Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600006 |
Resumo: | The purpose of this work was to evaluate the applicability of two enzymatic methods to quantify glucose and sucrose in aqueous solutions with chitosan. The analytical methods were validated and the main parameters, as limit of detection, linearity range, precision and accuracy were determined. The global uncertainty for glucose enzymatic method was less than 10% for concentration levels between 700 and 2000 mg L-1, and for the sucrose enzymatic method, the global uncertainty showed values less than 3% for all concentration levels analyzed (100-1000 mg L-1). Despite the similarities of performances with respect to other analytical methods, the enzymatic method proved to be better than others, in particular high performance liquid-chromatography (HPLC), mainly if a great number of samples needs to be analyzed, allowing a quick result, and because the mucoadhesive properties of chitosan make difficult the HPLC analytical methodology, creating stability problems in the chromatographic column. |
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Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methodsuncertaintyenzymatic methodsglucosesucrosechitosanThe purpose of this work was to evaluate the applicability of two enzymatic methods to quantify glucose and sucrose in aqueous solutions with chitosan. The analytical methods were validated and the main parameters, as limit of detection, linearity range, precision and accuracy were determined. The global uncertainty for glucose enzymatic method was less than 10% for concentration levels between 700 and 2000 mg L-1, and for the sucrose enzymatic method, the global uncertainty showed values less than 3% for all concentration levels analyzed (100-1000 mg L-1). Despite the similarities of performances with respect to other analytical methods, the enzymatic method proved to be better than others, in particular high performance liquid-chromatography (HPLC), mainly if a great number of samples needs to be analyzed, allowing a quick result, and because the mucoadhesive properties of chitosan make difficult the HPLC analytical methodology, creating stability problems in the chromatographic column.Sociedade Brasileira de Química2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600006Journal of the Brazilian Chemical Society v.24 n.6 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130119info:eu-repo/semantics/openAccessEstevinho,Berta N.Ferraz,AméliaSantos,LúciaRocha,FernandoAlves,Armindaeng2013-06-27T00:00:00Zoai:scielo:S0103-50532013000600006Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-06-27T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
title |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
spellingShingle |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods Estevinho,Berta N. uncertainty enzymatic methods glucose sucrose chitosan |
title_short |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
title_full |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
title_fullStr |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
title_full_unstemmed |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
title_sort |
Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods |
author |
Estevinho,Berta N. |
author_facet |
Estevinho,Berta N. Ferraz,Amélia Santos,Lúcia Rocha,Fernando Alves,Arminda |
author_role |
author |
author2 |
Ferraz,Amélia Santos,Lúcia Rocha,Fernando Alves,Arminda |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Estevinho,Berta N. Ferraz,Amélia Santos,Lúcia Rocha,Fernando Alves,Arminda |
dc.subject.por.fl_str_mv |
uncertainty enzymatic methods glucose sucrose chitosan |
topic |
uncertainty enzymatic methods glucose sucrose chitosan |
description |
The purpose of this work was to evaluate the applicability of two enzymatic methods to quantify glucose and sucrose in aqueous solutions with chitosan. The analytical methods were validated and the main parameters, as limit of detection, linearity range, precision and accuracy were determined. The global uncertainty for glucose enzymatic method was less than 10% for concentration levels between 700 and 2000 mg L-1, and for the sucrose enzymatic method, the global uncertainty showed values less than 3% for all concentration levels analyzed (100-1000 mg L-1). Despite the similarities of performances with respect to other analytical methods, the enzymatic method proved to be better than others, in particular high performance liquid-chromatography (HPLC), mainly if a great number of samples needs to be analyzed, allowing a quick result, and because the mucoadhesive properties of chitosan make difficult the HPLC analytical methodology, creating stability problems in the chromatographic column. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20130119 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.24 n.6 2013 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318174881972224 |