Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles

Detalhes bibliográficos
Autor(a) principal: Bruno,Laura Maria
Data de Publicação: 2008
Outros Autores: Lima Filho,José Luiz de, Castro,Heizir Ferreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003
Resumo: Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also obtained through chemical composition (FTIR).
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spelling Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particlesCandida lipaseMucor lipasehybrid matriximmobilizationMicrobial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also obtained through chemical composition (FTIR).Instituto de Tecnologia do Paraná - Tecpar2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003Brazilian Archives of Biology and Technology v.51 n.5 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000500003info:eu-repo/semantics/openAccessBruno,Laura MariaLima Filho,José Luiz deCastro,Heizir Ferreira deeng2008-10-29T00:00:00Zoai:scielo:S1516-89132008000500003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-10-29T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
title Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
spellingShingle Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
Bruno,Laura Maria
Candida lipase
Mucor lipase
hybrid matrix
immobilization
title_short Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
title_full Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
title_fullStr Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
title_full_unstemmed Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
title_sort Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
author Bruno,Laura Maria
author_facet Bruno,Laura Maria
Lima Filho,José Luiz de
Castro,Heizir Ferreira de
author_role author
author2 Lima Filho,José Luiz de
Castro,Heizir Ferreira de
author2_role author
author
dc.contributor.author.fl_str_mv Bruno,Laura Maria
Lima Filho,José Luiz de
Castro,Heizir Ferreira de
dc.subject.por.fl_str_mv Candida lipase
Mucor lipase
hybrid matrix
immobilization
topic Candida lipase
Mucor lipase
hybrid matrix
immobilization
description Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also obtained through chemical composition (FTIR).
publishDate 2008
dc.date.none.fl_str_mv 2008-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000500003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.5 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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