Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000500015 |
Resumo: | In this paper, we present a simple and low-cost capillary electrophoresis method for ultra-fast determination of active ingredients present in three different pharmaceutical formulations: (i) scopolamine (SCO) and dipyrone (DIP); (ii) orphenadrine (ORP), caffeine (CAF) and DIP; (iii) mepyramine (MEP), CAF, DIP, and ascorbic acid (AA). The proposed method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The samples can be analyzed in a single run (< 1 minute) using 10 mmol L-1 3,4-dimethoxycinnamate (DMX) and 12 mmol L-1 triethanolamine (TEA) as a background electrolyte (pH = 8.5). A linear working range (r > 0.993) was obtained from 0.10 to 0.50 mmol L-1 for SCO, from 0.25 to 1.25 mmol L-1 for ORP and MEP, from 0.50 to 2.50 mmol L-1 for DIP and CAF, and from 1.00 to 3.00 mmol L-1 for AA. The limits of detection were 0.02, 0.05, 0.03, 0.04, 0.02, and 0.06 mmol L-1 for SCO, DIP, ORP, CAF, MEP, and AA, respectively. In addition, the proposed method also provided adequate intra (< 4%) and inter-day (< 14%) precision values, as well as satisfactory recovery values for spiked pharmaceutical samples (from 92% to 103%). |
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Journal of the Brazilian Chemical Society (Online) |
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Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detectioncapillary electrophoresisultra-fast analysismetamizoleC4Dmulticomponent analysisIn this paper, we present a simple and low-cost capillary electrophoresis method for ultra-fast determination of active ingredients present in three different pharmaceutical formulations: (i) scopolamine (SCO) and dipyrone (DIP); (ii) orphenadrine (ORP), caffeine (CAF) and DIP; (iii) mepyramine (MEP), CAF, DIP, and ascorbic acid (AA). The proposed method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The samples can be analyzed in a single run (< 1 minute) using 10 mmol L-1 3,4-dimethoxycinnamate (DMX) and 12 mmol L-1 triethanolamine (TEA) as a background electrolyte (pH = 8.5). A linear working range (r > 0.993) was obtained from 0.10 to 0.50 mmol L-1 for SCO, from 0.25 to 1.25 mmol L-1 for ORP and MEP, from 0.50 to 2.50 mmol L-1 for DIP and CAF, and from 1.00 to 3.00 mmol L-1 for AA. The limits of detection were 0.02, 0.05, 0.03, 0.04, 0.02, and 0.06 mmol L-1 for SCO, DIP, ORP, CAF, MEP, and AA, respectively. In addition, the proposed method also provided adequate intra (< 4%) and inter-day (< 14%) precision values, as well as satisfactory recovery values for spiked pharmaceutical samples (from 92% to 103%).Sociedade Brasileira de Química2014-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000500015Journal of the Brazilian Chemical Society v.25 n.5 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140061info:eu-repo/semantics/openAccessMarra,Mariana C.Silva,Polyana L.Muñoz,Rodrigo A. A.Richter,Eduardo M.eng2014-05-30T00:00:00Zoai:scielo:S0103-50532014000500015Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-05-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
title |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
spellingShingle |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection Marra,Mariana C. capillary electrophoresis ultra-fast analysis metamizole C4D multicomponent analysis |
title_short |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
title_full |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
title_fullStr |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
title_full_unstemmed |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
title_sort |
Ultra-fast determination of scopolamine, orphenadrine, mepyramine, caffeine, dipyrone, and ascorbic acid by capillary electrophoresis with capacitively coupled contactless conductivity detection |
author |
Marra,Mariana C. |
author_facet |
Marra,Mariana C. Silva,Polyana L. Muñoz,Rodrigo A. A. Richter,Eduardo M. |
author_role |
author |
author2 |
Silva,Polyana L. Muñoz,Rodrigo A. A. Richter,Eduardo M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Marra,Mariana C. Silva,Polyana L. Muñoz,Rodrigo A. A. Richter,Eduardo M. |
dc.subject.por.fl_str_mv |
capillary electrophoresis ultra-fast analysis metamizole C4D multicomponent analysis |
topic |
capillary electrophoresis ultra-fast analysis metamizole C4D multicomponent analysis |
description |
In this paper, we present a simple and low-cost capillary electrophoresis method for ultra-fast determination of active ingredients present in three different pharmaceutical formulations: (i) scopolamine (SCO) and dipyrone (DIP); (ii) orphenadrine (ORP), caffeine (CAF) and DIP; (iii) mepyramine (MEP), CAF, DIP, and ascorbic acid (AA). The proposed method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The samples can be analyzed in a single run (< 1 minute) using 10 mmol L-1 3,4-dimethoxycinnamate (DMX) and 12 mmol L-1 triethanolamine (TEA) as a background electrolyte (pH = 8.5). A linear working range (r > 0.993) was obtained from 0.10 to 0.50 mmol L-1 for SCO, from 0.25 to 1.25 mmol L-1 for ORP and MEP, from 0.50 to 2.50 mmol L-1 for DIP and CAF, and from 1.00 to 3.00 mmol L-1 for AA. The limits of detection were 0.02, 0.05, 0.03, 0.04, 0.02, and 0.06 mmol L-1 for SCO, DIP, ORP, CAF, MEP, and AA, respectively. In addition, the proposed method also provided adequate intra (< 4%) and inter-day (< 14%) precision values, as well as satisfactory recovery values for spiked pharmaceutical samples (from 92% to 103%). |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000500015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000500015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20140061 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.25 n.5 2014 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318176090980352 |