Rapid Determination of Vitamins D2 and D3 in Dairy Products by High-Performance Liquid Chromatography after Solid Phase Extraction Based on Polystyrene/Polypyrrole Composite Nanofibers

• Autor(a) principal: Han,Qing
• Data de Publicação: 2020
• Outros Autores: Wei,Lanlan, Deng,Guozhe, Sun,Ying, Deng,Jianjun, Tang,Zigang, Fan,Jinhui, Wang,Xia, Kang,Xuejun
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000601216
• topic: nanofibers; solid-phase extraction; vitamin D2; vitamin D3; dairy products

The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D2 (D2) and vitamin D3 (D3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL−1 in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D2 and D3 in dairy products.