A novel approach to cold vapor generation for the determination of mercury in biological samples
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000500018 |
Resumo: | A novel approach to the generation of mercury vapor at high pH by the simple addition of NaOH or other base to a solution containing mercury ions is described. Subsequent addition of another reducing agent, such as NaBH4 or SnCl2, is unnecessary. Following total dissolution of sample using HNO3 and H2O2 in a closed microwave vessel, the pH of the sample solution is increased to 13 by the addition of NaOH. After standing for 90 min in a closed vessel, the Hg0 is directed to the plasma by a flow of argon. Emission from mercury is measured at 253.652 nm by an axial view inductively coupled plasma optical emission spectrometer (ICP OES). The procedure was applied to five certified biological samples, yielding a detection limit (3s, n = 10) of 0.04 µg g-1 based on a nominal sample mass of 0.5 g in a final volume of 50 mL. Calibration was achieved using simple aqueous standard solutions containing 0.1 mol L-1 NaOH. The procedure was efficient, with determined values lying in the range of 85-113 % of the certified values, showing good agreement at the 95% confidence level (t-test). The precision was fit for purpose, with relative standard deviations ranging from 7 to 9%. Organomercury species in solution were not detected; only Hg2+ in solution produces a signal. This new procedure provides for a simple approach to quantitation (and potentially speciation). |
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A novel approach to cold vapor generation for the determination of mercury in biological samplesmercuryalkaline pHinductively coupled plasma optical emission spectrometrycold vapor generationbiological samplesA novel approach to the generation of mercury vapor at high pH by the simple addition of NaOH or other base to a solution containing mercury ions is described. Subsequent addition of another reducing agent, such as NaBH4 or SnCl2, is unnecessary. Following total dissolution of sample using HNO3 and H2O2 in a closed microwave vessel, the pH of the sample solution is increased to 13 by the addition of NaOH. After standing for 90 min in a closed vessel, the Hg0 is directed to the plasma by a flow of argon. Emission from mercury is measured at 253.652 nm by an axial view inductively coupled plasma optical emission spectrometer (ICP OES). The procedure was applied to five certified biological samples, yielding a detection limit (3s, n = 10) of 0.04 µg g-1 based on a nominal sample mass of 0.5 g in a final volume of 50 mL. Calibration was achieved using simple aqueous standard solutions containing 0.1 mol L-1 NaOH. The procedure was efficient, with determined values lying in the range of 85-113 % of the certified values, showing good agreement at the 95% confidence level (t-test). The precision was fit for purpose, with relative standard deviations ranging from 7 to 9%. Organomercury species in solution were not detected; only Hg2+ in solution produces a signal. This new procedure provides for a simple approach to quantitation (and potentially speciation).Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000500018Journal of the Brazilian Chemical Society v.19 n.5 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000500018info:eu-repo/semantics/openAccessSantos,Eder José dosHerrmann,Amanda BeatrizFrescura,Vera Lúcia AzzolinSturgeon,Ralph EdwardCurtius,Adilson Joséeng2008-08-05T00:00:00Zoai:scielo:S0103-50532008000500018Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2008-08-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
title |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
spellingShingle |
A novel approach to cold vapor generation for the determination of mercury in biological samples Santos,Eder José dos mercury alkaline pH inductively coupled plasma optical emission spectrometry cold vapor generation biological samples |
title_short |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
title_full |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
title_fullStr |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
title_full_unstemmed |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
title_sort |
A novel approach to cold vapor generation for the determination of mercury in biological samples |
author |
Santos,Eder José dos |
author_facet |
Santos,Eder José dos Herrmann,Amanda Beatriz Frescura,Vera Lúcia Azzolin Sturgeon,Ralph Edward Curtius,Adilson José |
author_role |
author |
author2 |
Herrmann,Amanda Beatriz Frescura,Vera Lúcia Azzolin Sturgeon,Ralph Edward Curtius,Adilson José |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Santos,Eder José dos Herrmann,Amanda Beatriz Frescura,Vera Lúcia Azzolin Sturgeon,Ralph Edward Curtius,Adilson José |
dc.subject.por.fl_str_mv |
mercury alkaline pH inductively coupled plasma optical emission spectrometry cold vapor generation biological samples |
topic |
mercury alkaline pH inductively coupled plasma optical emission spectrometry cold vapor generation biological samples |
description |
A novel approach to the generation of mercury vapor at high pH by the simple addition of NaOH or other base to a solution containing mercury ions is described. Subsequent addition of another reducing agent, such as NaBH4 or SnCl2, is unnecessary. Following total dissolution of sample using HNO3 and H2O2 in a closed microwave vessel, the pH of the sample solution is increased to 13 by the addition of NaOH. After standing for 90 min in a closed vessel, the Hg0 is directed to the plasma by a flow of argon. Emission from mercury is measured at 253.652 nm by an axial view inductively coupled plasma optical emission spectrometer (ICP OES). The procedure was applied to five certified biological samples, yielding a detection limit (3s, n = 10) of 0.04 µg g-1 based on a nominal sample mass of 0.5 g in a final volume of 50 mL. Calibration was achieved using simple aqueous standard solutions containing 0.1 mol L-1 NaOH. The procedure was efficient, with determined values lying in the range of 85-113 % of the certified values, showing good agreement at the 95% confidence level (t-test). The precision was fit for purpose, with relative standard deviations ranging from 7 to 9%. Organomercury species in solution were not detected; only Hg2+ in solution produces a signal. This new procedure provides for a simple approach to quantitation (and potentially speciation). |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000500018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000500018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532008000500018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.19 n.5 2008 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318169009946624 |