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A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy

Bibliographic Details
Main Author: Mor,Natalia Cristina
Publication Date: 2020
Other Authors: Correia,Banny S. B., Val,Adalberto Luis, Tasic,Ljubica
Format: Article
Language: eng
Source: Journal of the Brazilian Chemical Society (Online)
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000400662
Summary: This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods.
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spelling A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopylipid analysisAmazonian fishnuclear magnetic resonance spectroscopyThis article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods.Sociedade Brasileira de Química2020-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000400662Journal of the Brazilian Chemical Society v.31 n.4 2020reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20190230info:eu-repo/semantics/openAccessMor,Natalia CristinaCorreia,Banny S. B.Val,Adalberto LuisTasic,Ljubicaeng2020-03-19T00:00:00Zoai:scielo:S0103-50532020000400662Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2020-03-19T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
title A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
spellingShingle A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
Mor,Natalia Cristina
lipid analysis
Amazonian fish
nuclear magnetic resonance spectroscopy
title_short A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
title_full A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
title_fullStr A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
title_full_unstemmed A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
title_sort A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
author Mor,Natalia Cristina
author_facet Mor,Natalia Cristina
Correia,Banny S. B.
Val,Adalberto Luis
Tasic,Ljubica
author_role author
author2 Correia,Banny S. B.
Val,Adalberto Luis
Tasic,Ljubica
author2_role author
author
author
dc.contributor.author.fl_str_mv Mor,Natalia Cristina
Correia,Banny S. B.
Val,Adalberto Luis
Tasic,Ljubica
dc.subject.por.fl_str_mv lipid analysis
Amazonian fish
nuclear magnetic resonance spectroscopy
topic lipid analysis
Amazonian fish
nuclear magnetic resonance spectroscopy
description This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000400662
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000400662
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0103-5053.20190230
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.31 n.4 2020
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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