Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design

Detalhes bibliográficos
Autor(a) principal: Cano,Cristiane B.
Data de Publicação: 2006
Outros Autores: Felsner,Maria L., Bruns,Roy E., Matos,Jivaldo R., Almeida-Muradian,Lígia B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000300024
Resumo: A mixture design was used to optimize the mobile phase composition for separation of carbohydrates in honey by High Performance Liquid Chromatography. Nine ternary acetronitrile, water and ethyl acetate mobile phases corresponding to a simplex centroid design with axial points were test to separate those carbohydrates most frequently encountered in honey samples. The results suggest that a special cubic model accurately describes changes in the proportions of these solvents in the mobile phase close to the region of optimal peak separation. The addition of a third solvent, ethyl acetate, to binary mobile phase of water and acetonitrile permitted a significant improvement in carbohydrate separation that can be applied to the quantitative analysis of these compounds in honey.
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spelling Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture designmobile phase optimizationHPLCcarbohydratesmixture designhoneyA mixture design was used to optimize the mobile phase composition for separation of carbohydrates in honey by High Performance Liquid Chromatography. Nine ternary acetronitrile, water and ethyl acetate mobile phases corresponding to a simplex centroid design with axial points were test to separate those carbohydrates most frequently encountered in honey samples. The results suggest that a special cubic model accurately describes changes in the proportions of these solvents in the mobile phase close to the region of optimal peak separation. The addition of a third solvent, ethyl acetate, to binary mobile phase of water and acetonitrile permitted a significant improvement in carbohydrate separation that can be applied to the quantitative analysis of these compounds in honey.Sociedade Brasileira de Química2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000300024Journal of the Brazilian Chemical Society v.17 n.3 2006reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532006000300024info:eu-repo/semantics/openAccessCano,Cristiane B.Felsner,Maria L.Bruns,Roy E.Matos,Jivaldo R.Almeida-Muradian,Lígia B.eng2006-05-25T00:00:00Zoai:scielo:S0103-50532006000300024Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2006-05-25T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
title Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
spellingShingle Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
Cano,Cristiane B.
mobile phase optimization
HPLC
carbohydrates
mixture design
honey
title_short Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
title_full Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
title_fullStr Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
title_full_unstemmed Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
title_sort Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design
author Cano,Cristiane B.
author_facet Cano,Cristiane B.
Felsner,Maria L.
Bruns,Roy E.
Matos,Jivaldo R.
Almeida-Muradian,Lígia B.
author_role author
author2 Felsner,Maria L.
Bruns,Roy E.
Matos,Jivaldo R.
Almeida-Muradian,Lígia B.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cano,Cristiane B.
Felsner,Maria L.
Bruns,Roy E.
Matos,Jivaldo R.
Almeida-Muradian,Lígia B.
dc.subject.por.fl_str_mv mobile phase optimization
HPLC
carbohydrates
mixture design
honey
topic mobile phase optimization
HPLC
carbohydrates
mixture design
honey
description A mixture design was used to optimize the mobile phase composition for separation of carbohydrates in honey by High Performance Liquid Chromatography. Nine ternary acetronitrile, water and ethyl acetate mobile phases corresponding to a simplex centroid design with axial points were test to separate those carbohydrates most frequently encountered in honey samples. The results suggest that a special cubic model accurately describes changes in the proportions of these solvents in the mobile phase close to the region of optimal peak separation. The addition of a third solvent, ethyl acetate, to binary mobile phase of water and acetonitrile permitted a significant improvement in carbohydrate separation that can be applied to the quantitative analysis of these compounds in honey.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000300024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000300024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532006000300024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.17 n.3 2006
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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