Chemical composition and evaluation of antibacterial and antioxidant activities of the essential oil of Croton urucurana Baillon (Euphorbiaceae) stem bark

• Autor(a) principal: Simionatto,Euclésio
• Data de Publicação: 2007
• Outros Autores: Bonani,Vanderléa F. L., Morel,Ademir Farias, Poppi,Nilva Ré, Raposo Júnior,Jorge Luiz, Stuker,Caroline Z., Peruzzo,Gisele M., Peres,Marize T. L. P., Hess,Sônia C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000500002
• topic: Croton urucurana; essential oil; antioxidant; DPPH assay; antimicrobial acticity

The essential oil obtained from the stem bark of Croton urucurana Baillon (Euphorbiaceae) was analysed by GC and GC-MS. 83 compounds were identified and borneol (14.7%), bornyl acetate (5.2%), 1-isopropyl-7-methyl-4-methylene-1,3,4,5,6,8-hexahydro-2H-naphthalen-4a-ol (14.7%), sesquicineole (10.5%) and gamma-gurjunene epoxide (5.4%) were the main components. The EC50 value of the crude essential oil in the DPPH free radical scavenging assay was 3.21 mg mL-1. The fraction of the crude essential oil that presented antioxidant activity was purified by prep-TLC on silica gel. GC and GC-MS analysis revealed that alpha-bisabolol (38.3%), alpha-eudesmol (9.3%) and guaiol (8.2%) were the main components of the antioxidant fraction. The EC50 value measured for the bioactive oil fraction in the DPPH assay was 1.05 mg mL-1. The antimicrobial activity of the crude essential oil was assayed against seven Gram-positive and Gram-negative bacteria and three yeasts. Measured MIC values ranged from 1.25 to 10.00 mg mL-1.