A new experimental model of cigarette smoke-induced emphysema in Wistar rats

• Autor(a) principal: Kozma,Rodrigo de las Heras
• Data de Publicação: 2014
• Outros Autores: Alves,Edson Marcelino, Barbosa-de-Oliveira,Valter Abraao, Lopes,Fernanda Degobbi Tenorio Quirino dos Santos, Guardia,Renan Cenize, Buzo,Henrique Vivi, Faria,Carolina Arruda de, Yamashita,Camila, Cavazzana Junior,Manzelio, Frei,Fernando, Ribeiro-Paes,Maria Jose de Oliveira, Ribeiro-Paes,Joao Tadeu
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Jornal Brasileiro de Pneumologia (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132014000100046
• topic: Tobacco smoke pollution; Emphysema; Disease models, animal; Equipment and supplies

OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.