Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0103-50532009000800015 http://hdl.handle.net/11449/26080 |
Resumo: | This work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-pierylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH circle) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies. |
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Repositório Institucional da UNESP |
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Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPOEPRTEMPOLDPPHDMPODFTThis work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-pierylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH circle) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de Uberlândia (UFU), Fac Ciencias Integradas Pontol, BR-38302000 Ituiutaba, MG, BrazilUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilUniversidade Federal de Uberlândia (UFU), Inst Fis, BR-38400902 Uberlandia, MG, BrazilUniv São Paulo, Dept Fis & Matemat, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilSoc Brasileira QuimicaUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)dos Santos, Adevailton BernardoSiqueira Silva, Dulce Helena [UNESP]Bolzani, Vanderlan da Silva [UNESP]Santos, Luciana Avila [UNESP]Schmidt, Tome MauroBaffa, Oswaldo2014-05-20T14:20:14Z2014-05-20T14:20:14Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1483-1492http://dx.doi.org/10.1590/S0103-50532009000800015Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 20, n. 8, p. 1483-1492, 2009.0103-5053http://hdl.handle.net/11449/2608010.1590/S0103-50532009000800015S0103-50532009000800015WOS:000271226900015S0103-50532009000800015.pdf47020049042312484484083685251673Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Brazilian Chemical Society1.4440,357info:eu-repo/semantics/openAccess2021-10-23T17:09:48Zoai:repositorio.unesp.br:11449/26080Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:21:02.054365Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
title |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
spellingShingle |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO dos Santos, Adevailton Bernardo EPR TEMPOL DPPH DMPO DFT |
title_short |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
title_full |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
title_fullStr |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
title_full_unstemmed |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
title_sort |
Antioxidant Properties of Plant Extracts: an EPR and DFT Comparative Study of the Reaction with DPPH, TEMPOL and Spin Trap DMPO |
author |
dos Santos, Adevailton Bernardo |
author_facet |
dos Santos, Adevailton Bernardo Siqueira Silva, Dulce Helena [UNESP] Bolzani, Vanderlan da Silva [UNESP] Santos, Luciana Avila [UNESP] Schmidt, Tome Mauro Baffa, Oswaldo |
author_role |
author |
author2 |
Siqueira Silva, Dulce Helena [UNESP] Bolzani, Vanderlan da Silva [UNESP] Santos, Luciana Avila [UNESP] Schmidt, Tome Mauro Baffa, Oswaldo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
dos Santos, Adevailton Bernardo Siqueira Silva, Dulce Helena [UNESP] Bolzani, Vanderlan da Silva [UNESP] Santos, Luciana Avila [UNESP] Schmidt, Tome Mauro Baffa, Oswaldo |
dc.subject.por.fl_str_mv |
EPR TEMPOL DPPH DMPO DFT |
topic |
EPR TEMPOL DPPH DMPO DFT |
description |
This work presents a comparative study of the antioxidant activity of extracts from nine plant species belonging to the Brazilian flora - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - acting on the free-radicals DPPH (2,2-diphenyl-1-pierylhydrazyl) and TEMPOL (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy-1-oxyl) by electron paramagnetic resonance (EPR), and acting on the hydroxyl radical (OH circle) by the spin-trapping technique generated by a Fenton reaction. Results showed that the extracts of Iryanthera juruensis and Chrysophyllum inornatum display the strongest antioxidant activities. The results of scavenging tests were clarified by computational calculations - density functional theory (DFT), local density approximation (LDA) with SIESTA code - indicating that the energy released in the reduction reaction of TEMPOL is less than DPPH. Due to its availability and cost DPPH is more often used in these tests than TEMPOL, however TEMPOL should be considered when studying processes dealing with smaller energies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2014-05-20T14:20:14Z 2014-05-20T14:20:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0103-50532009000800015 Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 20, n. 8, p. 1483-1492, 2009. 0103-5053 http://hdl.handle.net/11449/26080 10.1590/S0103-50532009000800015 S0103-50532009000800015 WOS:000271226900015 S0103-50532009000800015.pdf 4702004904231248 4484083685251673 |
url |
http://dx.doi.org/10.1590/S0103-50532009000800015 http://hdl.handle.net/11449/26080 |
identifier_str_mv |
Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 20, n. 8, p. 1483-1492, 2009. 0103-5053 10.1590/S0103-50532009000800015 S0103-50532009000800015 WOS:000271226900015 S0103-50532009000800015.pdf 4702004904231248 4484083685251673 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the Brazilian Chemical Society 1.444 0,357 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1483-1492 |
dc.publisher.none.fl_str_mv |
Soc Brasileira Quimica |
publisher.none.fl_str_mv |
Soc Brasileira Quimica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129191354302464 |