Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006 |
Resumo: | In this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation. |
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Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applicationssuperparamagneticmagnetic nanoparticlescarboxyl-groupplasmid DNAIn this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation.Sociedade Brasileira de Química2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006Journal of the Brazilian Chemical Society v.18 n.7 2007reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532007000700006info:eu-repo/semantics/openAccessShan,ZhiYang,Wan-ShenZhang,XuHuang,Qian-MingYe,Huieng2008-01-08T00:00:00Zoai:scielo:S0103-50532007000700006Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2008-01-08T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
title |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
spellingShingle |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications Shan,Zhi superparamagnetic magnetic nanoparticles carboxyl-group plasmid DNA |
title_short |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
title_full |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
title_fullStr |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
title_full_unstemmed |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
title_sort |
Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications |
author |
Shan,Zhi |
author_facet |
Shan,Zhi Yang,Wan-Shen Zhang,Xu Huang,Qian-Ming Ye,Hui |
author_role |
author |
author2 |
Yang,Wan-Shen Zhang,Xu Huang,Qian-Ming Ye,Hui |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Shan,Zhi Yang,Wan-Shen Zhang,Xu Huang,Qian-Ming Ye,Hui |
dc.subject.por.fl_str_mv |
superparamagnetic magnetic nanoparticles carboxyl-group plasmid DNA |
topic |
superparamagnetic magnetic nanoparticles carboxyl-group plasmid DNA |
description |
In this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532007000700006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.18 n.7 2007 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318168254971904 |