Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases

Detalhes bibliográficos
Autor(a) principal: Bicalho,Beatriz
Data de Publicação: 2004
Outros Autores: Chen,Lu S., Grognux,Johann, Reymond,Jean-Louis, Marsaioli,Anita J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000600019
Resumo: Biocatalysis reactions were performed on microtiter plates (200 µL) aiming at the utilization of fluorogenic substrates (100 µmol L-1) for rapid whole cell screening for epoxide hydrolases (EHs) and Baeyer-Villiger monoxygenases (BVMOs). A final protocol was achieved for EHs, with 3 new enzymatic sources being detected (Agrobacterium tumefaciens, Pichia stipitis, Trichosporom cutaneum). The fluorogenic assay for BVMO did not work as expected. However, an approach to possible variables involved (aeration; pH) provided the first detection of a BVMO activity in T. cutaneum.
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spelling Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenasesmicroorganismsBaeyer-Villiger monoxygenaseepoxide hydrolaseBiocatalysis reactions were performed on microtiter plates (200 µL) aiming at the utilization of fluorogenic substrates (100 µmol L-1) for rapid whole cell screening for epoxide hydrolases (EHs) and Baeyer-Villiger monoxygenases (BVMOs). A final protocol was achieved for EHs, with 3 new enzymatic sources being detected (Agrobacterium tumefaciens, Pichia stipitis, Trichosporom cutaneum). The fluorogenic assay for BVMO did not work as expected. However, an approach to possible variables involved (aeration; pH) provided the first detection of a BVMO activity in T. cutaneum.Sociedade Brasileira de Química2004-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000600019Journal of the Brazilian Chemical Society v.15 n.6 2004reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532004000600019info:eu-repo/semantics/openAccessBicalho,BeatrizChen,Lu S.Grognux,JohannReymond,Jean-LouisMarsaioli,Anita J.eng2005-01-24T00:00:00Zoai:scielo:S0103-50532004000600019Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2005-01-24T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
title Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
spellingShingle Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
Bicalho,Beatriz
microorganisms
Baeyer-Villiger monoxygenase
epoxide hydrolase
title_short Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
title_full Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
title_fullStr Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
title_full_unstemmed Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
title_sort Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
author Bicalho,Beatriz
author_facet Bicalho,Beatriz
Chen,Lu S.
Grognux,Johann
Reymond,Jean-Louis
Marsaioli,Anita J.
author_role author
author2 Chen,Lu S.
Grognux,Johann
Reymond,Jean-Louis
Marsaioli,Anita J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bicalho,Beatriz
Chen,Lu S.
Grognux,Johann
Reymond,Jean-Louis
Marsaioli,Anita J.
dc.subject.por.fl_str_mv microorganisms
Baeyer-Villiger monoxygenase
epoxide hydrolase
topic microorganisms
Baeyer-Villiger monoxygenase
epoxide hydrolase
description Biocatalysis reactions were performed on microtiter plates (200 µL) aiming at the utilization of fluorogenic substrates (100 µmol L-1) for rapid whole cell screening for epoxide hydrolases (EHs) and Baeyer-Villiger monoxygenases (BVMOs). A final protocol was achieved for EHs, with 3 new enzymatic sources being detected (Agrobacterium tumefaciens, Pichia stipitis, Trichosporom cutaneum). The fluorogenic assay for BVMO did not work as expected. However, an approach to possible variables involved (aeration; pH) provided the first detection of a BVMO activity in T. cutaneum.
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000600019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000600019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532004000600019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.15 n.6 2004
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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