Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Pneumologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000200173 |
Resumo: | OBJECTIVE: To evaluate the effects that early and late systemic administration of methylprednisolone have on lungs in a rat model of brain death. METHODS: Twenty-four male Wistar rats were anesthetized and randomly divided into four groups (n = 6 per group): sham-operated (sham); brain death only (BD); brain death plus methylprednisolone (30 mg/kg i.v.) after 5 min (MP5); and brain death plus methylprednisolone (30 mg/kg i.v.) after 60 min (MP60). In the BD, MP5, and MP60 group rats, we induced brain death by inflating a balloon catheter in the extradural space. All of the animals were observed and ventilated for 120 min. We determined hemodynamic and arterial blood gas variables; wet/dry weight ratio; histological score; levels of thiobarbituric acid reactive substances (TBARS); superoxide dismutase (SOD) activity; and catalase activity. In BAL fluid, we determined differential white cell counts, total protein, and lactate dehydrogenase levels. Myeloperoxidase activity, lipid peroxidation, and TNF-α levels were assessed in lung tissue. RESULTS: No significant differences were found among the groups in terms of hemodynamics, arterial blood gases, wet/dry weight ratio, BAL fluid analysis, or histological score-nor in terms of SOD, myeloperoxidase, and catalase activity. The levels of TBARS were significantly higher in the MP5 and MP60 groups than in the sham and BD groups (p < 0.001). The levels of TNF-α were significantly lower in the MP5 and MP60 groups than in the BD group (p < 0.001). CONCLUSIONS: In this model of brain death, the early and late administration of methylprednisolone had similar effects on inflammatory activity and lipid peroxidation in lung tissue. |
id |
SBPT-1_c9bfa460427a74f8a63a04a1f53ecca8 |
---|---|
oai_identifier_str |
oai:scielo:S1806-37132013000200173 |
network_acronym_str |
SBPT-1 |
network_name_str |
Jornal Brasileiro de Pneumologia (Online) |
repository_id_str |
|
spelling |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead ratsRatsBrain deathOxidative stressLungHydroxycorticosteroids OBJECTIVE: To evaluate the effects that early and late systemic administration of methylprednisolone have on lungs in a rat model of brain death. METHODS: Twenty-four male Wistar rats were anesthetized and randomly divided into four groups (n = 6 per group): sham-operated (sham); brain death only (BD); brain death plus methylprednisolone (30 mg/kg i.v.) after 5 min (MP5); and brain death plus methylprednisolone (30 mg/kg i.v.) after 60 min (MP60). In the BD, MP5, and MP60 group rats, we induced brain death by inflating a balloon catheter in the extradural space. All of the animals were observed and ventilated for 120 min. We determined hemodynamic and arterial blood gas variables; wet/dry weight ratio; histological score; levels of thiobarbituric acid reactive substances (TBARS); superoxide dismutase (SOD) activity; and catalase activity. In BAL fluid, we determined differential white cell counts, total protein, and lactate dehydrogenase levels. Myeloperoxidase activity, lipid peroxidation, and TNF-α levels were assessed in lung tissue. RESULTS: No significant differences were found among the groups in terms of hemodynamics, arterial blood gases, wet/dry weight ratio, BAL fluid analysis, or histological score-nor in terms of SOD, myeloperoxidase, and catalase activity. The levels of TBARS were significantly higher in the MP5 and MP60 groups than in the sham and BD groups (p < 0.001). The levels of TNF-α were significantly lower in the MP5 and MP60 groups than in the BD group (p < 0.001). CONCLUSIONS: In this model of brain death, the early and late administration of methylprednisolone had similar effects on inflammatory activity and lipid peroxidation in lung tissue.Sociedade Brasileira de Pneumologia e Tisiologia2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000200173Jornal Brasileiro de Pneumologia v.39 n.2 2013reponame:Jornal Brasileiro de Pneumologia (Online)instname:Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)instacron:SBPT10.1590/S1806-37132013000200008info:eu-repo/semantics/openAccessPilla,Eduardo SperbPereira,Raôni BinsJunior,Luiz Alberto ForgiariniForgiarini,Luiz FelipePaludo,Artur de OliveiraKulczynski,Jane Maria UlbrichCardoso,Paulo Francisco GuerreiroAndrade,Cristiano Feijóeng2016-08-18T00:00:00Zoai:scielo:S1806-37132013000200173Revistahttp://www.jornaldepneumologia.com.br/default.aspONGhttps://old.scielo.br/oai/scielo-oai.php||jbp@jbp.org.br|| jpneumo@jornaldepneumologia.com.br1806-37561806-3713opendoar:2016-08-18T00:00Jornal Brasileiro de Pneumologia (Online) - Sociedade Brasileira de Pneumologia e Tisiologia (SBPT)false |
