Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization

Detalhes bibliográficos
Autor(a) principal: Nunes, Fábio Daumas
Data de Publicação: 2007
Outros Autores: Valenzuela, Maria Da Graça Silva, Rodini, Camila Oliveira, Massironi, Silvia Maria Gomes, Ko, Gui Mi [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000h1j7
Texto Completo: http://dx.doi.org/10.1590/S1806-83242007000200006
http://repositorio.unifesp.br/handle/11600/3749
Resumo: A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh.
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spelling Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridizationLocalização de transcritos de Bmp-4, Shh e Wnt-5a durante as fases iniciais do desenvolvimento dentário de camundongos por hibridização in situOdontogenesisTooth germWnt proteinsBone morphogenetic proteinsOdontogêneseGerme de denteProteínas WntProteínas morfogenéticas ósseasA comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh.No presente trabalho, realizou-se uma análise comparativa não isotópica por hibridização in situ a fim de se detectar a presença de transcritos de Bmp-4, Shh e Wnt-5a durante as fases iniciais da odontogênese em camundongos, desde a iniciação até o estágio de capuz. No estágio de iniciação, observou-se expressão precoce de Bmp-4 no epitélio e no mesênquima subjacente, enquanto que a expressão de Shh ocorreu durante o estágio de capuz, na região de espessamento do revestimento epitelial odontogênico, tornando-se mais intensa na área de nó do esmalte. Os transcritos de Wnt-5a foram expressos somente no mesênquima durante os estágios de iniciação, botão e capuz, com intenso sinal na região no mesênquima na fase de botão. Estes resultados mostraram que Bmp-4, Shh e Wnt-5a são expressos desde os estágios mais precoces do desenvolvimento dentário, sugerindo que o gene Wnt-5a seja expresso em populações celulares distintas daquelas que expressam Bmp-4 e Shh.University of São Paulo School of Dentistry Department of Oral PathologyUniversity of São Paulo Biomedical Sciences Institute Department of ImmunologyFederal University of São Paulo Center for Development of Animal ModelsUNIFESP, Center for Development of Animal ModelsSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sociedade Brasileira de Pesquisa Odontológica - SBPqOUniversity of São Paulo School of Dentistry Department of Oral PathologyUniversity of São Paulo Biomedical Sciences Institute Department of ImmunologyUniversidade Federal de São Paulo (UNIFESP)Nunes, Fábio DaumasValenzuela, Maria Da Graça SilvaRodini, Camila OliveiraMassironi, Silvia Maria GomesKo, Gui Mi [UNIFESP]2015-06-14T13:36:57Z2015-06-14T13:36:57Z2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion127-133application/pdfhttp://dx.doi.org/10.1590/S1806-83242007000200006Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 21, n. 2, p. 127-133, 2007.10.1590/S1806-83242007000200006S1806-83242007000200006.pdf1806-8324S1806-83242007000200006http://repositorio.unifesp.br/handle/11600/3749ark:/48912/001300000h1j7engBrazilian Oral Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T12:55:48Zoai:repositorio.unifesp.br/:11600/3749Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:18:20.730426Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
Localização de transcritos de Bmp-4, Shh e Wnt-5a durante as fases iniciais do desenvolvimento dentário de camundongos por hibridização in situ
title Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
spellingShingle Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
Nunes, Fábio Daumas
Odontogenesis
Tooth germ
Wnt proteins
Bone morphogenetic proteins
Odontogênese
Germe de dente
Proteínas Wnt
Proteínas morfogenéticas ósseas
title_short Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
title_full Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
title_fullStr Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
title_full_unstemmed Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
title_sort Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization
author Nunes, Fábio Daumas
author_facet Nunes, Fábio Daumas
Valenzuela, Maria Da Graça Silva
Rodini, Camila Oliveira
Massironi, Silvia Maria Gomes
Ko, Gui Mi [UNIFESP]
author_role author
author2 Valenzuela, Maria Da Graça Silva
Rodini, Camila Oliveira
Massironi, Silvia Maria Gomes
Ko, Gui Mi [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv University of São Paulo School of Dentistry Department of Oral Pathology
University of São Paulo Biomedical Sciences Institute Department of Immunology
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Nunes, Fábio Daumas
Valenzuela, Maria Da Graça Silva
Rodini, Camila Oliveira
Massironi, Silvia Maria Gomes
Ko, Gui Mi [UNIFESP]
dc.subject.por.fl_str_mv Odontogenesis
Tooth germ
Wnt proteins
Bone morphogenetic proteins
Odontogênese
Germe de dente
Proteínas Wnt
Proteínas morfogenéticas ósseas
topic Odontogenesis
Tooth germ
Wnt proteins
Bone morphogenetic proteins
Odontogênese
Germe de dente
Proteínas Wnt
Proteínas morfogenéticas ósseas
description A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
2015-06-14T13:36:57Z
2015-06-14T13:36:57Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1806-83242007000200006
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 21, n. 2, p. 127-133, 2007.
10.1590/S1806-83242007000200006
S1806-83242007000200006.pdf
1806-8324
S1806-83242007000200006
http://repositorio.unifesp.br/handle/11600/3749
dc.identifier.dark.fl_str_mv ark:/48912/001300000h1j7
url http://dx.doi.org/10.1590/S1806-83242007000200006
http://repositorio.unifesp.br/handle/11600/3749
identifier_str_mv Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 21, n. 2, p. 127-133, 2007.
10.1590/S1806-83242007000200006
S1806-83242007000200006.pdf
1806-8324
S1806-83242007000200006
ark:/48912/001300000h1j7
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Oral Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 127-133
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1818602464412172288

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