daumas |
| author_facet |
Nunes,Fábio Daumas Valenzuela,Maria da Graça Silva Rodini,Camila Oliveira Massironi,Silvia Maria Gomes Ko,Gui Mi |
| author_role |
author |
| author2 |
Valenzuela,Maria da Graça Silva Rodini,Camila Oliveira Massironi,Silvia Maria Gomes Ko,Gui Mi |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Nunes,Fábio Daumas Valenzuela,Maria da Graça Silva Rodini,Camila Oliveira Massironi,Silvia Maria Gomes Ko,Gui Mi |
| dc.subject.por.fl_str_mv |
Odontogenesis Tooth germ Wnt proteins Bone morphogenetic proteins |
| topic |
Odontogenesis Tooth germ Wnt proteins Bone morphogenetic proteins |
| description |
A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-06-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242007000200006 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242007000200006 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S1806-83242007000200006 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
| dc.source.none.fl_str_mv |
Brazilian Oral Research v.21 n.2 2007 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
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Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
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SBPQO |
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SBPQO |
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Brazilian Oral Research |
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Brazilian Oral Research |
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Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
| repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318320844800000 |