A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000601278 |
Resumo: | We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). |
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A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading MicroorganismsbiocatalysisFenton reactionfluorescencemicroorganism screeningPET degradationWe propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).Sociedade Brasileira de Química2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000601278Journal of the Brazilian Chemical Society v.29 n.6 2018reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20170224info:eu-repo/semantics/openAccessChaves,Michel R. B.Lima,Maria L. S. O.Malafatti-Picca,Lusianede Angelis,Derlene A.Castro,Aline M. deValoni,ÉrikaMarsaioli,Anita J.eng2018-05-18T00:00:00Zoai:scielo:S0103-50532018000601278Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2018-05-18T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
title |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
spellingShingle |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms Chaves,Michel R. B. biocatalysis Fenton reaction fluorescence microorganism screening PET degradation |
title_short |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
title_full |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
title_fullStr |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
title_full_unstemmed |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
title_sort |
A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms |
author |
Chaves,Michel R. B. |
author_facet |
Chaves,Michel R. B. Lima,Maria L. S. O. Malafatti-Picca,Lusiane de Angelis,Derlene A. Castro,Aline M. de Valoni,Érika Marsaioli,Anita J. |
author_role |
author |
author2 |
Lima,Maria L. S. O. Malafatti-Picca,Lusiane de Angelis,Derlene A. Castro,Aline M. de Valoni,Érika Marsaioli,Anita J. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Chaves,Michel R. B. Lima,Maria L. S. O. Malafatti-Picca,Lusiane de Angelis,Derlene A. Castro,Aline M. de Valoni,Érika Marsaioli,Anita J. |
dc.subject.por.fl_str_mv |
biocatalysis Fenton reaction fluorescence microorganism screening PET degradation |
topic |
biocatalysis Fenton reaction fluorescence microorganism screening PET degradation |
description |
We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000601278 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000601278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20170224 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.29 n.6 2018 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318180814815232 |