Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids

Detalhes bibliográficos
Autor(a) principal: Guaratini,Thais
Data de Publicação: 2009
Outros Autores: Cardozo,Karina H. M., Pinto,Ernani, Colepicolo,Pio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000900007
Resumo: Qualitative and quantitative determination of carotenoids pigments can provide valuable information about the organisms in which this important class of compounds is found. In the HPLC analysis of pigments, diode array (DAD), electrochemical (ED) and other kinds of detector may be used. The aim of this work is to develop an HPLC method using a C30 column to identify and quantify sixteen different pigments from algae. A further aim is to compare precision and accuracy obtained by DAD and ED. ED is normally more sensible than DAD. On the other hand, the highest precision and accuracy was obtained with DAD. In conclusion, the method was efficient for quantitative and qualitative analyses of pigments from cyanobacteria and different microalgae classes. Their pigment patterns for several organisms are also discussed.
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spelling Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoidscarotenoidschlorophyllsHPLC-DADHPLC-EDalgaeQualitative and quantitative determination of carotenoids pigments can provide valuable information about the organisms in which this important class of compounds is found. In the HPLC analysis of pigments, diode array (DAD), electrochemical (ED) and other kinds of detector may be used. The aim of this work is to develop an HPLC method using a C30 column to identify and quantify sixteen different pigments from algae. A further aim is to compare precision and accuracy obtained by DAD and ED. ED is normally more sensible than DAD. On the other hand, the highest precision and accuracy was obtained with DAD. In conclusion, the method was efficient for quantitative and qualitative analyses of pigments from cyanobacteria and different microalgae classes. Their pigment patterns for several organisms are also discussed.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000900007Journal of the Brazilian Chemical Society v.20 n.9 2009reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532009000900007info:eu-repo/semantics/openAccessGuaratini,ThaisCardozo,Karina H. M.Pinto,ErnaniColepicolo,Pioeng2011-10-14T00:00:00Zoai:scielo:S0103-50532009000900007Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-10-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
title Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
spellingShingle Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
Guaratini,Thais
carotenoids
chlorophylls
HPLC-DAD
HPLC-ED
algae
title_short Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
title_full Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
title_fullStr Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
title_full_unstemmed Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
title_sort Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids
author Guaratini,Thais
author_facet Guaratini,Thais
Cardozo,Karina H. M.
Pinto,Ernani
Colepicolo,Pio
author_role author
author2 Cardozo,Karina H. M.
Pinto,Ernani
Colepicolo,Pio
author2_role author
author
author
dc.contributor.author.fl_str_mv Guaratini,Thais
Cardozo,Karina H. M.
Pinto,Ernani
Colepicolo,Pio
dc.subject.por.fl_str_mv carotenoids
chlorophylls
HPLC-DAD
HPLC-ED
algae
topic carotenoids
chlorophylls
HPLC-DAD
HPLC-ED
algae
description Qualitative and quantitative determination of carotenoids pigments can provide valuable information about the organisms in which this important class of compounds is found. In the HPLC analysis of pigments, diode array (DAD), electrochemical (ED) and other kinds of detector may be used. The aim of this work is to develop an HPLC method using a C30 column to identify and quantify sixteen different pigments from algae. A further aim is to compare precision and accuracy obtained by DAD and ED. ED is normally more sensible than DAD. On the other hand, the highest precision and accuracy was obtained with DAD. In conclusion, the method was efficient for quantitative and qualitative analyses of pigments from cyanobacteria and different microalgae classes. Their pigment patterns for several organisms are also discussed.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000900007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000900007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532009000900007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.20 n.9 2009
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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