Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000300007 |
Resumo: | A method was developed and validated in-house for determination of ochratoxin A (OTA) in wine using charge coupled device (CCD) to acquire the fluorescence images of mycotoxin from high-performance thin-layer chromatography (HPTLC) under UV lamp. The method showed a mean recovery of 90.4%. The quantification and detection limist were 0.1 µg L-1 and 0.016 µg L-1 per spot, respectively. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify OTA in wine. The occurrence of OTA in Brazilian wines was evaluated. OTA was found in one sample at a level of 4.5 µg L-1, which is higher than the limit of 2 µg L-1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine. |
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Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled deviceochratoxin Awinecharge-coupled device imaginghigh-performance thin-layer chromatographyA method was developed and validated in-house for determination of ochratoxin A (OTA) in wine using charge coupled device (CCD) to acquire the fluorescence images of mycotoxin from high-performance thin-layer chromatography (HPTLC) under UV lamp. The method showed a mean recovery of 90.4%. The quantification and detection limist were 0.1 µg L-1 and 0.016 µg L-1 per spot, respectively. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify OTA in wine. The occurrence of OTA in Brazilian wines was evaluated. OTA was found in one sample at a level of 4.5 µg L-1, which is higher than the limit of 2 µg L-1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000300007Journal of the Brazilian Chemical Society v.21 n.3 2010reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532010000300007info:eu-repo/semantics/openAccessWelke,Juliane E.Hoeltz,MicheleDottori,Horacio A.Noll,Isa B.eng2010-04-30T00:00:00Zoai:scielo:S0103-50532010000300007Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2010-04-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
title |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
spellingShingle |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device Welke,Juliane E. ochratoxin A wine charge-coupled device imaging high-performance thin-layer chromatography |
title_short |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
title_full |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
title_fullStr |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
title_full_unstemmed |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
title_sort |
Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device |
author |
Welke,Juliane E. |
author_facet |
Welke,Juliane E. Hoeltz,Michele Dottori,Horacio A. Noll,Isa B. |
author_role |
author |
author2 |
Hoeltz,Michele Dottori,Horacio A. Noll,Isa B. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Welke,Juliane E. Hoeltz,Michele Dottori,Horacio A. Noll,Isa B. |
dc.subject.por.fl_str_mv |
ochratoxin A wine charge-coupled device imaging high-performance thin-layer chromatography |
topic |
ochratoxin A wine charge-coupled device imaging high-performance thin-layer chromatography |
description |
A method was developed and validated in-house for determination of ochratoxin A (OTA) in wine using charge coupled device (CCD) to acquire the fluorescence images of mycotoxin from high-performance thin-layer chromatography (HPTLC) under UV lamp. The method showed a mean recovery of 90.4%. The quantification and detection limist were 0.1 µg L-1 and 0.016 µg L-1 per spot, respectively. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify OTA in wine. The occurrence of OTA in Brazilian wines was evaluated. OTA was found in one sample at a level of 4.5 µg L-1, which is higher than the limit of 2 µg L-1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532010000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.21 n.3 2010 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318170657259520 |