Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications

Detalhes bibliográficos
Autor(a) principal: Shan,Zhi
Data de Publicação: 2007
Outros Autores: Yang,Wan-Shen, Zhang,Xu, Huang,Qian-Ming, Ye,Hui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006
Resumo: In this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation.
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spelling Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applicationssuperparamagneticmagnetic nanoparticlescarboxyl-groupplasmid DNAIn this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation.Sociedade Brasileira de Química2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006Journal of the Brazilian Chemical Society v.18 n.7 2007reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532007000700006info:eu-repo/semantics/openAccessShan,ZhiYang,Wan-ShenZhang,XuHuang,Qian-MingYe,Huieng2008-01-08T00:00:00Zoai:scielo:S0103-50532007000700006Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2008-01-08T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
title Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
spellingShingle Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
Shan,Zhi
superparamagnetic
magnetic nanoparticles
carboxyl-group
plasmid DNA
title_short Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
title_full Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
title_fullStr Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
title_full_unstemmed Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
title_sort Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications
author Shan,Zhi
author_facet Shan,Zhi
Yang,Wan-Shen
Zhang,Xu
Huang,Qian-Ming
Ye,Hui
author_role author
author2 Yang,Wan-Shen
Zhang,Xu
Huang,Qian-Ming
Ye,Hui
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Shan,Zhi
Yang,Wan-Shen
Zhang,Xu
Huang,Qian-Ming
Ye,Hui
dc.subject.por.fl_str_mv superparamagnetic
magnetic nanoparticles
carboxyl-group
plasmid DNA
topic superparamagnetic
magnetic nanoparticles
carboxyl-group
plasmid DNA
description In this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000700006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532007000700006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.18 n.7 2007
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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