A fluorescent-labeled microcystin-LR terbium cryptate

Detalhes bibliográficos
Autor(a) principal: Oliveira,Eduardo J. A.
Data de Publicação: 2006
Outros Autores: Vila Nova,Suzana P., Alves-Jr,Severino, Santa-Cruz,Petrus, Molica,Renato J. R., Teixeira,Antonio, Malageño,Elizabete, Lima Filho,José L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005
Resumo: A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well.
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spelling A fluorescent-labeled microcystin-LR terbium cryptateterbium cryptatemicrocystin-LRcyanobacteriaA new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well.Sociedade Brasileira de Química2006-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005Journal of the Brazilian Chemical Society v.17 n.2 2006reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532006000200005info:eu-repo/semantics/openAccessOliveira,Eduardo J. A.Vila Nova,Suzana P.Alves-Jr,SeverinoSanta-Cruz,PetrusMolica,Renato J. R.Teixeira,AntonioMalageño,ElizabeteLima Filho,José L.eng2006-04-19T00:00:00Zoai:scielo:S0103-50532006000200005Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2006-04-19T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A fluorescent-labeled microcystin-LR terbium cryptate
title A fluorescent-labeled microcystin-LR terbium cryptate
spellingShingle A fluorescent-labeled microcystin-LR terbium cryptate
Oliveira,Eduardo J. A.
terbium cryptate
microcystin-LR
cyanobacteria
title_short A fluorescent-labeled microcystin-LR terbium cryptate
title_full A fluorescent-labeled microcystin-LR terbium cryptate
title_fullStr A fluorescent-labeled microcystin-LR terbium cryptate
title_full_unstemmed A fluorescent-labeled microcystin-LR terbium cryptate
title_sort A fluorescent-labeled microcystin-LR terbium cryptate
author Oliveira,Eduardo J. A.
author_facet Oliveira,Eduardo J. A.
Vila Nova,Suzana P.
Alves-Jr,Severino
Santa-Cruz,Petrus
Molica,Renato J. R.
Teixeira,Antonio
Malageño,Elizabete
Lima Filho,José L.
author_role author
author2 Vila Nova,Suzana P.
Alves-Jr,Severino
Santa-Cruz,Petrus
Molica,Renato J. R.
Teixeira,Antonio
Malageño,Elizabete
Lima Filho,José L.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Eduardo J. A.
Vila Nova,Suzana P.
Alves-Jr,Severino
Santa-Cruz,Petrus
Molica,Renato J. R.
Teixeira,Antonio
Malageño,Elizabete
Lima Filho,José L.
dc.subject.por.fl_str_mv terbium cryptate
microcystin-LR
cyanobacteria
topic terbium cryptate
microcystin-LR
cyanobacteria
description A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well.
publishDate 2006
dc.date.none.fl_str_mv 2006-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532006000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.17 n.2 2006
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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