Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000800015 |
Resumo: | This work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-picrylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies. |
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Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPOEPRTEMPOLDPPHDMPODFTThis work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-picrylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000800015Journal of the Brazilian Chemical Society v.20 n.8 2009reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532009000800015info:eu-repo/semantics/openAccessSantos,Adevailton Bernardo dosSilva,Dulce Helena SiqueiraBolzani,Vanderlan da SilvaSantos,Luciana ÁvilaSchmidt,Tomé MauroBaffa,Oswaldoeng2009-10-30T00:00:00Zoai:scielo:S0103-50532009000800015Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2009-10-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
title |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
spellingShingle |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO Santos,Adevailton Bernardo dos EPR TEMPOL DPPH DMPO DFT |
title_short |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
title_full |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
title_fullStr |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
title_full_unstemmed |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
title_sort |
Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO |
author |
Santos,Adevailton Bernardo dos |
author_facet |
Santos,Adevailton Bernardo dos Silva,Dulce Helena Siqueira Bolzani,Vanderlan da Silva Santos,Luciana Ávila Schmidt,Tomé Mauro Baffa,Oswaldo |
author_role |
author |
author2 |
Silva,Dulce Helena Siqueira Bolzani,Vanderlan da Silva Santos,Luciana Ávila Schmidt,Tomé Mauro Baffa,Oswaldo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Adevailton Bernardo dos Silva,Dulce Helena Siqueira Bolzani,Vanderlan da Silva Santos,Luciana Ávila Schmidt,Tomé Mauro Baffa,Oswaldo |
dc.subject.por.fl_str_mv |
EPR TEMPOL DPPH DMPO DFT |
topic |
EPR TEMPOL DPPH DMPO DFT |
description |
This work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-picrylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000800015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000800015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532009000800015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.20 n.8 2009 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318170233634816 |