A fluorescent-labeled microcystin-LR terbium cryptate
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005 |
Resumo: | A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well. |
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Journal of the Brazilian Chemical Society (Online) |
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A fluorescent-labeled microcystin-LR terbium cryptateterbium cryptatemicrocystin-LRcyanobacteriaA new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well.Sociedade Brasileira de Química2006-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005Journal of the Brazilian Chemical Society v.17 n.2 2006reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532006000200005info:eu-repo/semantics/openAccessOliveira,Eduardo J. A.Vila Nova,Suzana P.Alves-Jr,SeverinoSanta-Cruz,PetrusMolica,Renato J. R.Teixeira,AntonioMalageño,ElizabeteLima Filho,José L.eng2006-04-19T00:00:00Zoai:scielo:S0103-50532006000200005Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2006-04-19T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A fluorescent-labeled microcystin-LR terbium cryptate |
title |
A fluorescent-labeled microcystin-LR terbium cryptate |
spellingShingle |
A fluorescent-labeled microcystin-LR terbium cryptate Oliveira,Eduardo J. A. terbium cryptate microcystin-LR cyanobacteria |
title_short |
A fluorescent-labeled microcystin-LR terbium cryptate |
title_full |
A fluorescent-labeled microcystin-LR terbium cryptate |
title_fullStr |
A fluorescent-labeled microcystin-LR terbium cryptate |
title_full_unstemmed |
A fluorescent-labeled microcystin-LR terbium cryptate |
title_sort |
A fluorescent-labeled microcystin-LR terbium cryptate |
author |
Oliveira,Eduardo J. A. |
author_facet |
Oliveira,Eduardo J. A. Vila Nova,Suzana P. Alves-Jr,Severino Santa-Cruz,Petrus Molica,Renato J. R. Teixeira,Antonio Malageño,Elizabete Lima Filho,José L. |
author_role |
author |
author2 |
Vila Nova,Suzana P. Alves-Jr,Severino Santa-Cruz,Petrus Molica,Renato J. R. Teixeira,Antonio Malageño,Elizabete Lima Filho,José L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Eduardo J. A. Vila Nova,Suzana P. Alves-Jr,Severino Santa-Cruz,Petrus Molica,Renato J. R. Teixeira,Antonio Malageño,Elizabete Lima Filho,José L. |
dc.subject.por.fl_str_mv |
terbium cryptate microcystin-LR cyanobacteria |
topic |
terbium cryptate microcystin-LR cyanobacteria |
description |
A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532006000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.17 n.2 2006 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318166965223424 |