Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335 |
Resumo: | ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair. |
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Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineeringmesenchymal stem cellsdental pulpmurinephenotypic characterizationcellular differentiationABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair.Instituto de Tecnologia do Paraná - Tecpar2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335Brazilian Archives of Biology and Technology v.59 2016reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2016150613info:eu-repo/semantics/openAccessBertassoli,Bruno MachadoCosta,Emanuela SilvaSousa,Cristiane AparecidaAlbergaria,Juliano Douglas SilvaMaltos,Kátia L. MeloGoes,Alfredo MirandaMatins,Thais Maria da MataSilva,Gerluza Aparecida BorgesJorge,Erika Cristinaeng2017-01-20T00:00:00Zoai:scielo:S1516-89132016000100335Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2017-01-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
title |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
spellingShingle |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering Bertassoli,Bruno Machado mesenchymal stem cells dental pulp murine phenotypic characterization cellular differentiation |
title_short |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
title_full |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
title_fullStr |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
title_full_unstemmed |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
title_sort |
Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering |
author |
Bertassoli,Bruno Machado |
author_facet |
Bertassoli,Bruno Machado Costa,Emanuela Silva Sousa,Cristiane Aparecida Albergaria,Juliano Douglas Silva Maltos,Kátia L. Melo Goes,Alfredo Miranda Matins,Thais Maria da Mata Silva,Gerluza Aparecida Borges Jorge,Erika Cristina |
author_role |
author |
author2 |
Costa,Emanuela Silva Sousa,Cristiane Aparecida Albergaria,Juliano Douglas Silva Maltos,Kátia L. Melo Goes,Alfredo Miranda Matins,Thais Maria da Mata Silva,Gerluza Aparecida Borges Jorge,Erika Cristina |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bertassoli,Bruno Machado Costa,Emanuela Silva Sousa,Cristiane Aparecida Albergaria,Juliano Douglas Silva Maltos,Kátia L. Melo Goes,Alfredo Miranda Matins,Thais Maria da Mata Silva,Gerluza Aparecida Borges Jorge,Erika Cristina |
dc.subject.por.fl_str_mv |
mesenchymal stem cells dental pulp murine phenotypic characterization cellular differentiation |
topic |
mesenchymal stem cells dental pulp murine phenotypic characterization cellular differentiation |
description |
ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2016150613 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.59 2016 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318277353013248 |