Culture of human dental pulp cells at variable times post-tooth extraction

Detalhes bibliográficos
Autor(a) principal: Daniela Ferreira Araújo,BENÍCIO
Data de Publicação: 2018
Outros Autores: Luciana Oliveira,PEREIRA, Izabel Cristina Rodrigues da,SILVA, Ricardo Bentes,AZEVEDO, Ana Cristina Barreto,BEZERRA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202
Resumo: Abstract The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.
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spelling Culture of human dental pulp cells at variable times post-tooth extractionAdult Stem CellsDental PulpMesenchymal Stem CellStem Cell ResearchAbstract The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202Brazilian Oral Research v.32 2018reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2018.vol32.0003info:eu-repo/semantics/openAccessDaniela Ferreira Araújo,BENÍCIOLuciana Oliveira,PEREIRAIzabel Cristina Rodrigues da,SILVARicardo Bentes,AZEVEDOAna Cristina Barreto,BEZERRAeng2018-01-23T00:00:00Zoai:scielo:S1806-83242018000100202Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2018-01-23T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Culture of human dental pulp cells at variable times post-tooth extraction
title Culture of human dental pulp cells at variable times post-tooth extraction
spellingShingle Culture of human dental pulp cells at variable times post-tooth extraction
Daniela Ferreira Araújo,BENÍCIO
Adult Stem Cells
Dental Pulp
Mesenchymal Stem Cell
Stem Cell Research
title_short Culture of human dental pulp cells at variable times post-tooth extraction
title_full Culture of human dental pulp cells at variable times post-tooth extraction
title_fullStr Culture of human dental pulp cells at variable times post-tooth extraction
title_full_unstemmed Culture of human dental pulp cells at variable times post-tooth extraction
title_sort Culture of human dental pulp cells at variable times post-tooth extraction
author Daniela Ferreira Araújo,BENÍCIO
author_facet Daniela Ferreira Araújo,BENÍCIO
Luciana Oliveira,PEREIRA
Izabel Cristina Rodrigues da,SILVA
Ricardo Bentes,AZEVEDO
Ana Cristina Barreto,BEZERRA
author_role author
author2 Luciana Oliveira,PEREIRA
Izabel Cristina Rodrigues da,SILVA
Ricardo Bentes,AZEVEDO
Ana Cristina Barreto,BEZERRA
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Daniela Ferreira Araújo,BENÍCIO
Luciana Oliveira,PEREIRA
Izabel Cristina Rodrigues da,SILVA
Ricardo Bentes,AZEVEDO
Ana Cristina Barreto,BEZERRA
dc.subject.por.fl_str_mv Adult Stem Cells
Dental Pulp
Mesenchymal Stem Cell
Stem Cell Research
topic Adult Stem Cells
Dental Pulp
Mesenchymal Stem Cell
Stem Cell Research
description Abstract The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2018.vol32.0003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.32 2018
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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