Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering

Detalhes bibliográficos
Autor(a) principal: Bertassoli,Bruno Machado
Data de Publicação: 2016
Outros Autores: Costa,Emanuela Silva, Sousa,Cristiane Aparecida, Albergaria,Juliano Douglas Silva, Maltos,Kátia L. Melo, Goes,Alfredo Miranda, Matins,Thais Maria da Mata, Silva,Gerluza Aparecida Borges, Jorge,Erika Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335
Resumo: ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair.
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spelling Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineeringmesenchymal stem cellsdental pulpmurinephenotypic characterizationcellular differentiationABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair.Instituto de Tecnologia do Paraná - Tecpar2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335Brazilian Archives of Biology and Technology v.59 2016reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2016150613info:eu-repo/semantics/openAccessBertassoli,Bruno MachadoCosta,Emanuela SilvaSousa,Cristiane AparecidaAlbergaria,Juliano Douglas SilvaMaltos,Kátia L. MeloGoes,Alfredo MirandaMatins,Thais Maria da MataSilva,Gerluza Aparecida BorgesJorge,Erika Cristinaeng2017-01-20T00:00:00Zoai:scielo:S1516-89132016000100335Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2017-01-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
title Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
spellingShingle Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
Bertassoli,Bruno Machado
mesenchymal stem cells
dental pulp
murine
phenotypic characterization
cellular differentiation
title_short Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
title_full Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
title_fullStr Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
title_full_unstemmed Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
title_sort Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
author Bertassoli,Bruno Machado
author_facet Bertassoli,Bruno Machado
Costa,Emanuela Silva
Sousa,Cristiane Aparecida
Albergaria,Juliano Douglas Silva
Maltos,Kátia L. Melo
Goes,Alfredo Miranda
Matins,Thais Maria da Mata
Silva,Gerluza Aparecida Borges
Jorge,Erika Cristina
author_role author
author2 Costa,Emanuela Silva
Sousa,Cristiane Aparecida
Albergaria,Juliano Douglas Silva
Maltos,Kátia L. Melo
Goes,Alfredo Miranda
Matins,Thais Maria da Mata
Silva,Gerluza Aparecida Borges
Jorge,Erika Cristina
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bertassoli,Bruno Machado
Costa,Emanuela Silva
Sousa,Cristiane Aparecida
Albergaria,Juliano Douglas Silva
Maltos,Kátia L. Melo
Goes,Alfredo Miranda
Matins,Thais Maria da Mata
Silva,Gerluza Aparecida Borges
Jorge,Erika Cristina
dc.subject.por.fl_str_mv mesenchymal stem cells
dental pulp
murine
phenotypic characterization
cellular differentiation
topic mesenchymal stem cells
dental pulp
murine
phenotypic characterization
cellular differentiation
description ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2016150613
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.59 2016
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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