Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study

Detalhes bibliográficos
Autor(a) principal: Silva,Vania C. F. da
Data de Publicação: 2008
Outros Autores: Contesini,Fabiano J., Carvalho,Patrícia de O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000800005
Resumo: Aspergillus niger lipase was immobilized by deposition on Celite and the hydrolytic and esterification activities, stability and enantioselectivity of both free and immobilized enzyme were compared. Both the free and immobilized preparations showed similar biochemical properties, with maximum activity at pH 6.0 and a temperature of 30-40 ºC. The most important effects observed when the lipase was immobilized were thermal stability and an improved esterification activity during the reaction of (R,S)-ibuprofen with 1-propanol in isooctane. Moreover, immobilized Aspergillus niger lipase maintained an esterification activity of at least 73% after 5 days of storage at 40 ºC and can be recycled and reused at least 6 times.
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spelling Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative studyimmobilizationlipaseibuprofenAspergillus niger lipase was immobilized by deposition on Celite and the hydrolytic and esterification activities, stability and enantioselectivity of both free and immobilized enzyme were compared. Both the free and immobilized preparations showed similar biochemical properties, with maximum activity at pH 6.0 and a temperature of 30-40 ºC. The most important effects observed when the lipase was immobilized were thermal stability and an improved esterification activity during the reaction of (R,S)-ibuprofen with 1-propanol in isooctane. Moreover, immobilized Aspergillus niger lipase maintained an esterification activity of at least 73% after 5 days of storage at 40 ºC and can be recycled and reused at least 6 times.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000800005Journal of the Brazilian Chemical Society v.19 n.8 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000800005info:eu-repo/semantics/openAccessSilva,Vania C. F. daContesini,Fabiano J.Carvalho,Patrícia de O.eng2008-12-09T00:00:00Zoai:scielo:S0103-50532008000800005Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2008-12-09T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
title Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
spellingShingle Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
Silva,Vania C. F. da
immobilization
lipase
ibuprofen
title_short Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
title_full Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
title_fullStr Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
title_full_unstemmed Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
title_sort Characterization and catalytic activity of free and immobilized lipase from Aspergillus niger: a comparative study
author Silva,Vania C. F. da
author_facet Silva,Vania C. F. da
Contesini,Fabiano J.
Carvalho,Patrícia de O.
author_role author
author2 Contesini,Fabiano J.
Carvalho,Patrícia de O.
author2_role author
author
dc.contributor.author.fl_str_mv Silva,Vania C. F. da
Contesini,Fabiano J.
Carvalho,Patrícia de O.
dc.subject.por.fl_str_mv immobilization
lipase
ibuprofen
topic immobilization
lipase
ibuprofen
description Aspergillus niger lipase was immobilized by deposition on Celite and the hydrolytic and esterification activities, stability and enantioselectivity of both free and immobilized enzyme were compared. Both the free and immobilized preparations showed similar biochemical properties, with maximum activity at pH 6.0 and a temperature of 30-40 ºC. The most important effects observed when the lipase was immobilized were thermal stability and an improved esterification activity during the reaction of (R,S)-ibuprofen with 1-propanol in isooctane. Moreover, immobilized Aspergillus niger lipase maintained an esterification activity of at least 73% after 5 days of storage at 40 ºC and can be recycled and reused at least 6 times.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000800005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000800005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532008000800005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.19 n.8 2008
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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