Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

• Autor(a) principal: Camargo,Camila L. de
• Data de Publicação: 2017
• Outros Autores: Vicentini,Marcia B. R., Gobbi,Angelo L., Martinez,Diego S. T., Lima,Renato S.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000400689
• topic: rapid test; albumin; human plasma; colorimetry; negatoscope

We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone's users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%.