Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO

Detalhes bibliográficos
Autor(a) principal: dos Santos, Adevailton Bernardo
Data de Publicação: 2009
Outros Autores: Siqueira Silva, Dulce Helena [UNESP], Bolzani, Vanderlan da Silva [UNESP], Santos, Luciana Avila [UNESP], Schmidt, Tome Mauro, Baffa, Oswaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0103-50532009000800015
http://hdl.handle.net/11449/26080
Resumo: This work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-pierylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH circle) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies.
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spelling Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPOEPRTEMPOLDPPHDMPODFTThis work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-pierylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH circle) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de Uberlândia (UFU), Fac Ciencias Integradas Pontol, BR-38302000 Ituiutaba, MG, BrazilUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilUniversidade Federal de Uberlândia (UFU), Inst Fis, BR-38400902 Uberlandia, MG, BrazilUniv São Paulo, Dept Fis & Matemat, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilSoc Brasileira QuimicaUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)dos Santos, Adevailton BernardoSiqueira Silva, Dulce Helena [UNESP]Bolzani, Vanderlan da Silva [UNESP]Santos, Luciana Avila [UNESP]Schmidt, Tome MauroBaffa, Oswaldo2014-05-20T14:20:14Z2014-05-20T14:20:14Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1483-1492http://dx.doi.org/10.1590/S0103-50532009000800015Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 20, n. 8, p. 1483-1492, 2009.0103-5053http://hdl.handle.net/11449/2608010.1590/S0103-50532009000800015S0103-50532009000800015WOS:000271226900015S0103-50532009000800015.pdf47020049042312484484083685251673Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Brazilian Chemical Society1.4440,357info:eu-repo/semantics/openAccess2021-10-23T17:09:48Zoai:repositorio.unesp.br:11449/26080Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:21:02.054365Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
title Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
spellingShingle Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
dos Santos, Adevailton Bernardo
EPR
TEMPOL
DPPH
DMPO
DFT
title_short Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
title_full Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
title_fullStr Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
title_full_unstemmed Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
title_sort Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
author dos Santos, Adevailton Bernardo
author_facet dos Santos, Adevailton Bernardo
Siqueira Silva, Dulce Helena [UNESP]
Bolzani, Vanderlan da Silva [UNESP]
Santos, Luciana Avila [UNESP]
Schmidt, Tome Mauro
Baffa, Oswaldo
author_role author
author2 Siqueira Silva, Dulce Helena [UNESP]
Bolzani, Vanderlan da Silva [UNESP]
Santos, Luciana Avila [UNESP]
Schmidt, Tome Mauro
Baffa, Oswaldo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv dos Santos, Adevailton Bernardo
Siqueira Silva, Dulce Helena [UNESP]
Bolzani, Vanderlan da Silva [UNESP]
Santos, Luciana Avila [UNESP]
Schmidt, Tome Mauro
Baffa, Oswaldo
dc.subject.por.fl_str_mv EPR
TEMPOL
DPPH
DMPO
DFT
topic EPR
TEMPOL
DPPH
DMPO
DFT
description This work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-pierylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH circle) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2014-05-20T14:20:14Z
2014-05-20T14:20:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0103-50532009000800015
Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 20, n. 8, p. 1483-1492, 2009.
0103-5053
http://hdl.handle.net/11449/26080
10.1590/S0103-50532009000800015
S0103-50532009000800015
WOS:000271226900015
S0103-50532009000800015.pdf
4702004904231248
4484083685251673
url http://dx.doi.org/10.1590/S0103-50532009000800015
http://hdl.handle.net/11449/26080
identifier_str_mv Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 20, n. 8, p. 1483-1492, 2009.
0103-5053
10.1590/S0103-50532009000800015
S0103-50532009000800015
WOS:000271226900015
S0103-50532009000800015.pdf
4702004904231248
4484083685251673
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Brazilian Chemical Society
1.444
0,357
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1483-1492
dc.publisher.none.fl_str_mv Soc Brasileira Quimica
publisher.none.fl_str_mv Soc Brasileira Quimica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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