dc.title.none.fl_str_mv |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
title |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
spellingShingle |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats Pilla,Eduardo Sperb Rats Brain death Oxidative stress Lung Hydroxycorticosteroids |
title_short |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
title_full |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
title_fullStr |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
title_full_unstemmed |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
title_sort |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats |
author |
Pilla,Eduardo Sperb |
author_facet |
Pilla,Eduardo Sperb Pereira,Raôni Bins Junior,Luiz Alberto Forgiarini Forgiarini,Luiz Felipe Paludo,Artur de Oliveira Kulczynski,Jane Maria Ulbrich Cardoso,Paulo Francisco Guerreiro Andrade,Cristiano Feijó |
author_role |
author |
author2 |
Pereira,Raôni Bins Junior,Luiz Alberto Forgiarini Forgiarini,Luiz Felipe Paludo,Artur de Oliveira Kulczynski,Jane Maria Ulbrich Cardoso,Paulo Francisco Guerreiro Andrade,Cristiano Feijó |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pilla,Eduardo Sperb Pereira,Raôni Bins Junior,Luiz Alberto Forgiarini Forgiarini,Luiz Felipe Paludo,Artur de Oliveira Kulczynski,Jane Maria Ulbrich Cardoso,Paulo Francisco Guerreiro Andrade,Cristiano Feijó |
dc.subject.por.fl_str_mv |
Rats Brain death Oxidative stress Lung Hydroxycorticosteroids |
topic |
Rats Brain death Oxidative stress Lung Hydroxycorticosteroids |
description |
OBJECTIVE: To evaluate the effects that early and late systemic administration of methylprednisolone have on lungs in a rat model of brain death. METHODS: Twenty-four male Wistar rats were anesthetized and randomly divided into four groups (n = 6 per group): sham-operated (sham); brain death only (BD); brain death plus methylprednisolone (30 mg/kg i.v.) after 5 min (MP5); and brain death plus methylprednisolone (30 mg/kg i.v.) after 60 min (MP60). In the BD, MP5, and MP60 group rats, we induced brain death by inflating a balloon catheter in the extradural space. All of the animals were observed and ventilated for 120 min. We determined hemodynamic and arterial blood gas variables; wet/dry weight ratio; histological score; levels of thiobarbituric acid reactive substances (TBARS); superoxide dismutase (SOD) activity; and catalase activity. In BAL fluid, we determined differential white cell counts, total protein, and lactate dehydrogenase levels. Myeloperoxidase activity, lipid peroxidation, and TNF-α levels were assessed in lung tissue. RESULTS: No significant differences were found among the groups in terms of hemodynamics, arterial blood gases, wet/dry weight ratio, BAL fluid analysis, or histological score-nor in terms of SOD, myeloperoxidase, and catalase activity. The levels of TBARS were significantly higher in the MP5 and MP60 groups than in the sham and BD groups (p < 0.001). The levels of TNF-α were significantly lower in the MP5 and MP60 groups than in the BD group (p < 0.001). CONCLUSIONS: In this model of brain death, the early and late administration of methylprednisolone had similar effects on inflammatory activity and lipid peroxidation in lung tissue. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000200173 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000200173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-37132013000200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pneumologia e Tisiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pneumologia e Tisiologia |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Pneumologia v.39 n.2 2013 reponame:Jornal Brasileiro de Pneumologia (Online) instname:Sociedade Brasileira de Pneumologia e Tisiologia (SBPT) instacron:SBPT |
instname_str |
Sociedade Brasileira de Pneumologia e Tisiologia (SBPT) |
instacron_str |
SBPT |
institution |
SBPT |
reponame_str |
Jornal Brasileiro de Pneumologia (Online) |
collection |
Jornal Brasileiro de Pneumologia (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Pneumologia (Online) - Sociedade Brasileira de Pneumologia e Tisiologia (SBPT) |
repository.mail.fl_str_mv |
||jbp@jbp.org.br|| jpneumo@jornaldepneumologia.com.br |
_version_ |
1750318345486336000 |